LOCUS       sequence-267961-        8914 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FANCF_-_22647294.23-P1P2 sgRNA in pCR1068.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8914)
  AUTHORS   Richardson CD, Kazane KR, Feng SJ, Zelin E, Bray NL, Schafer AJ, 
            Floor SN, Corn JE
  TITLE     CRISPR-Cas9 genome editing in human cells occurs via the Fanconi 
            anemia pathway.
  JOURNAL   Nat Genet. 2018 Aug;50(8):1132-1139. doi: 10.1038/s41588-018-0174-0.
            Epub 2018 Jul 27.
  PUBMED    30054595
REFERENCE   2  (bases 1 to 8914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41588-018-0174-0"; journalName: "Nat Genet"; date: 
            "2018-08"; volume: "50"; issue: "8"; pages: "1132-1139"
COMMENT     Sequence Label: pCR1178
FEATURES             Location/Qualifiers
     source          1..8914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    106..223
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    complement(484..517)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     521..541
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     promoter        794..1005
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     intron          1006..1944
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1901..1921
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1965..2561
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1965..1984)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2461..2481
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             2571..2624
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      2631..2640
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2637..2639,2640..2642,2643..3356)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2682..2703)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2943..2962)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3290..3311
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(3376..3409)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    3465..4053
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3518..3538)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3936..3947)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4056..4072)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4057..4073)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4582..4762
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(4824..5412)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4904..4923)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5583..6374,6375..6443))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6206..6225
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6444..6548)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6623..6642)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        6814..7193
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7195..7393
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7348..7368
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             7411..7591
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7638..7763
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8260..8493
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8678..8722
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
       61 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
      121 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
      181 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
      241 cagcagagat ccagtttggt tagtaccggg cccgccctgc aggtctagag atccgacgcg
      301 ccatctctag gcccgcgccg gccccctcgc acggacttgt gggagaagct cggctactcc
      361 cctgccccgg ttaatttgca tataatattt cctagtaact atagaggctt aatgtgcgat
      421 aaaagacaga taatctgttc tttttaatac tagctacatt ttacatgata ggcttggatt
      481 tctataactt cgtatagcat acattatacg aagttataaa cagcacaaaa ggaaactcac
      541 cctaactgta aagtaattgt gtgttttgag actataagta tcccttggag aaccaccttg
      601 ttgggggtcc caggtgctga cgtgtttaag agctaagctg gaaacagcat agcaagttta
      661 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttctcg
      721 agtactagga tccattaggc ggccgccctg cagggtggat aaccgtatta ccgccatgca
      781 tgtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
      841 gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
      901 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
      961 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt
     1021 ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc
     1081 acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt
     1141 tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg
     1201 cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat
     1261 aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat
     1321 agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc
     1381 ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg
     1441 ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc
     1501 gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg
     1561 tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg
     1621 cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca
     1681 gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc
     1741 tcgcgctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt
     1801 ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc
     1861 ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt
     1921 caaagttttt ttcttccatt tcaggtgtcg tgagctagcc caccatgacc gagtacaagc
     1981 ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg
     2041 cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg
     2101 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     2161 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     2221 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     2281 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     2341 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     2401 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     2461 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     2521 aaggaccgcg cacctggtgc atgacccgca agcccggtgc cggatcggga gagggcagag
     2581 gaagtctgct aacatgcggt gacgtcgagg agaatcctgg cccaccggtc gccaccatgg
     2641 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     2701 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     2761 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc ggtgccctgg cccaccctcg
     2821 tgaccaccct gacccatggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     2881 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     2941 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     3001 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     3061 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     3121 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     3181 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     3241 tgagcaccca gtccaagctg agtaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     3301 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaagaat
     3361 tcgtcgaggg acctaataac ttcgtatagc atacattata cgaagttata catgtttaag
     3421 ggttccggtt ccactaggta caattcgata tcaagcttat cgataatcaa cctctggatt
     3481 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     3541 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     3601 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     3661 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     3721 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     3781 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     3841 ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct
     3901 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     3961 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     4021 cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgatcgagac
     4081 ctagaaaaac atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc
     4141 tggctagaag cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta
     4201 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     4261 ctggaagggc taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac
     4321 acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca
     4381 ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc
     4441 aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg
     4501 gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca catggcccga
     4561 gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     4621 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     4681 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     4741 tcagtgtgga aaatctctag cagcatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     4801 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     4861 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     4921 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     4981 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     5041 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     5101 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     5161 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     5221 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     5281 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     5341 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     5401 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     5461 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     5521 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     5581 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     5641 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     5701 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     5761 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     5821 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     5881 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     5941 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     6001 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     6061 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     6121 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     6181 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     6241 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     6301 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     6361 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     6421 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     6481 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     6541 gttccgcgca catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg
     6601 atcccctatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc
     6661 tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa
     6721 caaggcaagg cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc
     6781 tgcttcgcga tgtacgggcc agatatacgc gttgacattg attattgact agttattaat
     6841 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
     6901 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
     6961 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
     7021 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
     7081 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat
     7141 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
     7201 ggttttggca gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
     7261 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
     7321 aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg
     7381 tctatataag cagcgcgttt tgcctgtact gggtctctct ggttagacca gatctgagcc
     7441 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     7501 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     7561 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
     7621 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     7681 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     7741 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     7801 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     7861 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     7921 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     7981 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     8041 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     8101 cggccggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     8161 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     8221 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     8281 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     8341 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     8401 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     8461 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     8521 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     8581 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     8641 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     8701 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     8761 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     8821 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     8881 aaccccgagg ggacccgaca ggcccgaagg aata
//
