LOCUS       sequence-267910-       12254 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of AAK1 (human) tagged with C-terminal 
            V5-TurboID for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12254)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 12254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-AAK1-V5-TurboID_IDG-K
FEATURES             Location/Qualifiers
     source          1..12254
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        182..408
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             409..589
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    636..761
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1254..1487
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1672..1716
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1983..2099
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2131..2151
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        2151..3362
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3199..3218
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    3363..3387
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6010..6034)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6036..6077
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6084..7040
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    complement(7046..7066)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    7095..7683
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7148..7168)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7566..7577)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7697..8196
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7732..7755)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8129..8148
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8217..8816
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8217..8236)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8713..8733
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8954..9187
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9259..9380
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9296..9315)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9350..9369
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9420..9555
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9575..9594)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9576..9594)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9604..9621)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9604..9620)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9613..9635)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9762..10217
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9849..9868)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10059..10080
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10243..10347
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10348..10416,10417..11208)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11379..11967
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11868..11887
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12121..12138
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1081 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     1861 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     1921 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta
     1981 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     2041 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta
     2101 tcgtccagtg tggtggaatt ctgcagatat caactttgta tagaaaagtt ggcctccgcg
     2161 ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga
     2221 cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag cggcccgctg
     2281 ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag gacgggactt
     2341 gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg aaaagtagtc
     2401 ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat gattatataa
     2461 ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt cgcggttctt
     2521 gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct ggccggggct
     2581 ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc caagggctgt
     2641 agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg cagcaaaatg
     2701 gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga ggtcgttgaa
     2761 acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg
     2821 ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct gacgtgaagt
     2881 ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt tatggcggtg
     2941 ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac
     3001 ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt
     3061 ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc
     3121 cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg gttttatgta
     3181 cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg ttggcgagtg
     3241 tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt
     3301 ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag
     3361 acgcaacttt gtacaaaaaa gcaggctccg aattcgccct tatgaagaag tttttcgact
     3421 cccggcgaga gcagggcggc tctggcctgg gctccggctc cagcggagga gggggcagca
     3481 cctcgggcct gggcagtggc tacatcggaa gagtcttcgg catcgggcga cagcaggtca
     3541 cagtggacga ggtgttggcg gaaggtggat ttgctattgt atttctggtg aggacaagca
     3601 atgggatgaa atgtgccttg aaacgcatgt ttgtcaacaa tgagcatgat ctccaggtgt
     3661 gcaagagaga aatccagata atgagggatc tttcagggca caagaatatt gtgggttaca
     3721 ttgattctag tatcaacaac gtgagtagcg gtgatgtatg ggaagtgctc attctgatgg
     3781 acttttgtag aggtggccag gtggtaaacc tgatgaacca gcgcctgcaa acaggcttta
     3841 cagagaatga agtgctccag atattttgtg atacctgtga agctgttgcc cgcctgcatc
     3901 agtgcaaaac tcctattatc caccgggacc tgaaggttga aaacatcctc ttgcatgacc
     3961 gaggccacta tgtcctgtgt gactttggaa gcgccaccaa caaattccag aatccacaaa
     4021 ctgagggagt caatgcagta gaagatgaga ttaagaaata cacaacgctg tcctatcgag
     4081 caccagaaat ggtcaacctg tacagtggca aaatcatcac tacgaaggca gacatttggg
     4141 ctcttggatg tttgttgtat aaattatgct acttcacttt gccatttggg gaaagtcagg
     4201 tggcaatttg tgatggaaac ttcacaattc ctgataattc tcgatattct caagacatgc
     4261 actgcctaat taggtatatg ttggaaccag accctgacaa aaggccggat atttaccagg
     4321 tgtcctactt ctcatttaag ctactcaaga aagagtgccc aattccaaat gtacagaact
     4381 ctcccattcc tgcaaagctt cctgaaccag tgaaagccag tgaggcagct gcaaaaaaga
     4441 cccagccaaa ggccagactg acagatccca ttcccaccac agagacttca attgcacccc
     4501 gccagaggcc taaagctggg cagactcagc cgaacccagg aatccttccc atccagccag
     4561 cgctgacacc ccggaagagg gccactgttc agcccccacc tcaggctgca ggatccagca
     4621 atcagcctgg ccttttagcc agtgttcccc aaccaaaacc ccaagcccca cccagccagc
     4681 ctctgccgca aactcaggcc aagcagccac aggctcctcc cactccacag cagacgcctt
     4741 ctactcaggc ccagggtctg cccgctcagg cccaggccac accccagcac cagcagcaac
     4801 tcttcctcaa gcagcaacag cagcagcaac agccaccgcc agcacagcag cagccggcag
     4861 gcacgtttta ccagcagcag caggcccaga ctcagcagtt tcaggcagta catccagcaa
     4921 cccagcaacc agcaattgct cagttccctg tggtgtccca aggaggctct caacagcagc
     4981 taatgcagaa tttctaccag cagcagcagc agcagcaaca acaacagcaa cagcaacagc
     5041 tggccacagc cctgcatcaa caacagctga tgactcagca ggctgccttg cagcaaaagc
     5101 ccactatggc agcaggacag cagccccagc cacagccagc tgcagcccca cagccagccc
     5161 ctgcccagga gccagcgatt caagccccag taagacaaca gccaaaggtt cagacaaccc
     5221 cacctcctgc cgtccagggg cagaaagttg gatctctcac tccaccctca tcccccaaaa
     5281 cccaacgtgc tgggcacagg cgtattctca gtgacgtaac ccacagtgca gtctttgggg
     5341 tccctgccag caaatcaacc cagctgctcc aggcagctgc agctgaggcc agtctcaata
     5401 ggtccaagtc tgcaaccact actccatcag gctctcctcg gacctctcaa caaaacgttt
