LOCUS       sequence-267913-       12068 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of AAK1 (human) tagged with C-terminal 
            V5-miniTurbo for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12068)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 12068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p667-UBC-AAK1-V5-miniTurbo_IDG-K
FEATURES             Location/Qualifiers
     source          1..12068
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        182..408
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             409..589
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    636..761
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1254..1487
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1672..1716
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1983..2099
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2131..2151
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        2151..3362
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3199..3218
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    3363..3387
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6010..6034)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6036..6077
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6084..6854
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    complement(6860..6880)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    6909..7497
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6962..6982)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7380..7391)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7511..8010
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7546..7569)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7943..7962
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8031..8630
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8031..8050)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8527..8547
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8768..9001
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9073..9194
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9110..9129)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9164..9183
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9234..9369
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9389..9408)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9390..9408)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9418..9435)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9418..9434)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9427..9449)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9576..10031
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9663..9682)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9873..9894
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10057..10161
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10162..10230,10231..11022)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      11193..11781
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11682..11701
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11935..11952
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1081 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     1861 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     1921 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta
     1981 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     2041 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta
     2101 tcgtccagtg tggtggaatt ctgcagatat caactttgta tagaaaagtt ggcctccgcg
     2161 ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga
     2221 cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag cggcccgctg
     2281 ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag gacgggactt
     2341 gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg aaaagtagtc
     2401 ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat gattatataa
     2461 ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt cgcggttctt
     2521 gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct ggccggggct
     2581 ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc caagggctgt
     2641 agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg cagcaaaatg
     2701 gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga ggtcgttgaa
     2761 acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt cgctaatgcg
     2821 ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct gacgtgaagt
     2881 ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt tatggcggtg
     2941 ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc gtgacgtcac
     3001 ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg cggtaggctt
     3061 ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat cgacaggcgc
     3121 cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg gttttatgta
     3181 cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg ttggcgagtg
     3241 tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca atatgtaatt
     3301 ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct tttttgttag
     3361 acgcaacttt gtacaaaaaa gcaggctccg aattcgccct tatgaagaag tttttcgact
     3421 cccggcgaga gcagggcggc tctggcctgg gctccggctc cagcggagga gggggcagca
     3481 cctcgggcct gggcagtggc tacatcggaa gagtcttcgg catcgggcga cagcaggtca
     3541 cagtggacga ggtgttggcg gaaggtggat ttgctattgt atttctggtg aggacaagca
     3601 atgggatgaa atgtgccttg aaacgcatgt ttgtcaacaa tgagcatgat ctccaggtgt
     3661 gcaagagaga aatccagata atgagggatc tttcagggca caagaatatt gtgggttaca
     3721 ttgattctag tatcaacaac gtgagtagcg gtgatgtatg ggaagtgctc attctgatgg
     3781 acttttgtag aggtggccag gtggtaaacc tgatgaacca gcgcctgcaa acaggcttta
     3841 cagagaatga agtgctccag atattttgtg atacctgtga agctgttgcc cgcctgcatc
     3901 agtgcaaaac tcctattatc caccgggacc tgaaggttga aaacatcctc ttgcatgacc
     3961 gaggccacta tgtcctgtgt gactttggaa gcgccaccaa caaattccag aatccacaaa
     4021 ctgagggagt caatgcagta gaagatgaga ttaagaaata cacaacgctg tcctatcgag
     4081 caccagaaat ggtcaacctg tacagtggca aaatcatcac tacgaaggca gacatttggg
     4141 ctcttggatg tttgttgtat aaattatgct acttcacttt gccatttggg gaaagtcagg
     4201 tggcaatttg tgatggaaac ttcacaattc ctgataattc tcgatattct caagacatgc
     4261 actgcctaat taggtatatg ttggaaccag accctgacaa aaggccggat atttaccagg
     4321 tgtcctactt ctcatttaag ctactcaaga aagagtgccc aattccaaat gtacagaact
     4381 ctcccattcc tgcaaagctt cctgaaccag tgaaagccag tgaggcagct gcaaaaaaga
     4441 cccagccaaa ggccagactg acagatccca ttcccaccac agagacttca attgcacccc
     4501 gccagaggcc taaagctggg cagactcagc cgaacccagg aatccttccc atccagccag
     4561 cgctgacacc ccggaagagg gccactgttc agcccccacc tcaggctgca ggatccagca
     4621 atcagcctgg ccttttagcc agtgttcccc aaccaaaacc ccaagcccca cccagccagc
     4681 ctctgccgca aactcaggcc aagcagccac aggctcctcc cactccacag cagacgcctt
     4741 ctactcaggc ccagggtctg cccgctcagg cccaggccac accccagcac cagcagcaac
     4801 tcttcctcaa gcagcaacag cagcagcaac agccaccgcc agcacagcag cagccggcag
     4861 gcacgtttta ccagcagcag caggcccaga ctcagcagtt tcaggcagta catccagcaa
     4921 cccagcaacc agcaattgct cagttccctg tggtgtccca aggaggctct caacagcagc
     4981 taatgcagaa tttctaccag cagcagcagc agcagcaaca acaacagcaa cagcaacagc
     5041 tggccacagc cctgcatcaa caacagctga tgactcagca ggctgccttg cagcaaaagc
     5101 ccactatggc agcaggacag cagccccagc cacagccagc tgcagcccca cagccagccc
     5161 ctgcccagga gccagcgatt caagccccag taagacaaca gccaaaggtt cagacaaccc
     5221 cacctcctgc cgtccagggg cagaaagttg gatctctcac tccaccctca tcccccaaaa
     5281 cccaacgtgc tgggcacagg cgtattctca gtgacgtaac ccacagtgca gtctttgggg
