LOCUS       sequence-267943-        9613 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expressing cysJ-infA functional variant 5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9613)
  AUTHORS   Blazejewski T, Ho HI, Wang HH
  TITLE     Synthetic sequence entanglement augments stability and containment 
            of genetic information in cells.
  JOURNAL   Science. 2019 Aug 9;365(6453):595-598. doi: 10.1126/science.aav5477.
  PUBMED    31395784
REFERENCE   2  (bases 1 to 9613)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2019-08-9- 9"; volume: "365"; issue: 
            "6453"; pages: "595-598"
COMMENT     Sequence Label: CI-5
FEATURES             Location/Qualifiers
     source          1..9613
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..56
                     /gene="tetR"
                     /label=tetR/tetA promoters
                     /note="overlapping promoters for bacterial tetR and tetA"
     protein_bind    37..55
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     RBS             74..85
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      1918..1989
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2005..2032
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      2069..2155
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(2693..3643)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILHYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      3691..3913
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(4415..4449)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     terminator      complement(4488..4582)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(4603..4977)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(4978..5082)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(5119..5997)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        6024..6308
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     6231..6250
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             6335..6343
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      6594..6665
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      6681..6708
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     primer_bind     complement(6736..6753)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6870..7415)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(6950..6969)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(7529..7623)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      complement(7688..7782)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(7813..8604)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVITEVSTQLSEVVGAIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             complement(8972..9598)
                     /codon_start=1
                     /gene="tetR"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MMSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGT
                     RPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPT
                     TDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     9503..9522
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 gttgacactc tatcgttgat agagttattt taccactccc tatcagtgat agagaaaaga
       61 attcaaaaga tctaaagagg agaaaggatc tatgacgaca caggtcccac cttccgcgtt
      121 gcttccgttg aacccggagc aactggcacg ccttcaggcg gccacgaccg atttaactcc
      181 cacccagctt gcctgggttt ctggctattt ctggggcgta ctcaatcagc agcctgctgc
      241 gcttgcagcg acgccagcgc cagccgcaga aatgccgggt ataactatta tctccgcctc
      301 gcaaaccggc aatgcgcgcc gggttgctga agcattacgt gatgatttat tagcagcaaa
      361 actgaacgtt aagctggtga acgcgggcga ctataaattc aaacaaatcg ccagcgaaaa
      421 actgctcatc gtagtgacgt caacgcaagg ggaaggggaa ccgccggaag aagccgtcgc
      481 gctgcataag ttcctgttct ccaaaaaagc gccaaagctg gaaaacaccg cgtttgccgt
