LOCUS       sequence-267980-        1008 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reporter plasmid expressing the ER-located RFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Dehler CE, Lester K, Della Pelle G, Jouneau L, Houel A, Collins C, 
            Dovgan T, Machat R, Zou J, Boudinot P, Martin SAM, Collet B
  TITLE     Viral Resistance and IFN Signaling in STAT2 Knockout Fish Cells.
  JOURNAL   J Immunol. 2019 Jul 15;203(2):465-475. doi: 
            10.4049/jimmunol.1801376. Epub 2019 May 29.
  PUBMED    31142600
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.4049/jimmunol.1801376"; journalName: "J Immunol"; date: 
            "2019-07-15- 15"; volume: "203"; issue: "2"; pages: "465-475"
COMMENT     Sequence Label: pcDNA3.1-Hyg-RFPKDEL
FEATURES             Location/Qualifiers
     source          1..1008
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..24
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5..23
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     5'UTR           42..84
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     CDS             93..182
                     /codon_start=1
                     /product="signal sequence from bovine prolactin"
                     /label=prolactin signal sequence
                     /translation="MDSKGSSQKGSRLLLLLVVSNLLLCQGVVS"
     CDS             195..869
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             195..869
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(325..343)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     593..612
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     787..810
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(929..946)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 tcaataatac gactcactat agggagaccc aagctggcta gcttgttctt tttgcagaag
       61 ctcagaataa acgctcaact ttggcagata ccatggacag caaaggttcg tcgcagaaag
      121 ggtcccgcct gctcctgctg ctggtggtgt caaatctact cttgtgccag ggtgtggtct
      181 cctgcagtcg acttatggcc tcctccgagg acgtcatcaa ggagttcatg cgcttcaagg
      241 tgcgcatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
      301 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggcggc cccctgccct
      361 tcgcctggga catcctgtcc cctcagttcc agtacggctc caaggcctac gtgaagcacc
      421 ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg
      481 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg
      541 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa
      601 tgcagaagaa gaccatgggc tgggaggcct ccaccgagcg gatgtacccc gaggacggcg
      661 ccctgaaggg cgagatcaag atgaggctga agctgaagga cggcggccac tacgacgccg
      721 aggtcaagac cacctacatg gccaagaagc ccgtgcagct gcccggcgcc tacaagaccg
      781 acatcaagct ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgagc
      841 gcgccgaggg ccgccactcc accggcgcca aagatgagct ctaaggatcc gagctcggta
      901 ccaagcttaa gtttaaaccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
      961 gttgtttgcc cctccccgtg ccttccttga ccctggaagg tgccctcc
//
