LOCUS       sequence-267926-       11996 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible ZF-m6A Writer. Use abscisic acid (ABA) to direct GATC 
            methylation to ZF binding sites. 
            pMinCMV-NLS-ABI1cs-ZF-NLS-P2A-Dam(N132A)-PYL1cs-HA pGK-BlastR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11996)
  AUTHORS   Park M, Patel N, Keung AJ, Khalil AS
  TITLE     Engineering Epigenetic Regulation Using Synthetic Read-Write 
            Modules.
  JOURNAL   Cell. 2019 Jan 10;176(1-2):227-238.e20. doi: 
            10.1016/j.cell.2018.11.002. Epub 2018 Dec 6.
  PUBMED    30528434
REFERENCE   2  (bases 1 to 11996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2019-01-10"; volume: "176(1-2):227-238.e20. doi"; pages: " 
            10.1016/j.cell.2018.11.002. Epub 2018 Dec 6"
COMMENT     Sequence Label: pMP597
FEATURES             Location/Qualifiers
     source          1..11996
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(144..163)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        335..714
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        716..914
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     869..889
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             932..1112
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1159..1284
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1777..2010
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2195..2239
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2537..2654
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     2732..2756
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2796..2816
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4266..4286
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4299..4355
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5751..5777
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     promoter        5836..6335
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5871..5894)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6268..6287
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6362..6760
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    6785..7373
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6838..6858)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7256..7267)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7376..7392)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7377..7393)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7898..8078
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8104..8121)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8110..8334
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8380..8808
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8467..8486)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8677..8698
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8817..8837)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8822..9151
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9002..9137
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9064..9083
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9199..9246
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9265..9639
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9769..9890
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9806..9825)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9860..9879
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9939..9955)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9939..9955)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9952..9974)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9963..9979
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9987..9993,9994..10011,10012..10017))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10032..10053
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10170..10187)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10341..10929)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10421..10440)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11100..11891,11892..11960))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11723..11742
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(11961..11996,1..69))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
       61 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc
      121 gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
      181 ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca
      241 acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg
      301 ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa
      361 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
      421 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
      481 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
      541 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc
      601 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
      661 tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
      721 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
      781 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
      841 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
      901 gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc agatctgagc
      961 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     1021 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     1081 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     1141 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     1201 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     1261 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     1321 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     1381 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     1441 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     1501 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     1561 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     1621 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     1681 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     1741 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     1801 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     1861 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     1921 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     1981 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     2041 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     2101 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     2161 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     2221 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     2281 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     2341 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     2401 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     2461 cagatccatt cgattagtga acggatcggc actgcgtgcg ccaattctgc agacaaatgg
     2521 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     2581 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     2641 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccagtttgg ttaattaata
     2701 ggcgtgtacg gtgggaggcc tatataagca gagctcgttt agtgaaccgt cagatcgcct
     2761 ggaacgcgtt taattaagcc gccaccatgt tcgaacccaa gaagaagaga aaggtgttcg
     2821 aaactagtgt gcccctgtat ggcttcactt ccatttgtgg ccgacggcct gaaatggaag
     2881 ccgcggtgtc aaccatacca cggtttctgc agagctcatc aggctccatg ctggacggac
     2941 gctttgatcc acagtctgcc gcacatttct ttggagtcta cgacggccac gggggcagcc
     3001 aggtcgccaa ctactgcagg gaaaggatgc atttggcact tgccgaagag atcgccaaag
     3061 agaagcccat gttgtgtgat ggggatacct ggctggagaa gtggaagaaa gcgcttttta
     3121 actcttttct gagagtggat tctgagatag aatctgtcgc acccgagacc gtgggcagca
     3181 catccgtcgt agccgtagtg tttccctccc acatattcgt cgccaactgc ggcgacagtc
     3241 gagccgtcct ctgccgaggt aagaccgccc tgcctctgag tgttgaccat aagcccgacc
     3301 gggaggatga ggccgcccga atcgaggccg ccggtggaaa agtcatccaa tggaacggcg
     3361 caagagtgtt cggcgtgctg gcgatgtcca ggagcattgg agaccggtac ctgaagccca
     3421 gcataatccc agatcccgaa gtgaccgcag tcaagagggt gaaagaggac gattgtctga
     3481 tcctggctag cgatggcgta tgggacgtga tgactgatga ggaggcgtgt gaaatggccc
     3541 gcaagcgaat cctgctgtgg cataaaaaaa acgcagtcgc gggggacgct tctcttctgg
     3601 cagacgaaag gcgcaaagaa ggtaaagacc cggctgctat gagcgccgcc gaatatctca
     3661 gtaagctggc aattcagcga gggtccaaag acaacatttc cgtggtcgtg gtagacctca
     3721 aaggcggttc cggcggttct agacccggag agcgcccatt ccagtgtcgg atttgcatgc
     3781 ggaacttttc gagaagacac ggcctggaca gacatacccg tactcataca ggtgaaaaac
     3841 cctttcagtg tcggatctgt atgcgaaatt tctccgacca cagcagcctg aagagacatc
     3901 tacgtaccca caccggcagc cagaagccat ttcagtgtcg gatctgtatg cggaacttct
     3961 ccgtgagaca caacctgacc agacatctac gtacgcacac cggagagaag ccattccaat
     4021 gccgaatatg catgcgcaac ttcagtgacc acagcaacct gagcagacac ctaaaaaccc
