LOCUS       sequence-267982-        1441 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  stable GFP reporter to monitor calcineurin activity via 
            calcineurin-dependent response element (CDRE) in S. cerevisiae.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Diessl , J.,  Nandy, A., Schug, C., Habernig, L.,, Buttner, S.
  TITLE     Stable and destabilized GFP reporters to monitor calcineurin 
            activity in Saccharomyces cerevisiae.
  JOURNAL   Microbial Cell, 2020: in press
REFERENCE   2  (bases 1 to 1441)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microbial 
            Cell, 2020: in press"
COMMENT     Sequence Label: pAMS366-4xCDRE-GFP
FEATURES             Location/Qualifiers
     source          1..1441
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             628..1344
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 gtatagaccg tggatgatgt ggtctctaca ggatctgaca ttattattgt tggaagagga
       61 ctatttgcaa agggaaggga tgctaaggta gagggtgaac gttacagaaa agcaggctgg
      121 gaagcatatt tgagaagatg cggccagcaa aactaaaaaa ctgtattata agtaaatgca
      181 tgtatactaa actcacaaat tagagcttca atttaattat atcagttatt accctcgagc
      241 aagcgcacag ccaccgactg gtgtcgagca agcgcacagc caccgactgg tgtcgaggtc
      301 gagcaagcgc acagccaccg actggtgtcg aggtcgagca agcgcacagc caccgactgg
      361 tgtcgaggtc gagcagatcc gccaggcgtg tatatagcgt ggatggccag gcaactttag
      421 tgctgacaca tacaggcata tatatatgtg tgcgacgaca catgatcata tggcatgcat
      481 gtgctctgta tgtatataaa actcttgttt tcttcttttc tctaaatatt ctttccttat
      541 acattaggtc ctttgtagca taaattacta tacttctata gacacgcaaa cacaaataca
      601 cacactaaat taataatgac cggatccatg tctaaaggtg aagaattatt cactggtgtt
      661 gtcccaattt tggttgaatt agatggtgat gttaatggtc acaaattttc tgtctccggt
      721 gaaggtgaag gtgatgctac ttacggtaaa ttgaccttaa aatttatttg tactactggt
      781 aaattgccag ttccatggcc aaccttagtc actactttcg gttatggtgt tcaatgtttt
      841 gcgagatacc cagatcatat gaaacaacat gactttttca agtctgccat gccagaaggt
      901 tatgttcaag aaagaactat ttttttcaaa gatgacggta actacaagac cagagctgaa
      961 gtcaagtttg aaggtgatac cttagttaat agaatcgaat taaaaggtat tgattttaaa
     1021 gaagatggta acattttagg tcacaaattg gaatacaact ataactctca caatgtttac
     1081 atcatggctg acaaacaaaa gaatggtatc aaagttaact tcaaaattag acacaacatt
     1141 gaagatggtt ctgttcaatt agctgaccat tatcaacaaa atactccaat tggtgatggt
     1201 ccagtcttgt taccagacaa ccattactta tccactcaat ctgccttatc caaagatcca
     1261 aacgaaaaga gagaccacat ggtcttgtta gaatttgtta ctgctgctgg tattacccat
     1321 ggtatggatg aattgtacaa ataagagctc ctgcactgga tggtggcgct ggatggtaag
     1381 ccgctggcaa gcggtgaagt gcctctggat gtcgctccac aaggtaaaca gttgatgaac
     1441 t
//