      541 gtttagcctc ggcgatagct cttatgaatt tttctgccag tccgggaaag atttcgacag
      601 caagctggcg gaactgggtg gtgaacgcct gctcgaccgt gtcgatgccg acgtagaata
      661 ccagaaagga ggatcagaat ggcgaaagaa gatagtagaa gtgctcaagg caagagtacc
      721 agtagcctcc ccagcgcaag ctgccgcgtc cgctgcagtg aacagcacga tctcaacgtc
      781 accatacaca aaagaagctc ccttaacagc atctctagca gtgaatcaaa aaataacagg
      841 tcgggactca ctcaaagacg tccgtcacat agaaatatcg tatttagata gcgggatgcg
      901 ttaccagccg ggtgacgcgc tgggcgtctg gtatcagaac gatccggcac tggtgaaaga
      961 acttgtcgaa ctgctgtggc tgaaaggcga tgaacctgtc accgtcgagg gcaaaacgtt
     1021 gcctctgaac gaagcgctac agtggcactt cgaactgacc gtcaacaccg ccaacattgt
     1081 tgagaattac gccacgctta cccgcagtga aacactgctg ccgctggtgg gcgataaagc
     1141 gaagttacag cattacgccg cgacgacgcc gattgtcgac atggtgcgtt tctccccggc
     1201 acagcttgat gccgaagcgc taattaatct gctgcgcccg ctgacgccgc gtctgtattc
     1261 catcgcctcc tcgcaggcgg aagtcgagaa cgaagtacac gtcaccgttg gtgtggtgcg
     1321 ttacgacgtg gaaggccgcg cccgtgccgg tggtgcctcc agcttcctcg ctgaccgcgt
     1381 ggaagaagag ggcgaagtcc gcgtatttat cgaacataac gataacttcc gcctgccagc
     1441 caatccagaa accccggtga ttatgattgg cccaggcacc ggtattgcgc cgttccgcgc
     1501 ctttatgcag caacgcgccg ccgacgaagc gccaggtaaa aactggctgt tctttggtaa
     1561 tccgcacttt acggaagact tcctgtacca ggtggagtgg cagcgctacg tcaaagatgg
     1621 cgtgctgaca cgtatcgatc ttgcctggtc gcgcgatcaa aaagaaaaag tttacgtaca
     1681 agacaaactg cgcgaacagg gcgcggagct gtggcgctgg atcaatgatg gtgcccacat
     1741 ttatgtctgc ggcgacgcta atcgcatggc gaaagacgtt gagcaggcac ttctggaagt
     1801 gattgccgaa tttggtggca tggacaccga agcggcggat gaatttttaa gtgagctgcg
     1861 cgtagagcgc cgttatcagc gagatgtcta ctaactcgag taaggatctc caggcatcaa
     1921 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     1981 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatacc
     2041 tatcccatgg tacgcgtgct agaggcatca aataaaacga aaggctcagt cgaaagactg
     2101 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc
     2161 gccctagacc tagggtacgg gttttgctgc ccgcaaactg gctgttctgg tgttgctagt
     2221 ttgttatcag aatcgcagat ccggcttcag ccggtttgcc ggctgaaagc gctatttctt
     2281 ccagaattgc catgattttt tccccacggg aggcgtcact ggctcccgtg ttgtcggcag
     2341 ctttgattcg ataagcagca tcgcctgttt caggctgtct atgtgtgact gttgagctgt
     2401 aacaagttgt ctcaggtgtt caatttcatg ttctagttgc tttgttttac tggtttcacc
     2461 tgttctatta ggtgttacat gctgttcatc tgttacattg tcgatctgtt catggtgaac
     2521 agctttgaat gcaccaaaaa ctcgtaaaag ctctgatgta tctatctttt ttacaccgtt
     2581 ttcatctgtg catatggaca gttttccctt tgatatgtaa cggtgaacag ttgttctact
     2641 tttgtttgtt agtcttgatg cttcactgat agatacaaga gccataagaa cctcagatcc
     2701 ttccgtattt agccagtatg ttctctagtg tggttcgttg tttttgcgtg agccatgaga
     2761 acgaaccatt gagatcatac ttactttgca tgtcactcaa aaattttgcc tcaaaactgg
     2821 tgagctgaat ttttgcagtt aaagcatcgt gtagtgtttt tcttagtccg ttatgtaggt
     2881 aggaatctga tgtaatggtt gttggtattt tgtcaccatt catttttatc tggttgttct
     2941 caagttcggt tacgagatcc atttgtctat ctagttcaac ttggaaaatc aacgtatcag
     3001 tcgggcggcc tcgcttatca accaccaatt tcatattgct gtaagtgttt aaatctttac
     3061 ttattggttt caaaacccat tggttaagcc ttttaaactc atggtagtta ttttcaagca
     3121 ttaacatgaa cttaaattca tcaaggctaa tctctatatt tgccttgtga gttttctttt
     3181 gtgttagttc ttttaataac cactcataaa tcctcataga gtatttgttt tcaaaagact
     3241 taacatgttc cagattatat tttatgaatt tttttaactg gaaaagataa tgcaatatct
     3301 cttcactaaa aactaattct aatttttcgc ttgagaactt ggcatagttt gtccactgga
     3361 aaatctcaaa gcctttaacc aaaggattcc tgatttccac agttctcgtc atcagctctc
     3421 tggttgcttt agctaataca ccataagcat tttccctact gatgttcatc atctgagcgt
     3481 attggttata agtgaacgat accgtccgtt ctttccttgt agggttttca atcgtggggt
     3541 tgagtagtgc cacacagcat aaaattagct tggtttcatg ctccgttaag tcatagcgac
