LOCUS       sequence-267842-        7620 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human  Myo 19  in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7620)
  AUTHORS   Oeding SJ, Majstrowicz K, Hu XP, Schwarz V, Freitag A, Honnert U, 
            Nikolaus P, Bahler M
  TITLE     Identification of Miro1 and Miro2 as mitochondrial receptors for 
            myosin XIX.
  JOURNAL   J Cell Sci. 2018 Sep 10;131(17). pii: jcs.219469. doi: 
            10.1242/jcs.219469.
  PUBMED    30111583
REFERENCE   2  (bases 1 to 7620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2018-09-10"; volume: "131(17). pii: jcs.219469. doi"; 
            pages: " 10.1242/jcs.219469"
COMMENT     Sequence Label: GFP-Myo 19
FEATURES             Location/Qualifiers
     source          1..7620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      607..616
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(613..615,616..618,619..1329)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(658..679)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(919..938)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1266..1287
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4408..4529
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4445..4464)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4499..4518
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(4536..4991)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4673..4694)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4885..4904
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5018..5122
                     /gene="bla"
                     /label=AmpR promoter
     promoter        5124..5481
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5124..5315
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5147..5167)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5332..5467
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5516..6310
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5516..6310
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5570..5589)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6180..6199
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6498..6517)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6542..6589
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      6918..7506
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7407..7426
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
      841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1321 ctgtacaagt ccggactcag atctgcaatg ctccagcagg tcaatggcca caatccgggg
     1381 tctgatggcc aagccaggga gtacctcaga gaagacctgc aggagttcct tggtggggag
     1441 gtcctgctgt acaaactgga tgacctcacc agggtgaatc ctgtgacact agagacagtc
     1501 ctgaggtgcc tgcaggcccg gtacatggca gacacattct acaccaatgc tggctgcacc
     1561 ctggtagcct tgaacccctt caagcctgtt cctcagctct actcgcccga gctaatgaga
     1621 gagtaccatg ctgcgcctca gccccagaaa ctgaagcccc atgtgttcac tgtgggtgaa
     1681 cagacctaca ggaatgtcaa gagcctgatt gaaccagtca accagtctat tgttgtcagt
     1741 ggagagagtg gtgctggaaa gacatggacg tctcgctgcc taatgaagtt ctatgctgtg
     1801 gtggccacct cacctgcatc ttgggagagc cacaagattg cagagaggat agaacagagg
     1861 atcctgaact ccaaccctgt catggaagct tttgggaatg cgtgtacact gaggaataac
     1921 aacagcagtc gctttgggaa gttcatccag ctccagctga acagggctca gcaaatgact
     1981 ggagccgcag tccagaccta cctcctagag aaaactcgag tggcctgcca ggcttccagt
     2041 gagaggaact tccacatctt ctatcagatt tgcaaaggag ccagtgagga cgagaggctc
     2101 cagtggcacc ttcctgaggg agctgccttc tcctggctgc ccaacccaga gaggagctca
     2161 gaagaggatt gttttgaggt gaccagagag gccatgctcc atttgggcat tgacacccct
     2221 acccagaaca acatctttaa ggtcctagct ggactgctgc accttggcaa tatccagttt
     2281 gctgcctccg aggatgaagc ccagccctgc cagccgatgg atgatgccaa gtactctgtc
     2341 aggacggcag cctcgctgct ggggctccca gaggacgtgc tgctggagat ggtgcagatt
     2401 agaaccatca gggcaggcag acagcagcag gtgttccgga agccctgcgc ccgagccgag
     2461 tgtgacaccc gtagagactg cctggccaaa ctgatctatg cgcggttgtt tgactggctg
     2521 gtatcagtga tcaacagcag catctgtgca gacaccgact cgtggaccac tttcataggc
     2581 ctgctggatg tgtatggatt tgaatcattt cctgacaaca gtctggaaca gttgtgcatc
     2641 aactacgcca atgagaagct gcagcagcat tttgtggctc actacctaag ggcccagcag
     2701 gaggaatacg cagttgaggg cctggagtgg tcattcatca actaccagga caaccagccc
     2761 tgtttggatc tcattgaggg aagccccatc agcatctgct ccctcataaa tgaggaatgc
     2821 cgcctcaatc gacccagcag cgcagcccag ctccagacac gcattgagac tgccctggca
     2881 ggcagcccct gcctgggcca caataagctc agccgggagc ccagcttcat tgtggtgcat
     2941 tatgcggggc ctgtgcggta ccacacagca ggcctggtgg agaagaacaa ggaccctatc
     3001 ccacctgagc tgaccaggct cctgcagcaa tcccaggacc ccctgctcat ggggctgttt
     3061 cctactaacc ccaaagagaa gacccaggag gaaccccctg gccagagcag ggcccctgtg
     3121 ttgaccgtgg tgtccaagtt caaggcctca ctggagcagc ttctgcaggt cctacacagc
     3181 accacgcccc actacattcg ctgcatcaag cccaacagcc agggccaggc gcagaccttt