     5461 ataatccttc agaagggtct acgtggaatc cctttgatga cgataatttc tccaaactca
     5521 cagctgaaga actgctaaac aaggactttg ccaagcttgg ggaaggcaaa catcccgaga
     5581 agcttggagg ctcagctgag agtttgatcc caggctttca atcaacccaa ggtgatgctt
     5641 ttgctacgac ctcattttct gctggaactg ctgaaaaaag gaagggtggg cagactgtgg
     5701 actctggcct cccgcttcta agcgtgtctg atcctttcat tcctcttcaa gtacctgatg
     5761 caccagaaaa actaattgag ggactcaagt ctcctgacac ttctcttctg ctccctgacc
     5821 tcttgcctat gacagatcct tttggtagca cttctgatgc tgtaattggt aaagtcatca
     5881 tctctgtttc ttcagtcatg catgatatgt gtgcctgttt caagaatgac aagtacctag
     5941 ttaaccaatc cctggggaat agccctgcca ccccagaagc caaggctatt gaattcaagg
     6001 gcgaattcga cccagctttc ttgtacaaag tgggcggcaa gcccatcccc aaccccctgc
     6061 tgggcctgga cagcaccgct agcaaagaca atactgtgcc tctgaagctg atcgctctcc
     6121 tggctaatgg cgagttccat agtggcgaac agctgggaga aaccctgggc atgtccaggg
     6181 ccgctatcaa caagcacatt cagactctgc gcgactgggg cgtggacgtg ttcaccgtgc
     6241 ccggaaaggg ctactctctg cccgagccta tcccgctgct gaacgctaaa cagattctgg
     6301 gacagctgga cggcgggagc gtggcagtcc tgcctgtggt cgactccacc aatcagtacc
     6361 tgctggatcg aatcggcgag ctgaagagtg gggatgcttg cattgcagaa tatcagcagg
     6421 cagggagagg aagcagaggg aggaaatggt tctctccttt tggagctaac ctgtacctga
     6481 gtatgttttg gcgcctgaag cggggaccag cagcaatcgg cctgggcccg gtcatcggaa
     6541 ttgtcatggc agaagcgctg cgaaagctgg gagcagacaa ggtgcgagtc aaatggccca
     6601 atgacctgta tctgcaggat agaaagctgg caggcatcct ggtggagctg gccggaataa
     6661 caggcgatgc tgcacagatc gtcattggcg ccgggattaa cgtggctatg aggcgcgtgg
     6721 aggaaagcgt ggtcaatcag ggctggatca cactgcagga agcagggatt aacctggaca
     6781 ggaatactct ggccgctacg ctgatccgag agctgcgggc agccctggaa ctgttcgagc
     6841 aggaaggcct ggctccatat ctgccacggt gggagaagct ggataacttc atcaatagac
     6901 ccgtgaagct gatcattggg gacaaagaga ttttcgggat tagccggggg attgataaac
     6961 agggagccct gctgctggaa caggacggag ttatcaaacc ctggatgggc ggagaaatca
     7021 gtctgcggtc tgccgaaaag taagccaact ttattataca tagttgatat ccagcacagt
     7081 ggcggccgct cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     7141 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     7201 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     7261 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     7321 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     7381 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     7441 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     7501 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     7561 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     7621 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     7681 cgcctggaat tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct
     7741 ttagcagccc cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc
     7801 acatccaccg gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct
     7861 actcctcccc tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga
     7921 caaatggaag tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg
     7981 aagcgggtag gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct
     8041 cagaggctgg gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg
     8101 ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc
     8161 gcgctgttct cctcttcctc atctccgggc ctttcgacct gcagcccaag cttaccatga
     8221 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca
     8281 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc
     8341 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg
     8401 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg
     8461 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc
     8521 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg
     8581 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg
     8641 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga
     8701 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg
     8761 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgacgcc
     8821 cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca tctcgagggc
     8881 ccggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     8941 gaaaaggggg gactggaagg gctagctcac tcccaacgaa gacaagatct gctttttgct
     9001 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     9061 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     9121 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     9181 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     9241 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     9301 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     9361 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     9421 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     9481 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     9541 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg
     9601 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     9661 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     9721 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     9781 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     9841 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     9901 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     9961 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
    10021 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
    10081 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
    10141 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
    10201 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
    10261 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
    10321 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
    10381 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
    10441 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
    10501 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
    10561 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
    10621 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
    10681 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
    10741 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
    10801 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
    10861 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
    10921 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
    10981 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
    11041 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
    11101 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
    11161 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
    11221 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
    11281 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
    11341 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
    11401 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
    11461 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
    11521 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
    11581 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
    11641 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
    11701 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
    11761 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
    11821 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
    11881 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
    11941 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
    12001 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
    12061 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
    12121 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
    12181 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
    12241 gagcgcaacg caat
//
