LOCUS       sequence-267874-        8914 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses XRCC2_+_152373143.23-P1P2 sgRNA in pCR1068.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8914)
  AUTHORS   Richardson CD, Kazane KR, Feng SJ, Zelin E, Bray NL, Schafer AJ, 
            Floor SN, Corn JE
  TITLE     CRISPR-Cas9 genome editing in human cells occurs via the Fanconi 
            anemia pathway.
  JOURNAL   Nat Genet. 2018 Aug;50(8):1132-1139. doi: 10.1038/s41588-018-0174-0.
            Epub 2018 Jul 27.
  PUBMED    30054595
REFERENCE   2  (bases 1 to 8914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41588-018-0174-0"; journalName: "Nat Genet"; date: 
            "2018-08"; volume: "50"; issue: "8"; pages: "1132-1139"
COMMENT     Sequence Label: pCR1279
FEATURES             Location/Qualifiers
     source          1..8914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(189..387)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(214..234)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        389..768
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     940..959
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        1034..1138
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1139..1207,1208..1999)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1357..1376)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2170..2758
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2659..2678
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             2820..3000
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     3509..3525
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3510..3526
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3529..4117
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3635..3646
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     4044..4064
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    4173..4206
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(join(4226..4939,4940..4942,4943..4945))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4271..4292)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4620..4639
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4879..4900
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4942..4951
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(4958..5011)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(5021..5617)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5101..5121)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     5598..5617
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     intron          5638..6576
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(5661..5681)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        complement(6577..6788)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     primer_bind     complement(7041..7061)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     protein_bind    7065..7098
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    7359..7476
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(7774..7818)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8003..8236
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    8733..8858
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             join(8905..8914,1..171)
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 tttccacact gactaaaagg gtctgaggga tctctagtta ccagagtcac acaacagacg
       61 ggcacacact acttgaagca ctcaaggcaa gctttattga ggcttaagca gtgggttccc
      121 tagttagcca gagagctccc aggctcagat ctggtctaac cagagagacc cagtacaggc
      181 aaaacgcgct gcttatatag acctcccacc gtacacgcct accgcccatt tgcgtcaatg
      241 gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa aacaaactcc
      301 cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc tatccacgcc
      361 cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat acgtagatgt
      421 actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag gcgggccatt
      481 taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga tgtactgcca
      541 agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc cctattggcg
      601 ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg ggcggtcagc
      661 caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg ctatgaacta
      721 atgaccccgt aattgattac tattaataac tagtcaataa tcaatgtcaa cgcgtatatc
      781 tggcccgtac atcgcgaagc agcgcaaaac gcctaaccct aagcagattc ttcatgcaat
      841 tgtcggtcaa gccttgcctt gttgtagctt aaattttgct cgcgcactac tcagcgacct
      901 ccaacacaca agcagggagc agatactggc ttaactatgc ggcatcagag cagattgtac
      961 tgagagtgca ccatagggga tcgggagatc tcccgatccg tcgacgtcag gtggcacttt
