LOCUS       sequence-267837-        8328 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of rat Myo 1c (Myr 2) in mammalian cells. N-terminal 
            GFP-tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8328)
  AUTHORS   Ruppert C, Godel J, Muller RT, Kroschewski R, Reinhard J, Bahler M
  TITLE     Localization of the rat myosin I molecules myr 1 and myr 2 and in 
            vivo targeting of their tail domains.
  JOURNAL   J Cell Sci. 1995 Dec;108 ( Pt 12):3775-86.
  PUBMED    8719884
REFERENCE   2  (bases 1 to 8328)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "1995-12"; volume: "108 ( Pt 12)"; pages: "3775-86"
COMMENT     Sequence Label: GFP-Myo 1c
FEATURES             Location/Qualifiers
     source          1..8328
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(658..679)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(919..938)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1266..1287
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4988..5004)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4988..5004)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    5116..5237
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5153..5172)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5207..5226
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(5244..5699)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5381..5402)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5593..5612
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5726..5830
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5832..6189
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5832..6023
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5855..5875)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6040..6175
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6224..7018
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             6224..7018
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6278..6297)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6888..6907
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(7206..7225)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7250..7297
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7626..8214
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8115..8134
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc
     1381 atggagagcc ccttgactgc ccgagaccgg gtgggggtgc aagactttgt tctgctggag
     1441 aacttcacca gtgaggctgc cttcattgag aacctccggc gtcgattccg ggagaacctc
     1501 atttatacct acattggccc tgtcctggtc tctgtcaacc cctaccggga cctacagatc
     1561 tacacacggc aacatatgga acgctaccgt ggcgtcagtt tctatgaagt gccgcctcat
     1621 ctgtttgcag tggctgacac tgtataccgg gcgcttcgta ctgagcgtcg ggaccaagca
     1681 gtgatgattt ctggagagag tggggcaggg aagacagagg ccaccaagag attgctccag
     1741 ttctatgcag agacctgccc agcccctgaa aggggtggcg cagtgcgaga ccgcctgttg
     1801 cagagcaacc ctgtgctgga ggcctttggg aatgccaaga ctctccgcaa cgacaactcc
     1861 agccgctttg ggaagtacat ggatgtgcag tttgacttca agggtgcccc tgtgggaggc
     1921 cacattctca gttacctcct ggagaagtct cgagtggtac accagaatca cggagaacgg
     1981 aacttccacg tcttttacca gctcctggag gggggggagg aggaggctct tcgtcgtctg
     2041 ggcttggaac ggaaccccca gagctacttg tacctggtga agggccagtg tgccaaggtc
     2101 tcctccatca atgacaagag tgactggaag gttgtgagga aggcgctgtc agtcattgac
     2161 ttcactgagg atgaagtgga ggacttgcta agcatcgtgg ccagcgtcct acatctgggc
     2221 aatatccact ttgctgctga tgaggacagc aatgcccagg ttactactga gaaccagctc
     2281 aaatatctga ccaggctcct tggtgtggaa ggcacaacac tgagggaagc cctgacccac
     2341 aggaagatca tcgccaaggg ggaagagctc ctgagcccac tgaaccttga acaggctgca
     2401 tatgcaaggg atgctcttgc caaggctgtg tacagccgca ctttcacctg gttggtcaga
     2461 aagatcaata ggtctctggc ctctaaggac gctgagagcc ccagctggcg aagcaccaca
     2521 gttcttgggc tcctggacat ttatggcttc gaagtgtttc agcataacag cttcgagcag
     2581 ttctgcatca actattgcaa tgagaagctg cagcagctct tcattgagct gactctcaag
     2641 tccgagcagg aggaatacga ggcagagggc atcgcgtggg aacctgtcca atacttcaac
     2701 aacaagatca tctgtgacct ggtggaggag aaattcaagg gcatcatctc catcttggat
     2761 gaagagtgcc tgcgtcctgg ggaggccaca gacctgacct tcctggagaa gctggaggac
     2821 acaatcaagc accaccctca cttcctgacg cacaagcttg ctgaccagaa gaccaggaaa
     2881 tccctcgacc gaggggagtt ccgccttctt cattatgcgg gagaggtgac ctacagcgtg
     2941 actgggtttc tggataaaaa caatgacctc ctcttccgga acctgaagga gaccatgtgc
     3001 agctccacga accccatcat ggcccagtgc tttgacaaga gtgagctcag tgacaagaag
     3061 cggccagaga cggtggccac ccagttcaag atgagcctct tgcagctagt ggagatcctg
     3121 aggtctaagg aacctgctta tatccggtgc atcaagccaa atgacgccaa acagccgggt
     3181 cgctttgatg aggtgctcat ccgacaccaa gtgaagtacc tgggacttat ggagaatctg
     3241 cgtgtgcgca gagctggctt tgcctaccgt cgaaaatatg aggctttcct gcagaggtac
     3301 aagtcactgt gcccagagac atggcccgtg tggacaggac ggccccagga tggggtggct
     3361 gtgttggtca gacaccttgg ctataagcca gaagagtaca aaatgggcag gactaagatc
     3421 ttcatccgat tccccaagac cttgtttgcc acagaggact ccctggaagt ccgacggcag
     3481 agtctagcca ccaagatcca ggcggcgtgg aggggctttc attggcggca gaaatttctc
     3541 cgggtgaagc gatcagccat ctgtatccag tcctggtggc gcggtacact gggccggagg
     3601 aaggcagcca aaaggaagtg ggcagcccag actatccgta gactcatccg tggcttcatt
