LOCUS       sequence-267931-        9998 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dCas9-m6A Writer. Methylates adenines at local GATC sites. 
            pCAG-dCas9-NLS-3xFLAG-3AC3L linker-EcoDam(N132A).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9998)
  AUTHORS   Park M, Patel N, Keung AJ, Khalil AS
  TITLE     Engineering Epigenetic Regulation Using Synthetic Read-Write 
            Modules.
  JOURNAL   Cell. 2019 Jan 10;176(1-2):227-238.e20. doi: 
            10.1016/j.cell.2018.11.002. Epub 2018 Dec 6.
  PUBMED    30528434
REFERENCE   2  (bases 1 to 9998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2019-01-10"; volume: "176(1-2):227-238.e20. doi"; pages: " 
            10.1016/j.cell.2018.11.002. Epub 2018 Dec 6"
COMMENT     Sequence Label: pMP416
FEATURES             Location/Qualifiers
     source          1..9998
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        385..658
                     /label=chicken beta-actin promoter
     intron          659..1675
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1599..1621
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1683..1702
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1768..1777
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1774..5175,5176..5178,5179..5775,5776,5777..5779,
                     5780..5781,5782..5784,5785..5877)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1774..1800,1801..1803,1804..4290,4291..4293,
                     4294..5877)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             5884..5904
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5911..5976
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(7019..7038)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    7084..7139
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7138..7157)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7500..7516)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7500..7516)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7513..7535)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7524..7540
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7548..7554,7555..7572,7573..7578))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7593..7614
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7673..7868
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7719..7854
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7874..8008
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7911..7930)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7965..7984
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8076..8093)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8247..8835)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8327..8346)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9006..9797,9798..9866))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9629..9648
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9867..9971)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
      421 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
      481 cgatgggggc gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg
      541 cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc
      601 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg
      661 agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag
      841 ccttgagggg ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt
      901 gtgtgtgtgc gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg
     1021 cggtgccccg cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg
     1081 cgtggggggg tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc
     1141 ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg
     1201 cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg
     1261 gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggcccccgga gcgccggcgg
     1321 ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg agagggcgca
     1381 gggacttcct ttgtcccaaa tctgtgcgga gccgaaatct gggaggcgcc gccgcacccc
     1441 ctctagcggg cgcggggcga agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg
     1501 ccttcgtgcg tcgccgcgcc gccgtcccct tctccctctc cagcctcggg gctgtccgcg
     1561 gggggacggc tgccttcggg ggggacgggg cagggcgggg ttcggcttct ggcgtgtgac
     1621 cggcggctct agagcctctg ctaaccatgt tcatgccttc ttctttttcc tacagctcct
     1681 gggcaacgtg ctggttattg tgctgtctca tcattttggc aaagaattct gcagtcgacg
     1741 gtaccgcggg cccgggatcc accggtcgcc accatggata aaaagtattc tattggttta
     1801 gccatcggca ctaattccgt tggatgggct gtcataaccg atgaatacaa agtaccttca
     1861 aagaaattta aggtgttggg gaacacagac cgtcattcga ttaaaaagaa tcttatcggt
     1921 gccctcctat tcgatagtgg cgaaacggca gaggcgactc gcctgaaacg aaccgctcgg