     3601 taatcgctag ttcatttgct ttgaaaacaa ctaattcaga catacatctc aattggtcta
     3661 ggtgatttta atcactatac caattgagat gggctagtca atgataatta ctagtccttt
     3721 tcccgggaga tctgggtatc tgtaaattct gctagacctt tgctggaaaa cttgtaaatt
     3781 ctgctagacc ctctgtaaat tccgctagac ctttgtgtgt tttttttgtt tatattcaag
     3841 tggttataat ttatagaata aagaaagaat aaaaaaagat aaaaagaata gatcccagcc
     3901 ctgtgtataa ctcactactt tagtcagttc cgcagtatta caaaaggatg tcgcaaacgc
     3961 tgtttgctcc tctacaaaac agaccttaaa accctaaagg cttaagtagc accctcgcaa
     4021 gctcgggcaa atcgctgaat attccttttg tctccgacca tcaggcacct gagtcgctgt
     4081 ctttttcgtg acattcagtt cgctgcgctc acggctctgg cagtgaatgg gggtaaatgg
     4141 cactacaggc gccttttatg gattcatgca aggaaactac ccataataca agaaaagccc
     4201 gtcacgggct tctcagggcg ttttatggcg ggtctgctat gtggtgctat ctgacttttt
     4261 gctgttcagc agttcctgcc ctctgatttt ccagtctgac cacttcggat tatcccgtga
     4321 caggtcattc agactggcta atgcacccag taaggcagcg gtatcatcaa caggcttacc
     4381 cgtcttactg tccctagtgc ttggattctc accaataaaa aacgcccggc ggcaaccgag
     4441 cgttctgaac aaatccagat ggagttctga ggtctactag tgcttggatt ctcaccaata
     4501 aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc tgaggtcatt
     4561 actggatcta tcaacaggag tccaagcgag ctcgatatca aatcagtcct gctcctcggc
     4621 cacgaagtgc acgcagttgc cggccgggtc gcgcagggcg aactcccgcc cccacggctg
     4681 ctcgccgatc tcggtcatgg ccggcccgga ggcgtcccgg aagttcgtgg acacgacctc
     4741 cgaccactcg gcgtacagct cgtccaggcc gcgcacccac acccaggcca gggtgttgtc
     4801 cggcaccacc tggtcctgga ccgcgctgat gaacagggtc acgtcgtccc ggaccacacc
     4861 ggcgaagtcg tcctccacga agtcccggga gaacccgagc cggtcggtcc agaactcgac
     4921 cgctccggcg acgtcgcgcg cggtgagcac cggaacggca ctggtcaact tggccatact
     4981 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     5041 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     5101 gccacctgca tcgatttatt atgacaactt gacggctaca tcattcactt tttcttcaca
     5161 accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaataccc gcgagaagta
     5221 gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtggtgct
     5281 caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga cgctaatccc
     5341 taactgctgg cggaaaagat gtgacagacg cgacggcgac aagcaaacat gctgtgcgac
     5401 gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg
     5461 tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagtaa
     5521 caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc
     5581 gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa
     5641 gaaccccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt aggcgcgcgg
     5701 acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac cgtagtgatg
     5761 aatctctcct ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt ctcgtccctg
     5821 atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag
     5881 cggtcggtcg ataaaaaaat cgagataacc gttggcctca atcggcgtta aacccgccac
     5941 cagatgggca ttaaacgagt atcccggcag caggggatca ttttgcgctt cagccatact
     6001 tttcatactc ccgccattca gagaagaaac caattgtcca tattgcatca gacattgccg
     6061 tcactgcgtc ttttactggc tcttctcgct aaccaaaccg gtaaccccgc ttattaaaag
     6121 cattctgtaa caaagcggga ccaaagccat gacaaaaacg cgtaacaaaa gtgtctataa
     6181 tcacggcaga aaagtccaca ttgattattt gcacggcgtc acactttgct atgccatagc
     6241 atttttatcc ataagattag cggatcctac ctgacgcttt ttatcgcaac tctctactgt
     6301 ttctccatac ccgttttttt gggaattcga gctctaagga ggttataaaa aatggccaaa
     6361 gaagacaata ttgaaatgca aggtaccgtt cttgaaacgt tgcctaatac catgttccgc
     6421 gtagagttag aaaacggtca cgtggttact gcacacatct ccggtaaaat gcgcaaaaac
     6481 tacatccgca tcctgacggg cgacaaagtg actgttgaac tgaccccgta cgacctgagc
     6541 aaaggccgca ttgtcttccg tagtcgctga ctcgagtaag gatctccagg catcaaataa