     3661 ttgcgtcatg caccccggtg ccctgagaat gccttcttcc tggaccacgt gcgcacgtca
     3721 tttttgctta acctgaggcg acaactaccc cggaatgttc tggacacctc ctggcccaca
     3781 cccccacctg ccctgagaga ggcctcggag ctgctccggg agctatgcat gaagaacatg
     3841 gtgtggaaat actgccggag tatcagccct gagtggaagc agcagctgca gcaaaaggcg
     3901 gtggctagtg aaattttcaa gggcaagaag gacaattacc cccagagtgt ccccagactt
     3961 ttcatcagca cacggcttgg cacagaggag atcagcccca gagtgcttca ggccttgggc
     4021 tctgaaccca tccagtatgc agtgcctgtg gtaaaatacg accgtaaggg ctacaagcct
     4081 cgttcccggc agctgctgct cacacccagt gctgtggtca tcgtggagga tgccaaagtc
     4141 aagcagagaa ttgattatgc caacctaact ggaatctccg tcagtagcct gagtgatagc
     4201 ctgtttgtgc ttcacgtgca gcgtgaagac agcaagcaga agggagatgt ggtactgcag
     4261 agcgatcatg tgatcgagac actaaccaag acggccctca gtgctgaccg tgtgaataat
     4321 atcaacatca accagggcag catcacgttt gcagggggtc caggcaggga tggcatcatc
     4381 gacttcacat ctggctcaga gcttctcatc accaaggcta agaatggcca cctggctgtg
     4441 gtggccccac ggctgaattc tcggtgatga aggtgccggt ggacccctcc tgactcctga
     4501 tgctttgctt agtcccctcc tcccctccca gttaccaaag actcaagctt ccagacaggg
     4561 atccagggac accctcaaag cccacctgca gactcctgcc ccctgctcat cctctcttga
     4621 ggtgatcagg aaccagggag cttccccagg agtgggccag gccgggccaa gcaatagaaa
     4681 agcagaggtc tgagcaggcc aggccagccc tctgctgatg ccaaatatct aagagaaggg
     4741 aattttaact gaggttttct ctgagacttt ttgatgcttt ataggaaact atttttttaa
     4801 aaaagtcatt ttcctatcct aaacacactg gatgtgtttt ccctgactca aacaggacaa
     4861 ggaatgtagc tgaaagactg actgggctgg gctgaggccc cccctctctg gccaagcctc
     4921 tcctcattcc cttggcacct tgtctgtctg tccatccacc ctcgtgccga attcctgcag
     4981 cccgggggat ccactagttc tagataactg atcataatca gccataccac atttgtagag
     5041 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     5101 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     5161 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     5221 ctcatcaatg tatcttaacg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt
     5281 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     5341 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     5401 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     5461 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     5521 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     5581 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     5641 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     5701 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     5761 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     5821 aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
     5881 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     5941 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     6001 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     6061 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     6121 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     6181 ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     6241 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     6301 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     6361 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg
     6421 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     6481 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     6541 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     6601 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     6661 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     6721 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt
     6781 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     6841 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     6901 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     6961 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     7021 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     7081 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     7141 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg
     7201 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa
     7261 agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
     7321 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct
     7381 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
     7441 ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa
     7501 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     7561 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     7621 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     7681 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     7741 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     7801 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     7861 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     7921 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     7981 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     8041 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     8101 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     8161 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     8221 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     8281 cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcat
//