     1021 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     1081 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     1141 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     1201 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     1261 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     1321 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     1381 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     1441 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     1501 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     1561 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     1621 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     1681 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     1741 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     1801 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     1861 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     1921 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     1981 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     2041 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     2101 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     2161 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     2221 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     2281 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     2341 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     2401 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     2461 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     2521 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     2581 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     2641 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     2701 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     2761 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgct
     2821 gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac cagagtcaca
     2881 caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag gcttaagcag
     2941 tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc agagagaccc
     3001 agtacagtcc ggatgcagct ctcgggccat gtgatgaaat gctaggcggc tgtcaaacct
     3061 ccactctaat acttctctct ccgggtcatc catcccatgc aggctcacag ggtgtaacaa
     3121 gcgggtgttc tctccttcat tggcttcttc taccttctct tgctcaactg gtactagctt
     3181 gtagcaccat ccaaaggtca gtggatatct gatccctggc cctggtgtgt agttctgcca
     3241 atcagggaag tagccttgtg tgtggtagat ccacagatca aggatatctt gtcttcgttg
     3301 ggagtgaatt agcccttcca gtcccccctt ttcttttaaa aagtggctaa gatctacagc
     3361 tgccttgtaa gtcattggtc ttaaaggtac ctgaggtgtg actggaaaac ccacctcctc
     3421 ctcctcttgt gcttctagcc aggcacaatc agcattggta gctgctgtat tgctacttgt
     3481 gattgctcca tgtttttcta ggtctcgatc gaggtcgacg gtatcgatgc ggggaggcgg
     3541 cccaaaggga gatccgactc gtctgagggc gaaggcgaag acgcggaaga ggccgcagag
     3601 ccggcagcag gccgcgggaa ggaaggtccg ctggattgag ggccgaaggg acgtagcaga
     3661 aggacgtccc gcgcagaatc caggtggcaa cacaggcgag cagccaagga aaggacgatg
     3721 atttccccga caacaccacg gaattgtcag tgcccaacag ccgagcccct gtccagcagc
     3781 gggcaaggca ggcggcgatg agttccgccg tggcaatagg gagggggaaa gcgaaagtcc
     3841 cggaaaggag ctgacaggtg gtggcaatgc cccaaccagt gggggttgcg tcagcaaaca
     3901 cagtgcacac cacgccacgt tgcctgacaa cgggccacaa ctcctcataa agagacagca
     3961 accaggattt atacaaggag gagaaaatga aagccatacg ggaagcaata gcatgataca
     4021 aaggcattaa agcagcgtat ccacatagcg taaaaggagc aacatagtta agaataccag
     4081 tcaatctttc acaaattttg taatccagag gttgattatc gataagcttg atatcgaatt
     4141 gtacctagtg gaaccggaac ccttaaacat gtataacttc gtataatgta tgctatacga
     4201 agttattagg tccctcgacg aattcttact tgtacagctc gtccatgccg agagtgatcc
     4261 cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttac
     4321 tcagcttgga ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga
     4381 tgggggtgtt ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc
     4441 ggatcttgaa gttcaccttg atgccgttct tctgcttgtc ggccatgata tagacgttgt
     4501 ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga
     4561 tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg
     4621 tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca
     4681 tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg aagcactgca
     4741 cgccatgggt cagggtggtc acgagggtgg gccagggcac cggcagcttg ccggtggtgc
     4801 agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc
     4861 tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga
     4921 acagctcctc gcccttgctc accatggtgg cgaccggtgg gccaggattc tcctcgacgt
     4981 caccgcatgt tagcagactt cctctgccct ctcccgatcc ggcaccgggc ttgcgggtca
     5041 tgcaccaggt gcgcggtcct tcgggcacct cgacgtcggc ggtgacggtg aagccgagcc
     5101 gctcgtagaa ggggaggttg cggggcgcgg aggtctccag gaaggcgggc accccggcgc
