LOCUS       sequence-267904-       11477 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of AAK1 (human) tagged with C-terminal V5 
            for generating protein-proximity networks using proximity-dependent 
            biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11477)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 11477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-AAK1-V5_IDG-K
FEATURES             Location/Qualifiers
     source          1..11477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        182..408
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             409..589
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    636..761
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1254..1487
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1672..1716
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1983..2099
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2131..2151
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        2151..3362
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3199..3218
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    3363..3387
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6010..6034)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6033..6074
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(6133..6152)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     protein_bind    complement(6269..6289)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    6318..6906
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6371..6391)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6789..6800)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6920..7419
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6955..6978)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7352..7371
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7440..8039
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7440..7459)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7936..7956
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8177..8410
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8482..8603
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8519..8538)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8573..8592
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8643..8778
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8798..8817)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8799..8817)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8827..8844)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8827..8843)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8836..8858)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8985..9440
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9072..9091)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9282..9303
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9466..9570
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9571..9639,9640..10431)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9789..9808)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10602..11190
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11091..11110
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11344..11361
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1081 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     1861 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     1921 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta
     1981 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     2041 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta
     2101 tcgtccagtg tggtggaatt ctgcagatat caactttgta tagaaaagtt ggcctccgcg
     2161 ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga
     2221 cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag cggcccgctg
     2281 ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag gacgggactt
     2341 gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg aaaagtagtc
     2401 ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat gattatataa
     2461 ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt cgcggttctt
     2521 gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct ggccggggct
     2581 ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc caagggctgt
     2641 agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg cagcaaaatg
     2701 gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga ggtcgttgaa
     2761 acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg
     2821 ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct gacgtgaagt
     2881 ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt tatggcggtg
     2941 ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac
     3001 ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt
     3061 ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc
     3121 cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg gttttatgta
     3181 cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg ttggcgagtg
     3241 tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt
     3301 ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag
     3361 acgcaacttt gtacaaaaaa gcaggctccg aattcgccct tatgaagaag tttttcgact
     3421 cccggcgaga gcagggcggc tctggcctgg gctccggctc cagcggagga gggggcagca
     3481 cctcgggcct gggcagtggc tacatcggaa gagtcttcgg catcgggcga cagcaggtca
     3541 cagtggacga ggtgttggcg gaaggtggat ttgctattgt atttctggtg aggacaagca
     3601 atgggatgaa atgtgccttg aaacgcatgt ttgtcaacaa tgagcatgat ctccaggtgt
     3661 gcaagagaga aatccagata atgagggatc tttcagggca caagaatatt gtgggttaca
     3721 ttgattctag tatcaacaac gtgagtagcg gtgatgtatg ggaagtgctc attctgatgg
     3781 acttttgtag aggtggccag gtggtaaacc tgatgaacca gcgcctgcaa acaggcttta
     3841 cagagaatga agtgctccag atattttgtg atacctgtga agctgttgcc cgcctgcatc
     3901 agtgcaaaac tcctattatc caccgggacc tgaaggttga aaacatcctc ttgcatgacc
     3961 gaggccacta tgtcctgtgt gactttggaa gcgccaccaa caaattccag aatccacaaa
     4021 ctgagggagt caatgcagta gaagatgaga ttaagaaata cacaacgctg tcctatcgag
     4081 caccagaaat ggtcaacctg tacagtggca aaatcatcac tacgaaggca gacatttggg
     4141 ctcttggatg tttgttgtat aaattatgct acttcacttt gccatttggg gaaagtcagg
     4201 tggcaatttg tgatggaaac ttcacaattc ctgataattc tcgatattct caagacatgc
     4261 actgcctaat taggtatatg ttggaaccag accctgacaa aaggccggat atttaccagg
     4321 tgtcctactt ctcatttaag ctactcaaga aagagtgccc aattccaaat gtacagaact
     4381 ctcccattcc tgcaaagctt cctgaaccag tgaaagccag tgaggcagct gcaaaaaaga
     4441 cccagccaaa ggccagactg acagatccca ttcccaccac agagacttca attgcacccc
     4501 gccagaggcc taaagctggg cagactcagc cgaacccagg aatccttccc atccagccag
     4561 cgctgacacc ccggaagagg gccactgttc agcccccacc tcaggctgca ggatccagca
     4621 atcagcctgg ccttttagcc agtgttcccc aaccaaaacc ccaagcccca cccagccagc
     4681 ctctgccgca aactcaggcc aagcagccac aggctcctcc cactccacag cagacgcctt
     4741 ctactcaggc ccagggtctg cccgctcagg cccaggccac accccagcac cagcagcaac
     4801 tcttcctcaa gcagcaacag cagcagcaac agccaccgcc agcacagcag cagccggcag
     4861 gcacgtttta ccagcagcag caggcccaga ctcagcagtt tcaggcagta catccagcaa
     4921 cccagcaacc agcaattgct cagttccctg tggtgtccca aggaggctct caacagcagc