     6601 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
     6661 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatacctagg
     6721 gatatattcc gcttcctcgc tcactgactc gctacgctcg gtcgttcgac tgcggcgagc
     6781 ggaaatggct tacgaacggg gcggagattt cctggaagat gccaggaaga tacttaacag
     6841 ggaagtgaga gggccgcggc aaagccgttt ttccataggc tccgcccccc tgacaagcat
     6901 cacgaaatct gacgctcaaa tcagtggtgg cgaaacccga caggactata aagataccag
     6961 gcgtttcccc ctggcggctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt
     7021 gtcattccgc tgttatggcc gcgtttgtct cattccacgc ctgacactca gttccgggta
     7081 ggcagttcgc tccaagctgg actgtatgca cgaacccccc gttcagtccg acagctgcgc
     7141 cttatccggt aactatcgtc ttgagtccaa cccggaaaga catgcaaaag cacaactggc
     7201 agcagccact ggtaattgat ttagaggagt tagtcttgaa gtcatgcgcc ggttaaggct
     7261 aaactgaaag gacaagtttt ggtgactgcg ctcctccaag ccagttacct cggttcaaag
     7321 agttggtagc tcagagaacc ttcgaaaaac cgccctgcaa ggcggttttt tcgttttcag
     7381 agcaagagat tacgcgcaga ccaaaacgat ctcaagaaga tcatcttatt aatcagataa
     7441 aatatttcta gatttcagtg caatttatct cttcaaatgt agcacctgaa gtcagcccca
     7501 tacgatataa gttgttacta gtgcttggat tctcaccaat aaaaaacgcc cggcggcaac
     7561 cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga
     7621 gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcatcgca gtactactag
     7681 tgcttggatt ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca
     7741 gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag ctctcgaacc
     7801 ccagagtccc gcttatttgc cgactacctt ggtgatctcg cctttcacgt agtggacaaa
     7861 ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct tcttgtccaa gataagcctg
     7921 tctagcttca agtatgacgg gctgatactg ggccggcagg cgctccattg cccagtcggc
     7981 agcgacatcc ttcggcgcga ttttgccggt tactgcgctg taccaaatgc gggacaacgt
     8041 aagcactaca tttcgctcat cgccagccca gtcgggcggc gagttccata gcgttaaggt
     8101 ttcatttagc gcctcaaata gatcctgttc aggaaccgga tcaaagagtt cctccgccgc
     8161 tggacctacc aaggcaacgc tatgttctct tgcttttgtc agcaagatag ccagatcaat
     8221 gtcgatcgtg gctggctcga agatacctgc aagaatgtca ttgcgctgcc attctccaaa
     8281 ttgcagttcg cgcttagctg gataacgcca cggaatgatg tcgtcgtgca caacaatggt
     8341 gacttctaca gcgcggagaa tctcgctctc tccaggggaa gccgaagttt ccaaaaggtc
     8401 gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa ctagcaaatc
     8461 aatatcactg tgtggcttca ggccgccatc cactgcggag ccgtacaaat gtacggccag
     8521 caacgtcggt tcgagatggc gctcgatggc gccaactacc tctgatagtt gagtcgatac
     8581 ttcggtgatc accgcttccc tcataatgtt taactttgtt ttagggcgac tgccctgctg
     8641 cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc atggcgtaac gcgcttgctg
     8701 cttggatgcc cgaggcatag actgtacccc aaaaaaacag tcatagcaag ccatgaaaac
     8761 cgccactgca ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg
     8821 catgcgaaac gatcctcatc ctgtctcttg atcagatctt gatcccctgc gccatcagat
     8881 ccttggcggc aagaaagcca tccagtttac tttgcagggc ttcccaacct taccagaggg
     8941 cgccccagct ggcaattccg acgtcgacgt cttaagaccc actttcacat ttaagttgtt
     9001 tttctaatcc gcatatgatc aattcaaggc cgaataagaa ggctggctct gcaccttggt
     9061 gatcaaataa ttcgatagct tgtcgtaata atggcggcat actatcagta gtaggtgttt
     9121 ccctttcttc tttagcgact tgatgctctt gatcttccaa tacgcaacct aaagtaaaat
     9181 gccccacagc gctgagtgca tataatgcat tctctagtga aaaaccttgt tggcataaaa
     9241 aggctaattg attttcgaga gtttcatact gtttttctgt aggccgtgta cctaaatgta
     9301 cttttgctcc atcgcgatga cttagtaaag cacatctaaa acttttagcg ttattacgta
     9361 aaaaatcttg ccagctttcc ccttctaaag ggcaaaagtg agtatggtgc ctatctaaca
     9421 tctcaatggc taaggcgtcg agcaaagccc gcttattttt tacatgccaa tacaatgtag
     9481 gctgctctac acctagcttc tgggcgagtt tacgggttgt taaaccttcg attccgacct
     9541 cattaagcag ctctaatgcg ctgttaatca ctttactttt atctaatcta gacatcatta
     9601 attcctaatt ttt
//
