LOCUS       sequence-267841-        7633 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human  Myo 19  in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7633)
  AUTHORS   Oeding SJ, Majstrowicz K, Hu XP, Schwarz V, Freitag A, Honnert U, 
            Nikolaus P, Bahler M
  TITLE     Identification of Miro1 and Miro2 as mitochondrial receptors for 
            myosin XIX.
  JOURNAL   J Cell Sci. 2018 Sep 10;131(17). pii: jcs.219469. doi: 
            10.1242/jcs.219469.
  PUBMED    30111583
REFERENCE   2  (bases 1 to 7633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2018-09-10"; volume: "131(17). pii: jcs.219469. doi"; 
            pages: " 10.1242/jcs.219469"
COMMENT     Sequence Label: Myo 19-GFP
FEATURES             Location/Qualifiers
     source          1..7633
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      3573..3582
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3579..3581,3582..3584,3585..4298)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3579..3581,3582..3584,3585..4295)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3624..3645)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3885..3904)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4232..4253
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4421..4542
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4458..4477)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4512..4531
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4549..5004)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4686..4707)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4898..4917
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5031..5135
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5137..5494
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5137..5328
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5160..5180)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5345..5480
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5529..6323
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5529..6323
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5583..5602)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6193..6212
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6511..6530)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6555..6602
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6931..7519
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7420..7439
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctgcaatg ctccagcagg tcaatggcca caatccgggg tctgatggcc
      661 aagccaggga gtacctcaga gaagacctgc aggagttcct tggtggggag gtcctgctgt
      721 acaaactgga tgacctcacc agggtgaatc ctgtgacact agagacagtc ctgaggtgcc
      781 tgcaggcccg gtacatggca gacacattct acaccaatgc tggctgcacc ctggtagcct
      841 tgaacccctt caagcctgtt cctcagctct actcgcccga gctaatgaga gagtaccatg
      901 ctgcgcctca gccccagaaa ctgaagcccc atgtgttcac tgtgggtgaa cagacctaca
      961 ggaatgtcaa gagcctgatt gaaccagtca accagtctat tgttgtcagt ggagagagtg
     1021 gtgctggaaa gacatggacg tctcgctgcc taatgaagtt ctatgctgtg gtggccacct
     1081 cacctgcatc ttgggagagc cacaagattg cagagaggat agaacagagg atcctgaact
     1141 ccaaccctgt catggaagct tttgggaatg cgtgtacact gaggaataac aacagcagtc
     1201 gctttgggaa gttcatccag ctccagctga acagggctca gcaaatgact ggagccgcag
     1261 tccagaccta cctcctagag aaaactcgag tggcctgcca ggcttccagt gagaggaact
     1321 tccacatctt ctatcagatt tgcaaaggag ccagtgagga cgagaggctc cagtggcacc
     1381 ttcctgaggg agctgccttc tcctggctgc ccaacccaga gaggagctca gaagaggatt
     1441 gttttgaggt gaccagagag gccatgctcc atttgggcat tgacacccct acccagaaca
     1501 acatctttaa ggtcctagct ggactgctgc accttggcaa tatccagttt gctgcctccg
     1561 aggatgaagc ccagccctgc cagccgatgg atgatgccaa gtactctgtc aggacggcag
     1621 cctcgctgct ggggctccca gaggacgtgc tgctggagat ggtgcagatt agaaccatca
     1681 gggcaggcag acagcagcag gtgttccgga agccctgcgc ccgagccgag tgtgacaccc
     1741 gtagagactg cctggccaaa ctgatctatg cgcggttgtt tgactggctg gtatcagtga
     1801 tcaacagcag catctgtgca gacaccgact cgtggaccac tttcataggc ctgctggatg
     1861 tgtatggatt tgaatcattt cctgacaaca gtctggaaca gttgtgcatc aactacgcca
     1921 atgagaagct gcagcagcat tttgtggctc actacctaag ggcccagcag gaggaatacg
     1981 cagttgaggg cctggagtgg tcattcatca actaccagga caaccagccc tgtttggatc
     2041 tcattgaggg aagccccatc agcatctgct ccctcataaa tgaggaatgc cgcctcaatc
     2101 gacccagcag cgcagcccag ctccagacac gcattgagac tgccctggca ggcagcccct
     2161 gcctgggcca caataagctc agccgggagc ccagcttcat tgtggtgcat tatgcggggc
     2221 ctgtgcggta ccacacagca ggcctggtgg agaagaacaa ggaccctatc ccacctgagc
     2281 tgaccaggct cctgcagcaa tcccaggacc ccctgctcat ggggctgttt cctactaacc
     2341 ccaaagagaa gacccaggag gaaccccctg gccagagcag ggcccctgtg ttgaccgtgg
     2401 tgtccaagtt caaggcctca ctggagcagc ttctgcaggt cctacacagc accacgcccc
     2461 actacattcg ctgcatcaag cccaacagcc agggccaggc gcagaccttt ctccaagagg
     2521 aggtcctgag ccagctggag gcctgtggcc tcgtggagac catccatatc agtgctgctg
     2581 gcttccccat ccgggtctct caccgaaact ttgtagaacg atacaagtta ctaagaaggc
     2641 ttcatccttg cacatcctct ggccccgaca gcccatatcc tgccaaaggg ctccctgaat
     2701 ggtgtccaca cagcgaggaa gccacgcttg aacctctcat ccaggacatt ctccacactc
     2761 tgccggtcct aactcaggca gcagccataa ctggtgactc ggctgaggcc atgccagccc
     2821 ccatgcactg tggcaggacc aaggtgttca tgactgactc tatgctggag cttctggaat
