LOCUS       sequence-267872-       10374 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression and purification of c.elegans Myo 9 (Hum 7) using 
            baculovirus from pFastBac1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10374)
  AUTHORS   Liao W, Elfrink K, Bahler M
  TITLE     Head of myosin IX binds calmodulin and moves processively toward the
            plus-end of actin filaments.
  JOURNAL   J Biol Chem. 2010 Aug 6;285(32):24933-42. doi: 
            10.1074/jbc.M110.101105. Epub 2010 Jun 10.
  PUBMED    20538589
REFERENCE   2  (bases 1 to 10374)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M110.101105"; journalName: "J Biol Chem"; date: 
            "2010-08-6- 6"; volume: "285"; issue: "32"; pages: "24933-42"
COMMENT     Sequence Label: Myo 9 Avi+Flag
FEATURES             Location/Qualifiers
     source          1..10374
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      2..456
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(89..108)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     298..319
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        482..586
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(587..655,656..1447)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(805..824)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1618..2206
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2107..2126
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2360..2377
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  2511..2735
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2802..3335)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3524..3529,3530..3546,3547..3552))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        3904..3995
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     3920..3937
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             9648..9692
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             9702..9725
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    9864..9998
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9901..9920)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9955..9974
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  10027..10192
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt cttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
      481 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
      541 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
      601 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
      661 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
      721 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
      781 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
      841 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
      901 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
      961 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     1021 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     1081 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     1141 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     1201 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     1261 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     1321 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     1381 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     1441 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     1501 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     1561 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     1621 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     1681 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     1741 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     1801 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     1861 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     1921 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     1981 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     2041 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     2101 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     2161 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     2221 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     2281 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     2341 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg
     2401 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atagaccagc cgcgtaacct
     2461 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga
     2521 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag
     2581 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt
     2641 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga
     2701 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac
     2761 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg
     2821 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg
     2881 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca
     2941 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact
     3001 gcgagatcat agatatagat ctcactacgc ggctgctcaa acttgggcag aacgtaagcc
     3061 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta
     3121 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct
     3181 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg
     3241 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg
     3301 ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca
     3361 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa
     3421 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     3481 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca
     3541 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     3601 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc
     3661 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg
     3721 cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt
     3781 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt
     3841 gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa
     3901 tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
     3961 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
     4021 ccatcgggcg cggatccatg tcatttgact caatatcagc tggagcgacg ggatgttttc
     4081 cccgacgtgc tcacacaatc aacgcgacag acgatgcaca cacacatacg gtagccgtct
     4141 atatgcacag cttcaatccc gactgtgaaa atgatacggt acacttggag gttcataagc
     4201 ggtgcacgac ggaggagctc atcgaaaaag tgctcgccgg gagagctgaa ctagaaggcc
     4261 agtcggtaca agactttgac ctcttcgaaa tgatgggcac ccccgacggt caaacctata
     4321 aagaacgccg cctagacccc ggggaatacc cagtcgcggt acaggccatc tggtcaagac
     4381 tgcctgtagt ggtggacagt gccactccaa aaaatcgatt tgtttttcga caccgcggtt
     4441 atcgggcggc aaccggcggc actcggtttg gaagtgcaga ggtcgcctca tcgattgacg
     4501 cttttcttac gaaattcttg gttcagccac aggaccgcga gtacgccgat ctctgtaact
     4561 tgccagagct cacggagcaa accctcttgg acaatttgcg ggaaagattc gcgaatgggc
     4621 acatttacac gtacatcggt ccaattctag tagccgtgaa cccgttttgc ttttttccga
     4681 tctacaaccc aaaatatgct aggctatact ttcaaagtaa gcggctcggc tcactaccac
     4741 cgcacatttt tgcaatcgcc gatgtctgtt accacaacat gctccgtatc aaggaaaatc
     4801 aatgcgtggt aatctccggg gaaagtggtt cgggaaaaac ggaatccacg aatcatctga
     4861 tgtcccattt gatatcgcta tcacagaaag gttcaacagg ctgcagtact gagcagacac
     4921 ttctgtcagc tggtcctgtt ctagaggctt ttggaaatgc tgtcacatta acgaacaata
     4981 atagtagcag atttggaaaa ttcatcaaaa tcaactatcg ggagaatgga atggtttccg
     5041 gagcaaacgt ggagatctac ctgctggaaa aatcacgcat catatttcaa actaaaggag
     5101 aacgaaacta ccacgtattc tactaccttc tggagggtgc agacgaggaa gaaaggaaga
     5161 aatatttctt gttgaagcca catgattata agtatttgaa tcagaacgag ccatttgcac
     5221 tggaaggtgt gaacgagcgg aatgagtttg ataggttgag gcatgcaatg agctccgtcg
     5281 gattttgtgc aaagacccag cagaccatct tcgggattat ttcggcagtg ttgcttcttg
     5341 gcaatatcac atatatcaag agacatggct accatagcga tgaaagtggg tatattgaaa
     5401 atgaagaagt cgtggattta gtcgcaaatc tgcttcacat caaaaccgac actctcatgc
     5461 aggctctcac tatgaaacga catgttatga agacagaaac tgtagttttg cgatatagtg
     5521 tctctgaggc caccaacacc cgagatgcca tggcaaagtg catatacaac tctttgtttc
     5581 actatatagt cctcaggatt aatcaggcat tgctgaagaa ggacttctca gctgggaagg
     5641 gatattatat aggtatcttg gatatattcg gatttgaaga cgttgggtct caatgcaaca
     5701 gctttgaaca gttatgtatc aactatgcca atgagaaact tcagtcctat ttcaatcaac
     5761 atattttcca atttgaacaa gaggagtact tgaaggaggg catttcttgg accaatatcg
     5821 agtatactga taatacggag tgtgtgcagc tgtttcagag taaaccatat ggaattctca
     5881 ggctagtcga tgaggagagt aatatcaata atggaacaga tgactcaatg ctggcgaagt
     5941 taaatcagtt tttgaagaac aacgaatact atgagacccc acagaagaag gagccggcat
     6001 tcatcgtggc tcattatgct ggaaaagtca agtatcaaat tacgggtttc cgagaaaaga
     6061 ataaggacct aatgagacag gatgtgttga acgctctgaa gtccagtacg agctcagtta
     6121 tgaagaccct gctgggaatc gatccggtgg cggtgcacag gtggaatgtg ctgagaagtg
     6181 tgtttcgggc gatgaacgcg ttcaagcaat cgacaaggaa actgcagaaa agtgaatccg
     6241 ccggccatct ccgagtaatg gactccatcg taacgtctcc ccgtcgtggc tcggactctg
     6301 ctctctccgc atttctacgt ggcgaacttc gctgtgaagt tccagacttt tgtgatacca
     6361 gcatgttcaa cacgattaga aatcaagcac ggcgtacccc ggcgggaaag tctgacgaca
     6421 aaatgagctt gctgaaatcg ctgcaaattc tgaaggaggc tatcggagga cggaggcttg
     6481 ccaagaagcc atcgtcggtt tcaaagcagt tcgagtactc gttgacgagg ttgatgagca
     6541 cactggccaa cgcgacgccg tattttataa ggtgtattaa gagtaataat gataagatcg