     1981 agaaggtata cacgtcgcaa gaaccgaata tgttacttac aagaaatttt tagcaatgag
     2041 atggccaaag ttgacgattc tttctttcac cgtttggaag agtccttcct tgtcgaagag
     2101 gacaagaaac atgaacggca ccccatcttt ggaaacatag tagatgaggt ggcatatcat
     2161 gaaaagtacc caacgattta tcacctcaga aaaaagctag ttgactcaac tgataaagcg
     2221 gacctgaggt taatctactt ggctcttgcc catatgataa agttccgtgg gcactttctc
     2281 attgagggtg atctaaatcc ggacaactcg gatgtcgaca aactgttcat ccagttagta
     2341 caaacctata atcagttgtt tgaagagaac cctataaatg caagtggcgt ggatgcgaag
     2401 gctattctta gcgcccgcct ctctaaatcc cgacggctag aaaacctgat cgcacaatta
     2461 cccggagaga agaaaaatgg gttgttcggt aaccttatag cgctctcact aggcctgaca
     2521 ccaaatttta agtcgaactt cgacttagct gaagatgcca aattgcagct tagtaaggac
     2581 acgtacgatg acgatctcga caatctactg gcacaaattg gagatcagta tgcggactta
     2641 tttttggctg ccaaaaacct tagcgatgca atcctcctat ctgacatact gagagttaat
     2701 actgagatta ccaaggcgcc gttatccgct tcaatgatca aaaggtacga tgaacatcac
     2761 caagacttga cacttctcaa ggccctagtc cgtcagcaac tgcctgagaa atataaggaa
     2821 atattctttg atcagtcgaa aaacgggtac gcaggttata ttgacggcgg agcgagtcaa
     2881 gaggaattct acaagtttat caaacccata ttagagaaga tggatgggac ggaagagttg
     2941 cttgtaaaac tcaatcgcga agatctactg cgaaagcagc ggactttcga caacggtagc
     3001 attccacatc aaatccactt aggcgaattg catgctatac ttagaaggca ggaggatttt
     3061 tatccgttcc tcaaagacaa tcgtgaaaag attgagaaaa tcctaacctt tcgcatacct
     3121 tactatgtgg gacccctggc ccgagggaac tctcggttcg catggatgac aagaaagtcc
     3181 gaagaaacga ttactccatg gaattttgag gaagttgtcg ataaaggtgc gtcagctcaa
     3241 tcgttcatcg agaggatgac caactttgac aagaatttac cgaacgaaaa agtattgcct
     3301 aagcacagtt tactttacga gtatttcaca gtgtacaatg aactcacgaa agttaagtat
     3361 gtcactgagg gcatgcgtaa acccgccttt ctaagcggag aacagaagaa agcaatagta
     3421 gatctgttat tcaagaccaa ccgcaaagtg acagttaagc aattgaaaga ggactacttt
     3481 aagaaaattg aatgcttcga ttctgtcgag atctccgggg tagaagatcg atttaatgcg
     3541 tcacttggta cgtatcatga cctcctaaag ataattaaag ataaggactt cctggataac
     3601 gaagagaatg aagatatctt agaagatata gtgttgactc ttaccctctt tgaagatcgg
     3661 gaaatgattg aggaaagact aaaaacatac gctcacctgt tcgacgataa ggttatgaaa
     3721 cagttaaaga ggcgtcgcta tacgggctgg ggacgattgt cgcggaaact tatcaacggg
     3781 ataagagaca agcaaagtgg taaaactatt ctcgattttc taaagagcga cggcttcgcc
     3841 aataggaact ttatgcagct gatccatgat gactctttaa ccttcaaaga ggatatacaa
     3901 aaggcacagg tttccggaca aggggactca ttgcacgaac atattgcgaa tcttgctggt
     3961 tcgccagcca tcaaaaaggg catactccag acagtcaaag tagtggatga gctagttaag
     4021 gtcatgggac gtcacaaacc ggaaaacatt gtaatcgaga tggcacgcga aaatcaaacg
     4081 actcagaagg ggcaaaaaaa cagtcgagag cggatgaaga gaatagaaga gggtattaaa
     4141 gaactgggca gccagatctt aaaggagcat cctgtggaaa atacccaatt gcagaacgag
     4201 aaactttacc tctattacct acaaaatgga agggacatgt atgttgatca ggaactggac
     4261 ataaaccgtt tatctgatta cgacgtcgat gccattgtac cccaatcctt tttgaaggac
     4321 gattcaatcg acaataaagt gcttacacgc tcggataaga accgagggaa aagtgacaat
     4381 gttccaagcg aggaagtcgt aaagaaaatg aagaactatt ggcggcagct cctaaatgcg
     4441 aaactgataa cgcaaagaaa gttcgataac ttaactaaag ctgagagggg tggcttgtct
     4501 gaacttgaca aggccggatt tattaaacgt cagctcgtgg aaacccgcca aatcacaaag
     4561 catgttgcac agatactaga ttcccgaatg aatacgaaat acgacgagaa cgataagctg
     4621 attcgggaag tcaaagtaat cactttaaag tcaaaattgg tgtcggactt cagaaaggat
     4681 tttcaattct ataaagttag ggagataaat aactaccacc atgcgcacga cgcttatctt
     4741 aatgccgtcg tagggaccgc actcattaag aaatacccga agctagaaag tgagtttgtg
     4801 tatggtgatt acaaagttta tgacgtccgt aagatgatcg cgaaaagcga acaggagata
     4861 ggcaaggcta cagccaaata cttcttttat tctaacatta tgaatttctt taagacggaa
     4921 atcactctgg caaacggaga gatacgcaaa cgacctttaa ttgaaaccaa tggggagaca
     4981 ggtgaaatcg tatgggataa gggccgggac ttcgcgacgg tgagaaaagt tttgtccatg
     5041 ccccaagtca acatagtaaa gaaaactgag gtgcagaccg gagggttttc aaaggaatcg
     5101 attcttccaa aaaggaatag tgataagctc atcgctcgta aaaaggactg ggacccgaaa
     5161 aagtacggtg gcttcgatag ccctacagtt gcctattctg tcctagtagt ggcaaaagtt
     5221 gagaagggaa aatccaagaa actgaagtca gtcaaagaat tattggggat aacgattatg
     5281 gagcgctcgt cttttgaaaa gaaccccatc gacttccttg aggcgaaagg ttacaaggaa
     5341 gtaaaaaagg atctcataat taaactacca aagtatagtc tgtttgagtt agaaaatggc
     5401 cgaaaacgga tgttggctag cgccggagag cttcaaaagg ggaacgaact cgcactaccg
     5461 tctaaatacg tgaatttcct gtatttagcg tcccattacg agaagttgaa aggttcacct
     5521 gaagataacg aacagaagca actttttgtt gagcagcaca aacattatct cgacgaaatc
     5581 atagagcaaa tttcggaatt cagtaagaga gtcatcctag ctgatgccaa tctggacaaa
     5641 gtattaagcg catacaacaa gcacagggat aaacccatac gtgagcaggc ggaaaatatt
     5701 atccatttgt ttactcttac caacctcggc gctccagccg cattcaagta ttttgacaca