     2881 gtgggcgtgc ccgggtgctg gagcagtgtg cccgctgcat ccagggtggc tggaggcgac
     2941 accggcaccg agagcaggag cggcagtggc gggccgtcat gctcatccag gcagccattc
     3001 gttcctggtt aactcggaaa cacatccaga ggctgcatgc agctgccaca gtcatcaagc
     3061 gtgcatggca gaagtggaga atcagaatgg cctgccttgc tgctaaagag ctggatggtg
     3121 tggaagaaaa acacttctct caagctccct gttccctgag cacctcgccg ctgcagacca
     3181 ggctcctgga ggcaataatc cgcttctggc ccctgggact ggtcctggcc aatacggcta
     3241 tgggtgtagg cagctttcag aggaaattag tggtctgggc ttgcctccag ctccccaggg
     3301 gcagccccag tagctacact gtccagacag cacaagacca ggctggtgtc acgtccatcc
     3361 gagcgctgcc tcagggatcg ataaagtttc actgcagaaa gtctccactg cggtatgctg
     3421 acatctgccc tgaaccttca ccctacagca ttacaggctt taatcagatt ctgctggaaa
     3481 gacacaggct gatccacgtg acctcttctg ccttcactgg gctggggcga attctgcagt
     3541 cgacggtacc gcgggcccgg gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc
     3601 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     3661 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     3721 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
     3781 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     3841 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     3901 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     3961 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     4021 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     4081 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     4141 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     4201 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     4261 ccgggatcac tctcggcatg gacgagctgt acaagtaaag cggccgcgac tctagatcat
     4321 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     4381 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     4441 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     4501 gcattctagt tgtggtttgt ccaaactcat caatgtatct taaggcgtaa attgtaagcg
     4561 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     4621 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     4681 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     4741 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     4801 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     4861 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     4921 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     4981 ttaatgcgcc gctacagggc gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     5041 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     5101 gataaatgct tcaataatat tgaaaaagga agagtcctga ggcggaaaga accagctgtg
     5161 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     5221 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     5281 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     5341 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     5401 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     5461 aggaggcttt tttggaggcc taggcttttg caaagatcga tcaagagaca ggatgaggat
     5521 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga
     5581 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc
     5641 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga
     5701 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg
     5761 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc
     5821 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg
     5881 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga
     5941 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc
     6001 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca
     6061 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg
     6121 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct
     6181 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg
     6241 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc
     6301 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac
     6361 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt
     6421 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga
     6481 gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa taccggaagg
     6541 aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt
     6601 tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac cgagacccca
     6661 ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa gttcgggtga
     6721 aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct caggttactc
     6781 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     6841 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     6901 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     6961 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     7021 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     7081 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     7141 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     7201 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     7261 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     7321 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     7381 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     7441 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     7501 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     7561 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     7621 taccgccatg cat
//