     5341 tccctgccag caaatcaacc cagctgctcc aggcagctgc agctgaggcc agtctcaata
     5401 ggtccaagtc tgcaaccact actccatcag gctctcctcg gacctctcaa caaaacgttt
     5461 ataatccttc agaagggtct acgtggaatc cctttgatga cgataatttc tccaaactca
     5521 cagctgaaga actgctaaac aaggactttg ccaagcttgg ggaaggcaaa catcccgaga
     5581 agcttggagg ctcagctgag agtttgatcc caggctttca atcaacccaa ggtgatgctt
     5641 ttgctacgac ctcattttct gctggaactg ctgaaaaaag gaagggtggg cagactgtgg
     5701 actctggcct cccgcttcta agcgtgtctg atcctttcat tcctcttcaa gtacctgatg
     5761 caccagaaaa actaattgag ggactcaagt ctcctgacac ttctcttctg ctccctgacc
     5821 tcttgcctat gacagatcct tttggtagca cttctgatgc tgtaattggt aaagtcatca
     5881 tctctgtttc ttcagtcatg catgatatgt gtgcctgttt caagaatgac aagtacctag
     5941 ttaaccaatc cctggggaat agccctgcca ccccagaagc caaggctatt gaattcaagg
     6001 gcgaattcga cccagctttc ttgtacaaag tgggcggcaa gcccatcccc aaccccctgc
     6061 tgggcctgga cagcaccgct agcatcccgc tgctgaacgc taaacagatt ctgggacagc
     6121 tggacggcgg gagcgtggca gtcctgcctg tggtcgactc caccaatcag tacctgctgg
     6181 atcgaatcgg cgagctgaag agtggggatg cttgcattgc agaatatcag caggcaggga
     6241 gaggaagcag agggaggaaa tggttctctc cttttggagc taacctgtac ctgagtatgt
     6301 tttggcgcct gaagcgggga ccagcagcaa tcggcctggg cccggtcatc ggaattgtca
     6361 tggcagaagc gctgcgaaag ctgggagcag acaaggtgcg agtcaaatgg cccaatgacc
     6421 tgtatctgca ggatagaaag ctggcaggca tcctggtgga gctggccgga ataacaggcg
     6481 atgctgcaca gatcgtcatt ggcgccggga ttaacgtggc tatgaggcgc gtggaggaaa
     6541 gcgtggtcaa tcagggctgg atcacactgc aggaagcagg gattaacctg gacaggaata
     6601 ctctggccgc tatgctgatc cgagagctgc gggcagccct ggaactgttc gagcaggaag
     6661 gcctggctcc atatctgtca cggtgggaga agctggataa cttcatcaat agacccgtga
     6721 agctgatcat tggggacaaa gagattttcg ggattagccg ggggattgat aaacagggag
     6781 ccctgctgct ggaacaggac ggagttatca aaccctggat gggcggagaa atcagtctgc
     6841 ggtctgccga aaagtaagcc aactttatta tacatagttg atatccagca cagtggcggc
     6901 cgctcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     6961 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     7021 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     7081 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     7141 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     7201 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     7261 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     7321 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     7381 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     7441 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     7501 gaattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca
     7561 gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc
     7621 accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct
     7681 cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg
     7741 gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg
     7801 gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg
     7861 ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc
     7921 cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg
     7981 ttctcctctt cctcatctcc gggcctttcg acctgcagcc caagcttacc atgaccgagt
     8041 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg
     8101 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg
     8161 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg
     8221 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag
     8281 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg
     8341 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt
     8401 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg
     8461 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg
     8521 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg
     8581 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga cgcccgcccc
     8641 acgacccgca gcgcccgacc gaaaggagcg cacgacccca tgcatctcga gggcccggta
     8701 cctttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     8761 gggggactgg aagggctagc tcactcccaa cgaagacaag atctgctttt tgcttgtact
     8821 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     8881 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     8941 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     9001 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     9061 gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     9121 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     9181 atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc
     9241 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     9301 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt
     9361 ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact
     9421 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     9481 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     9541 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
     9601 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     9661 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     9721 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     9781 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     9841 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     9901 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     9961 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
    10021 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
    10081 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
    10141 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
    10201 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
    10261 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
    10321 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
    10381 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
    10441 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
    10501 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
    10561 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
    10621 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
    10681 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
    10741 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
    10801 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
    10861 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
    10921 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
    10981 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
    11041 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
    11101 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
    11161 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
    11221 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
    11281 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
    11341 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
    11401 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
    11461 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
    11521 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
    11581 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
    11641 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
    11701 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
    11761 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
    11821 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
    11881 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
    11941 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
    12001 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
    12061 aacgcaat
//