     5761 acgatagatc gcaaacgata cacttctacc aaggaggtgc tagacgcgac actgattcac
     5821 caatccatca cgggattata tgaaactcgg atagatttgt cacagcttgg gggtgacgga
     5881 tcccccaaga agaagaggaa agtctcgagc gactacaaag accatgacgg tgattataaa
     5941 gatcatgaca tcgattacaa ggatgacgat gacaaggctg caggaggcgg tggaagcggg
     6001 ggagagctct ctagtggcaa tagcaacgcc aactcccggg gccccagttt ttcctccgga
     6061 ctggtgcccc tgtccctgag agggtcacat gcctgcagga agaaaaatcg cgcttttttg
     6121 aagtgggcag ggggcaagta tcccctgctt gatgatatta aacggcattt gcccaagggc
     6181 gaatgtctgg ttgagccttt tgtaggtgcc gggtcggtgt ttctcaacac cgacttttct
     6241 cgttatatcc ttgccgatat caatagcgac ctgatcagtc tctataacat tgtgaagatg
     6301 cgtactgatg agtacgtaca ggccgcacgc gagctgtttg ttcccgaaac aaattgcgcc
     6361 gaggtttact atcagttccg cgaagagttc aacaaaagcc aggatccgtt ccgtcgggcg
     6421 gtactgtttt tatatttgaa ccgctacggt tacaacggcc tgtgtcgtta caatctgcgc
     6481 ggtgagtttg ccgtgccgtt cggccgctac aaaaaaccct atttcccgga agcagagttg
     6541 tatcacttcg ctgaaaaagc gcagaatgcc tttttctatt gtgagtctta cgccgatagc
     6601 atggcgcgcg cagatgatgc atccgtcgtc tattgcgatc cgccttatgc accgctgtct
     6661 gcgaccgcca actttacggc gtatcacaca aacagtttta cgcttgaaca acaagcgcat
     6721 ctggcggaga tcgccgaagg tctggttgag cgccatattc cagtgctgat ctccaatcac
     6781 gatacgatgt taacgcgtga gtggtatcag cgcgcaaaat tgcatgtcgt caaagttcga
     6841 cgcagtataa gcagcaacgg cggcacacgt aaaaaggtgg acgaactgct ggctttgtac
     6901 aaaccaggag tcgtttcacc cgcgaaaaaa taagcggccg cactcctcag gtgcaggctg
     6961 cctatcagaa ggtggtggct ggtgtggcca atgccctggc tcacaaatac cactgagatc
     7021 tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg
     7081 gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac
     7141 tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga
     7201 gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc
     7261 atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt
     7321 gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat
     7381 tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag
     7441 ctgtccctct tctcttatgg agatccctcg acctgcagcc caagcttggc gtaatcatgg
     7501 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     7561 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     7621 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagcggat ccgcatctca
     7681 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     7741 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     7801 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     7861 tttgcaaaaa gctaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     7921 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     7981 catcaatgta tcttatcatg tctggatccg ctgcattaat gaatcggcca acgcgcgggg
     8041 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     8101 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     8161 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     8221 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8281 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8341 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8401 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8461 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8521 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8581 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8641 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     8701 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8761 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8821 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     8881 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     8941 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     9001 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     9061 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     9121 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     9181 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     9241 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     9301 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9361 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9421 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9481 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9541 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9601 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     9661 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9721 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9781 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     9841 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     9901 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     9961 ggggttccgc gcacatttcc ccgaaaagtg ccacctgg
//