     5161 gctcggccgc ctccactccg gggagcacga cggcgctgcc cagacccttg ccctggtggt
     5221 cgggcgagac gccgacggtg gccaggaacc acgcgggctc cttgggccgg tgcggcgcca
     5281 ggaggccttc catctgttgc tgcgcggcca gccgggaacc gctcaactcg gccatgcgcg
     5341 ggccgatctc ggcgaacacc gcccccgctt cgacgctctc cggcgtggtc cagaccgcca
     5401 ccgcggcgcc gtcgtccgcg acccacacct tgccgatgtc gagcccgacg cgcgtgagga
     5461 agagttcttg cagctcggtg acccgctcga tgtggcggtc cgggtcgacg gtgtggcgcg
     5521 tggcggggta gtcggcgaac gcggcggcga gggtgcgtac ggcccggggg acgtcgtcgc
     5581 gggtggcgag gcgcaccgtg ggcttgtact cggtcatggt gggctagctc acgacacctg
     5641 aaatggaaga aaaaaacttt gaaccactgt ctgaggcttg agaatgaacc aagatccaaa
     5701 ctcaaaaagg gcaaattcca aggagaatta catcaagtgc caagctggcc taacttcagt
     5761 ctccacccac tcagtgtggg gaaactccat cgcataaaac ccctcccccc aacctaaaga
     5821 cgacgtactc caaaagcgcg agaactaatc gaggtgcctg gacggcgccc ggtactccgt
     5881 ggagtcacat gaagcgacgg ctgaggacgg aaaggccctt ttcctttgtg tgggtgactc
     5941 acccgcccgc tctcccgagc gccgcgtcct ccattttgag ctccctgcag cagggccggg
     6001 aagcggccat ctttccgctc acgcaactgg tgccgaccgg gccagccttg ccgcccaggg
     6061 cggggcgata cacggcggcg cgaggccagg caccagagca ggccggccag cttgagacta
     6121 cccccgtccg attctcggtg gccgcgctcg caggccccgc ctcgccgaac atgtgcgctg
     6181 ggacgcacgg gccccgtcgc cgcccgcggc cccaaaaacc gaaataccag tgtgcagatc
     6241 ttggcccgca tttacaagac tatcttgcca gaaaaaaagc gtcgcagcag gtcatcaaaa
     6301 attttaaatg gctagagact tatcgaaagc agcgagacag gcgcgaaggt gccaccagat
     6361 tcgcacgcgg cggccccagc gcccaggcca ggcctcaact caagcacgag gcgaaggggc
     6421 tccttaagcg caaggcctcg aactctccca cccacttcca acccgaagct cgggatcaag
     6481 aatcacgtac tgcagccagg tggaagtaat tcaaggcacg caagggccat aacccgtaaa
     6541 gaggccaggc ccgcgggaac cacacacggc acttacctgt gttctggcgg caaacccgtt
     6601 gcgaaaaaga acgttcacgg cgactactgc acttatatac ggttctcccc caccctcggg
     6661 aaaaaggcgg agccagtaca cgacatcact ttcccagttt accccgcgcc accttctcta
     6721 ggcaccggtt caattgccga cccctccccc caacttctcg gggactgtgg gcgatgtgcg
     6781 ctctgcccac tgacgggcac atgcatggcg gtaatacggt tatccaccct gcagggcggc
     6841 cgcctaatgg atcctagtac tcgagaaaaa aagcaccgac tcggtgccac tttttcaagt
     6901 tgataacgga ctagccttat ttaaacttgc tatgctgttt ccagcttagc tcttaaacat
     6961 ggaaggcact acacatccca acaaggtggt tctccaaggg atacttatag tctcaaaaca
     7021 cacaattact ttacagttag ggtgagtttc cttttgtgct gtttataact tcgtataatg
     7081 tatgctatac gaagttatag aaatccaagc ctatcatgta aaatgtagct agtattaaaa
     7141 agaacagatt atctgtcttt tatcgcacat taagcctcta tagttactag gaaatattat
     7201 atgcaaatta accggggcag gggagtagcc gagcttctcc cacaagtccg tgcgaggggg
     7261 ccggcgcggg cctagagatg gcgcgtcgga tctctagacc tgcagggcgg gcccggtact
     7321 aaccaaactg gatctctgct gtccctgtaa taaacccgaa aattttgaat ttttgtaatt
     7381 tgtttttgta attctttagt ttgtatgtct gttgctatta tgtctactat tctttcccct
     7441 gcactgtacc ccccaatccc cccttttctt ttaaaattgt ggatgaatac tgccatttgt
     7501 ctgcagaatt ggcgcacgca gtgccgatcc gttcactaat cgaatggatc tgtctctgtc
     7561 tctctctcca ccttcttctt ctattccttc gggcctgtcg ggtcccctcg gggttgggag
     7621 gtgggtctga aacgataatg gtgaatatcc ctgcctaact ctattcacta tagaaagtac
     7681 agcaaaaact attcttaaac ctaccaagcc tcctactatc attatgaata attttatata
     7741 ccacagccaa tttgttatgt taaaccaatt ccacaaactt gcccatttat ctaattccaa
     7801 taattcttgt tcattctttt cttgctggtt ttgcgattct tcaattaagg agtgtattaa
     7861 gcttgtgtaa ttgttaattt ctctgtccca ctccatccag gtcgtgtgat tccaaatctg
     7921 ttccagagat ttattactcc aactagcatt ccaaggcaca gcagtggtgc aaatgagttt
     7981 tccagagcaa ccccaaatcc ccaggagctg ttgatccttt aggtatcttt ccacagccag
     8041 gattcttgcc tggagctgct tgatgcccca gactgtgagt tgcaacagat gctgttgcgc
     8101 ctcaatagcc ctcagcaaat tgttctgctg ctgcactata ccagacaata attgtctggc
     8161 ctgtaccgtc agcgtcattg acgctgcgcc catagtgctt cctgctgctc ccaagaaccc
     8221 aaggaacaaa gctcctattc ccactgctct tttttctctc tgcaccactc ttctctttgc
     8281 cttggtgggt gctactccta atggttcaat ttttactact ttatatttat ataattcact
     8341 tctccaattg tccctcatat ctcctcctcc aggtctgaag atcagcggcc ggccgcttgc
     8401 tgtgcggtgg tcttactttt gttttgctct tcctctatct tgtctaaagc ttccttggtg
     8461 tcttttatct ctatcctttg atgcacacaa tagagggttg ctactgtatt atataatgat
     8521 ctaagttctt ctgatcctgt ctgaagggat ggttgtagct gtcccagtat ttgtctacag
     8581 ccttctgatg tttctaacag gccaggatta actgcgaatc gttctagctc cctgcttgcc
     8641 catactatat gttttaattt atattttttc tttccccctg gccttaaccg aattttttcc
     8701 catcgcgatc taattctccc ccgcttaata ctgacgctct cgcacccatc tctctccttc
     8761 tagcctccgc tagtcaaaat ttttggcgta ctcaccagtc gccgcccctc gcctcttgcc
     8821 gtgcgcgctt cagcaagccg agtcctgcgt cgagagagct cctctggttt ccctttcgct
     8881 ttcaagtccc tgttcgggcg ccactgctag agat
//
