LOCUS       sequence-267981-        1025 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Reporter plasmid expressing the ER-located RFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Dehler CE, Lester K, Della Pelle G, Jouneau L, Houel A, Collins C, 
            Dovgan T, Machat R, Zou J, Boudinot P, Martin SAM, Collet B
  TITLE     Viral Resistance and IFN Signaling in STAT2 Knockout Fish Cells.
  JOURNAL   J Immunol. 2019 Jul 15;203(2):465-475. doi: 
            10.4049/jimmunol.1801376. Epub 2019 May 29.
  PUBMED    31142600
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.4049/jimmunol.1801376"; journalName: "J Immunol"; date: 
            "2019-07-15- 15"; volume: "203"; issue: "2"; pages: "465-475"
COMMENT     Sequence Label: pcDNA3.1-Hyg-RFPKDEL
FEATURES             Location/Qualifiers
     source          1..1025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(54..728)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             complement(54..728)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(113..136)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(311..330)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     580..598
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(741..830)
                     /codon_start=1
                     /product="signal sequence from bovine prolactin"
                     /label=prolactin signal sequence
                     /translation="MDSKGSSQKGSRLLLLLVVSNLLLCQGVVS"
     5'UTR           complement(839..881)
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     primer_bind     complement(899..918)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(900..918)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(963..1014)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(992..1012)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ggggaaggtt acttaagctt ggtaccgagc tcggatcctt agagctcatc tttggcgccg
       61 gtggagtggc ggccctcggc gcgctcgtac tgttccacga tggtgtagtc ctcgttgtgg
      121 gaggtgatgt ccagcttgat gtcggtcttg taggcgccgg gcagctgcac gggcttcttg
      181 gccatgtagg tggtcttgac ctcggcgtcg tagtggccgc cgtccttcag cttcagcctc
      241 atcttgatct cgcccttcag ggcgccgtcc tcggggtaca tccgctcggt ggaggcctcc
      301 cagcccatgg tcttcttctg cattacgggg ccgtcggagg ggaagttggt gccgcgcagc
      361 ttcaccttgt agatgaactc gccgtcctgc agggaggagt cctgggtcac ggtcaccacg
      421 ccgccgtcct cgaagttcat cacgcgctcc cacttgaagc cctcggggaa ggacagcttc
      481 aagtagtcgg ggatgtcggc ggggtgcttc acgtaggcct tggagccgta ctggaactga
      541 ggggacagga tgtcccaggc gaagggcagg gggccgccct tggtcacctt cagcttggcg
      601 gtctgggtgc cctcgtaggg gcggccctcg ccctcgccct cgatctcgaa ctcgtggccg
      661 ttcacggagc cctccatgcg caccttgaag cgcatgaact ccttgatgac gtcctcggag
      721 gaggccataa gtcgactgca ggagaccaca ccctggcaca agagtagatt tgacaccacc
      781 agcagcagga gcaggcggga ccctttctgc gacgaacctt tgctgtccat ggtatctgcc
      841 aaagttgagc gtttattctg agcttctgca aaaagaacaa gctagccagc ttgggtctcc
      901 ctatagtgag tcgtattaat ttcgataagc cagtaagcag tgggttctct agttagccag
      961 agagctctgc ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcatgggg
     1021 cggaa
//