     3241 ctccaagagg aggtcctgag ccagctggag gcctgtggcc tcgtggagac catccatatc
     3301 agtgctgctg gcttccccat ccgggtctct caccgaaact ttgtagaacg atacaagtta
     3361 ctaagaaggc ttcatccttg cacatcctct ggccccgaca gcccatatcc tgccaaaggg
     3421 ctccctgaat ggtgtccaca cagcgaggaa gccacgcttg aacctctcat ccaggacatt
     3481 ctccacactc tgccggtcct aactcaggca gcagccataa ctggtgactc ggctgaggcc
     3541 atgccagccc ccatgcactg tggcaggacc aaggtgttca tgactgactc tatgctggag
     3601 cttctggaat gtgggcgtgc ccgggtgctg gagcagtgtg cccgctgcat ccagggtggc
     3661 tggaggcgac accggcaccg agagcaggag cggcagtggc gggccgtcat gctcatccag
     3721 gcagccattc gttcctggtt aactcggaaa cacatccaga ggctgcatgc agctgccaca
     3781 gtcatcaagc gtgcatggca gaagtggaga atcagaatgg cctgccttgc tgctaaagag
     3841 ctggatggtg tggaagaaaa acacttctct caagctccct gttccctgag cacctcgccg
     3901 ctgcagacca ggctcctgga ggcaataatc cgcttctggc ccctgggact ggtcctggcc
     3961 aatacggcta tgggtgtagg cagctttcag aggaaattag tggtctgggc ttgcctccag
     4021 ctccccaggg gcagccccag tagctacact gtccagacag cacaagacca ggctggtgtc
     4081 acgtccatcc gagcgctgcc tcagggatcg ataaagtttc actgcagaaa gtctccactg
     4141 cggtatgctg acatctgccc tgaaccttca ccctacagca ttacaggctt taatcagatt
     4201 ctgctggaaa gacacaggct gatccacgtg acctcttctg ccttcactgg gctggggtga
     4261 tctagagaca tccatatgac tagtagatcc tctagataac tgatcataat cagccatacc
     4321 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     4381 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4441 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4501 ggtttgtcca aactcatcaa tgtatcttaa cgcgtaaatt gtaagcgtta atattttgtt
     4561 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     4621 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     4681 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     4741 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     4801 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     4861 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     4921 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     4981 acagggcgcg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     5041 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     5101 ataatattga aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag
     5161 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     5221 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     5281 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     5341 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     5401 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     5461 ggaggcctag gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat
     5521 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     5581 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     5641 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga
     5701 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     5761 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     5821 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     5881 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     5941 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     6001 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga
     6061 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     6121 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     6181 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     6241 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     6301 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     6361 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     6421 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     6481 cctaggggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg
     6541 acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg
     6601 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac
     6661 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc
     6721 gcagccaacg tcggggcggc aggccctgcc atagcctcag gttactcata tatactttag
     6781 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     6841 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     6901 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     6961 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     7021 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     7081 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     7141 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     7201 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     7261 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     7321 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     7381 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     7441 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     7501 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     7561 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat
//