     6601 ccaaccactt cgacgacaac ataatcctcc gccagctccg ctacaccgga atgctcgaga
     6661 ccgttcgaat tcggagagcc gggtactctg tgagaattga gtatccaagc tttgtccagc
     6721 aataccgaat tcttttgcga aacggaaggg atagtactgt ggacgatgtg aaagagtaca
     6781 tacattccca tgcatcgatt gataatgata atattcaata tggaaccaat aagattttta
     6841 tgagagacgc tgaaaagttg atactcgatg atcatttgca tcggacaatt atgcaatcaa
     6901 tcgatacact tcaacgatgg ttcaggacaa tgttggctag aaagcgatat ttgagaatga
     6961 aagagggtat tatcaagatc caagcattga ttcgaggatc atttgcaaga gccgaagttc
     7021 ggaaaaaagc cttagcagct caaacgatac agtgtaattg gaagaagtac aagcaacgac
     7081 agaaatattt atcgataaga gaatcggtga tagctctgca gtctgcgtat cgcggagcat
     7141 cggttcgcaa gcgagtcggc gagattccaa agggcaatgg agccttgaac gttaagaagt
     7201 caccgtttag agttcggaag gttcatgctg tgaatcttac aaagttcgac ttgagtgatc
     7261 cgagctctct ggcggctttt gctctttcgg atgatgattc ggatagtgat aagtctacgc
     7321 aggatgggct tgatgatgat atttcggaag agagtgatgc ttttcaggac gatgacgccg
     7381 tcgacgtgga cgccacattt atcctggaag acacaaagtt gaagctcatt gaaggagctg
     7441 atctatccca tcatagacgg caatctctgg cccctacggc ttcaaccacc aaattaaaaa
     7501 tgttacggag ggctaatagt accgaatcca acaatttctc aatgtcaaga tgtgatgact
     7561 cgttcacgct aagcccaaca cattccaaaa agaaaggagg cgctggaaag ttgggattcc
     7621 agaaggcaaa aaagaatttg aaggcacttt ttggcaggaa cgaggacgag gatgaaccgt
     7681 ctccgggacc aagttttgcc agtgaactga cgccggacgt gttcgtcgca aaagaccatc
     7741 aattcaagat gtcacgactt catcggcagg agatatgtgc tctttgcaat cggcacctga
     7801 cgaacttcat ctcacaggcg cacaagtgtc agagatgcaa aatgtgcctg cacaaggaat
     7861 gcttcgtctt cgcctcctcc atcccgtgca atcccacgtc accagttcga tcaccaactc
     7921 ggctgcattc accgaaacgg ccatgggatt tgatgcccca caagcagcgg caatcctcct
     7981 caccaattcc atcgaccggc acgttcagtt taacaaaaac caagcaacag atcgaccctg
     8041 gaaacatgct tgtagaatcc accgacgatc tccgacaatt cagtattttt attttcaaca
     8101 agacacgtca tctaaacgag agtaatgcca aaagggatac tgtagcggat gcggtcttca
     8161 aaaagtcgct aagagctttc catatggagc tgttgggata tgaggcggtt ttatcggtcg
     8221 agcaatccgt gcttaaatac cgtgacgtca tcacaatgtt cgagggactt ttaacgaaag
     8281 tctgtctcga ggagagtgtg tcctttccga caacacttgg cgttaacgct tttagaggtt
     8341 tcctgaacga atacgttcat gtgcaatcga aaaagaagcg cgggaaggag aagtcatcaa
     8401 tgataaaggt acatgctggt caccgattcc gtgccgaagc agtccatgtg ccgacctatt
     8461 gtgaagtgtg taatcagtta atatggcacc atgagaagct gtacacttgt gttgcgtgcc
     8521 ggatatcgtg tcataaaaag tgtcagccga aggtcacaca tccgtgtcag atgactggaa
     8581 aggcgattga tccgaagaca aatggtggaa gattctttgg cgcctcttta gtatcaattg
     8641 tcgatgatga tcatactgtg ccaacactct tggaccgact cttttttgca attgagacac
     8701 gtgccctgtt tgtggaagga gtttatagaa aaagtggatc tctaccccaa gttcgaagta
     8761 tcagaaaagt gattgaatcg actgcagatg cggattctgt gaatcttgag gatattggag
     8821 ttcatgtgct aactacactt gtcaaggcgt tcttccgaga actcgccgaa ccaattatca
     8881 tttttgatct ctatgaaaat tttttgaatg tttcagaagt ggaggatatg ggtgaacgtg
     8941 tccgttgtct ttccgtaatg atcgagcttc tcccaaaacc gaaccgcgcc gtgctcgatc
     9001 gcctaatgta tcatttggca cgtgtcgccg accaggaagc cgtcaacaaa atgggctgca
     9061 acaacttggc tctgatcttc gggccatgtg tgctccgccg acaagatagt gctcatgctc
     9121 aggagcagtt gaatgacgtg gcacggcaaa cgggttgtgt tcagacttta attgaggaga
     9181 agctgaaaca gtataaagcc accattcata acattgttga gctggaggat gctagtcaga
     9241 aggtatcagc aaacttgcga aaaattgaag agcaccgtcg gaatagtgaa ccatcgaaat
     9301 tttcgccgaa tattgggact gcaaagcaat tgttcgagga acaactcgaa ttcttgggaa
     9361 agcaaaaaga acgcctttta caagagctcc caccactggc tccggtcgcc tcctcagaag
     9421 atctttcctc atccgacgag aacgcaatcc acgcgtcaag ctccctttcc gtcgaggagt
     9481 acgcacttga tctcgacgca ccgcccgtat tttgtctgct gcgctatccg actaagctcc
     9541 gcccaccggg tcccgcccat cgacgacgtc ctgacttgga tcttcggaga aaagcgttcg
     9601 ccaactcgcg tggcatcgtc tcattttatt caccggtcgc ggccgccggt ctgaacgaca
     9661 tcttcgaggc tcagaaaatc gaatggcacg aaggtggcgg tgactacaag gacgacgatg
     9721 acaagtgagg taccaagctt gtcgagaagt actagaggat cataatcagc cataccacat
     9781 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     9841 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     9901 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     9961 tgtccaaact catcaatgta tcttatcatg tctggatctg atcactgctt gagcctagga
    10021 gatccgaacc agataagtga aatctagttc caaactattt tgtcattttt aattttcgta
    10081 ttagcttacg acgctacacc cagttcccat ctattttgtc actcttccct aaataatcct
    10141 taaaaactcc atttccaccc ctcccagttc ccaactattt tgtccgccca cagcggggca
    10201 tttttcttcc tgttatgttt ttaatcaaac atcctgccaa ctccatgtga caaaccgtca
    10261 tcttcggcta ctttttctct gtcacagaat gaaaattttt ctgtcatctc ttcgttatta
    10321 atgtttgtaa ttgactgaat atcaacgctt atttgcagcc tgaatggcga atgg
//