     4081 acaccggttc ccagaagcca tttcagtgtc ggatctgtat gcggaacttc tcccagcgca
     4141 gcagcctggt gagacatcta cgtacgcaca ccggagagaa gccattccaa tgccgaatat
     4201 gcatgcgcaa cttcagtgag agcggccacc tgaagagaca cctgcgtacg cacctgaggg
     4261 gatccccgaa gaaaaaacgg aaagtgggcg gaagcggagc tactaacttc agcctgctga
     4321 agcaggctgg agacgtggag gagaaccctg gacctggtca ccataagaaa aatcgcgctt
     4381 ttttgaagtg ggcagggggc aagtatcccc tgcttgatga tattaaacgg catttgccca
     4441 agggcgaatg tctggttgag ccttttgtag gtgccgggtc ggtgtttctc aacaccgact
     4501 tttctcgtta tatccttgcc gatatcaata gcgacctgat cagtctctat aacattgtga
     4561 agatgcgtac tgatgagtac gtacaggccg cacgcgagct gtttgttccc gaaacaaatt
     4621 gcgccgaggt ttactatcag ttccgcgaag agttcaacaa aagccaggat ccgttccgtc
     4681 gggcggtact gtttttatat ttgaaccgct acggttacaa cggcctgtgt cgttacaatc
     4741 tgcgcggtga gtttgccgtg ccgttcggcc gctacaaaaa accctatttc ccggaagcag
     4801 agttgtatca cttcgctgaa aaagcgcaga atgccttttt ctattgtgag tcttacgccg
     4861 atagcatggc gcgcgcagat gatgcatccg tcgtctattg cgatccgcct tatgcaccgc
     4921 tgtctgcgac cgccaacttt acggcgtatc acacaaacag ttttacgctt gaacaacaag
     4981 cgcatctggc ggagatcgcc gaaggtctgg ttgagcgcca tattccagtg ctgatctcca
     5041 atcacgatac gatgttaacg cgtgagtggt atcagcgcgc aaaattgcat gtcgtcaaag
     5101 ttcgacgcag tataagcagc aacggcggca cacgtaaaaa ggtggacgaa ctgctggctt
     5161 tgtacaaacc aggagtcgtt tcacccgcga aaaaaggtgg tggcagcggt caattgactc
     5221 aagacgaatt cacccaactc tcccaatcaa tcgccgagtt ccacacgtac caactcggta
     5281 acggccgttg ctcatctctc ctagctcagc gaatccacgc gccgccggaa acagtatggt
     5341 ccgtggtgag acgtttcgat aggccacaga tttacaaaca cttcatcaaa agctgtaacg
     5401 tgagtgaaga tttcgagatg cgagtgggat gcacgcgcga cgtgaacgtg ataagtggat
     5461 taccggcgaa tacgtctcga gagagattag atctgttgga cgatgatcgg agagtgactg
     5521 ggtttagtat aaccggtggt gaacataggc tgaggaatta taaatcggtt acgacggttc
     5581 atagatttga gaaagaagaa gaagaagaaa ggatctggac cgttgttttg gaatcttatg
     5641 ttgttgatgt accggaaggt aattcggagg aagatacgag attgtttgct gatacggtta
     5701 ttagattgaa tcttcagaaa cttgcttcga tcactgaagc tatgaacggt tatccgtacg
     5761 acgtaccaga ctacgcataa ggctcgaagg gtgcctgcag ggccggctct agatcgcgaa
     5821 cgcgtgaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt
     5881 tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca
     5941 catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta
     6001 ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac
     6061 aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga
     6121 agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc
     6181 agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg
     6241 gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg
     6301 cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagcccaagc ttcctaggac
     6361 catggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat
     6421 caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct ctagcgacgg
     6481 ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt
     6541 ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg
     6601 aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct
     6661 gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat
     6721 tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa gaattcgata tcaagcttat
     6781 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     6841 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     6901 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     6961 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     7021 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     7081 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     7141 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     7201 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     7261 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     7321 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
     7381 ccgtcgacct cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac
     7441 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac
     7501 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     7561 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga
     7621 tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc
     7681 agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga
     7741 gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca
     7801 tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt
     7861 tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat
     7921 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     7981 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     8041 cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa acccgctgat
     8101 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     8161 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     8221 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     8281 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     8341 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     8401 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     8461 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     8521 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     8581 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     8641 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     8701 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     8761 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
     8821 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     8881 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     8941 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     9001 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     9061 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     9121 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
     9181 cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa
     9241 tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc
     9301 gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac
     9361 ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg
     9421 gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac
     9481 gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg
     9541 gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc
     9601 ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat
     9661 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     9721 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     9781 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     9841 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     9901 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     9961 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
    10021 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
    10081 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
    10141 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
    10201 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
    10261 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
    10321 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
    10381 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
    10441 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
    10501 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
    10561 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
    10621 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
    10681 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
    10741 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
    10801 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
    10861 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
    10921 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    10981 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
    11041 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
    11101 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
    11161 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
    11221 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
    11281 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
    11341 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
    11401 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
    11461 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
    11521 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    11581 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    11641 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    11701 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    11761 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    11821 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    11881 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    11941 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatca
//