     4981 taatgcagaa tttctaccag cagcagcagc agcagcaaca acaacagcaa cagcaacagc
     5041 tggccacagc cctgcatcaa caacagctga tgactcagca ggctgccttg cagcaaaagc
     5101 ccactatggc agcaggacag cagccccagc cacagccagc tgcagcccca cagccagccc
     5161 ctgcccagga gccagcgatt caagccccag taagacaaca gccaaaggtt cagacaaccc
     5221 cacctcctgc cgtccagggg cagaaagttg gatctctcac tccaccctca tcccccaaaa
     5281 cccaacgtgc tgggcacagg cgtattctca gtgacgtaac ccacagtgca gtctttgggg
     5341 tccctgccag caaatcaacc cagctgctcc aggcagctgc agctgaggcc agtctcaata
     5401 ggtccaagtc tgcaaccact actccatcag gctctcctcg gacctctcaa caaaacgttt
     5461 ataatccttc agaagggtct acgtggaatc cctttgatga cgataatttc tccaaactca
     5521 cagctgaaga actgctaaac aaggactttg ccaagcttgg ggaaggcaaa catcccgaga
     5581 agcttggagg ctcagctgag agtttgatcc caggctttca atcaacccaa ggtgatgctt
     5641 ttgctacgac ctcattttct gctggaactg ctgaaaaaag gaagggtggg cagactgtgg
     5701 actctggcct cccgcttcta agcgtgtctg atcctttcat tcctcttcaa gtacctgatg
     5761 caccagaaaa actaattgag ggactcaagt ctcctgacac ttctcttctg ctccctgacc
     5821 tcttgcctat gacagatcct tttggtagca cttctgatgc tgtaattggt aaagtcatca
     5881 tctctgtttc ttcagtcatg catgatatgt gtgcctgttt caagaatgac aagtacctag
     5941 ttaaccaatc cctggggaat agccctgcca ccccagaagc caaggctatt gaattcaagg
     6001 gcgaattcga cccagctttc ttgtacaaag tgggcaagcc tatccctaac cctctcctcg
     6061 gtctcgattc tacgtaataa cactggatat accaccgttg atatatccca atggcatcgt
     6121 aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca gaccgttcag
     6181 ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt ttatccggcc
     6241 tttattcaca ttcttgcccg cctgtaatca actttattat acatagttga tatccagcac
     6301 agtggcggcc gctcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     6361 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     6421 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     6481 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     6541 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     6601 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     6661 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     6721 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     6781 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     6841 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     6901 ccccgcctgg aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc
     6961 gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat
     7021 tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct
     7081 tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg
     7141 tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa
     7201 tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg
     7261 gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg
     7321 gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct
     7381 gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagcttacca
     7441 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
     7501 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc
     7561 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     7621 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     7681 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     7741 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     7801 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     7861 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     7921 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     7981 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac
     8041 gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat gcatctcgag
     8101 ggcccggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     8161 aaagaaaagg ggggactgga agggctagct cactcccaac gaagacaaga tctgcttttt
     8221 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     8281 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     8341 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     8401 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     8461 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     8521 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     8581 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     8641 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     8701 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     8761 gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc
     8821 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     8881 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     8941 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     9001 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     9061 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     9121 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     9181 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     9241 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     9301 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     9361 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     9421 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     9481 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     9541 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     9601 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     9661 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     9721 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     9781 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     9841 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     9901 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     9961 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
    10021 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
    10081 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
    10141 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
    10201 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
    10261 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
    10321 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
    10381 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
    10441 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
    10501 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
    10561 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
    10621 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
    10681 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
    10741 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
    10801 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
    10861 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
    10921 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
    10981 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
    11041 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
    11101 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
    11161 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
    11221 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
    11281 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
    11341 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
    11401 gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc
    11461 agtgagcgca acgcaat
//
