LOCUS       sequence-267983-       10157 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express hHOXA13 and EGFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10157)
  AUTHORS   George JW, Fan H, Johnson B, Carpenter TJ, Foy KK, Chatterjee A, 
            Patterson AL, Koeman J, Adams M, Madaj ZB, Chesla D, Marsh EE, 
            Triche TJ, Shen H, Teixeira JM
  TITLE     Integrated Epigenome, Exome, and Transcriptome Analyses Reveal 
            Molecular Subtypes and Homeotic Transformation in Uterine Fibroids.
  JOURNAL   Cell Rep. 2019 Dec 17;29(12):4069-4085.e6. doi: 
            10.1016/j.celrep.2019.11.077.
  PUBMED    31851934
REFERENCE   2  (bases 1 to 10157)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.11"; journalName: "Cell Rep"; date: 
            "2019-12-17- 17"; volume: "29"; issue: "12"; pages: "4069-4085.e6"
COMMENT     Sequence Label: LV-hHOXA13
FEATURES             Location/Qualifiers
     source          1..10157
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        1959..2244
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        2246..2520
                     /label=chicken beta-actin promoter
     intron          2521..2748
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     protein_bind    2753..2777
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3951..3975)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3993..4581
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4046..4066)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4464..4475)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     enhancer        4611..4990
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4991..5194
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5145..5165
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      5221..5230
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5227..5229,5230..5232,5233..5943)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5227..5229,5230..5232,5233..5943)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5272..5293)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5533..5552)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5880..5901
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             5953..6006
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             6007..6606
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6007..6026)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6503..6523
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6678..6911
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6983..7104
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7020..7039)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7074..7093
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7144..7279
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7299..7318)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7300..7318)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7328..7345)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7328..7344)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7337..7359)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7486..7941
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7573..7592)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7783..7804
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7967..8071
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8072..8140,8141..8932)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8290..8309)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9103..9691
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9592..9611
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9845..9862
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9979..10000
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10015..10020,10021..10038,10039..10045)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10053..10069
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10058..10080
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10077..10093
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10077..10093
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10112..10132
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10114..10132
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgcg ttacataact tacggtaaat
     1981 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaatagt aacgccaata
     2041 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     2101 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     2161 gcctggcatt gtgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     2221 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
     2281 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
     2341 gcgatggggg cggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg
     2401 ggcggggcga ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt
     2461 ccttttatgg cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg
     2521 ggagtcgctg cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg
     2581 ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct
     2641 ccgggctgta attagctgag caagaggtaa gggtttaagg gatggttggt tggtggggta
     2701 ttaatgttta attacctgga gcacctgcct gaaatcactt tttttcaggt tggcaagttt
     2761 gtacaaaaaa gcaggctgcc accatgacag cctccgtgct cctccacccc cgctggatcg
     2821 agcccaccgt catgtttctc tacgacaacg gcggcggcct ggtggccgac gagctcaaca
     2881 agaacatgga aggggcggcg gcggctgcag cagcggctgc agcggcggcg gctgccgggg
     2941 ccgggggcgg gggcttcccc cacccggcgg ctgcggcggc agggggcaac ttctcggtgg
     3001 cggcggcggc cgcggctgcg gcggcggccg cggccaacca gtgccgcaac ctgatggcgc
     3061 acccggcgcc cttggcgcca ggagccgcgt ccgcctacag cagcgccccc ggggaggcgc
     3121 ccccgtcggc tgccgccgct gctgccgcgg ctgccgctgc agccgccgcc gccgccgccg
     3181 cgtcgtcctc gggaggtccc ggcccggcgg gcccggcggg cgcagaggcc gccaagcaat
     3241 gcagcccctg ctcggcagcg gcgcagagct cgtcggggcc cgcggcgctg ccctatggct
     3301 acttcggcag cggctactac ccgtgcgccc gcatgggccc gcaccccaac gccatcaagt
     3361 cgtgcgcgca gcccgcctcg gccgccgccg ccgccgcctt cgcggacaag tacatggata
     3421 ccgccggccc agctgccgag gagttcagct cccgcgctaa ggagttcgcc ttctaccacc
     3481 agggctacgc agccgggcct taccaccacc atcagcccat gcctggctac ctggatatgc
     3541 cagtggtgcc gggcctcggg ggccccggcg agtcgcgcca cgaacccttg ggtcttccca
     3601 tggaaagcta ccagccctgg gcgctgccca acggctggaa cggccaaatg tactgcccca
     3661 aagagcaggc gcagcctccc cacctctgga agtccactct gcccgacgtg gtctcccatc
     3721 cctcggatgc cagctcctat aggaggggga gaaagaagcg cgtgccttat accaaggtgc
     3781 aattaaaaga acttgaacgg gaatacgcca cgaataaatt cattactaag gacaaacgga
     3841 ggcggatatc agccacgacg aatctctctg agcggcaggt cacaatctgg ttccagaaca
     3901 ggagggttaa agagaaaaaa gtcatcaaca aactgaaaac cactagttaa acccagcttt
     3961 cttgtacaaa gtggtgataa tcgaattccg ataatcaacc tctggattac aaaatttgtg
     4021 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     4081 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     4141 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     4201 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     4261 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     4321 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     4381 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     4441 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     4501 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     4561 ccctttgggc cgcctccccg catcgggaat tcccgcggtt cgaacgcgtt gacattgatt
     4621 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     4681 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     4741 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     4801 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     4861 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     4921 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     4981 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     5041 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     5101 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     5161 gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc
     5221 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     5281 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     5341 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     5401 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     5461 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     5521 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     5581 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     5641 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     5701 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     5761 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     5821 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     5881 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     5941 aagggctccg gagagggcag gggaagtctt ctaacatgcg gggacgtgga ggaaaatccc
     6001 ggccccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     6061 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     6121 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     6181 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     6241 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     6301 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     6361 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     6421 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     6481 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     6541 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     6601 gcctgaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     6661 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     6721 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     6781 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     6841 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     6901 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     6961 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     7021 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     7081 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     7141 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     7201 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     7261 ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg
     7321 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     7381 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     7441 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
     7501 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     7561 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     7621 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     7681 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     7741 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     7801 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     7861 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     7921 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat
     7981 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     8041 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     8101 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     8161 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     8221 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     8281 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     8341 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     8401 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     8461 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     8521 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     8581 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     8641 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     8701 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     8761 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     8821 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     8881 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     8941 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     9001 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     9061 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     9121 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     9181 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     9241 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     9301 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     9361 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     9421 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     9481 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gagagaaagg cggacaggta
     9541 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     9601 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     9661 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     9721 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     9781 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     9841 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     9901 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     9961 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
    10021 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
    10081 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
    10141 gctggagctg caagctt
//
