LOCUS       sequence-267985-        1287 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a Tomato-targeting sgRNA, Cas9, and Cre-recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Hollstein PE, Eichner LJ, Brun SN, Kamireddy A, Svensson RU, Vera 
            LI, Ross DS, Rymoff TJ, Hutchins A, Galvez HM, Williams AE, 
            Shokhirev MN, Screaton RA, Berdeaux R, Shaw RJ
  TITLE     The AMPK-Related Kinases SIK1 and SIK3 Mediate Key Tumor-Suppressive
            Effects of LKB1 in NSCLC.
  JOURNAL   Cancer Discov. 2019 Nov;9(11):1606-1627. doi: 
            10.1158/2159-8290.CD-18-1261. Epub 2019 Jul 26.
  PUBMED    31350328
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-18-1261"; journalName: "Cancer Discov"; date: 
            "2019-11"; volume: "9"; issue: "11"; pages: "1606-1627"
COMMENT     Sequence Label: pSECC-sgTom
FEATURES             Location/Qualifiers
     source          1..1287
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        69..144
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    192..309
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        408..619
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      664..673
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             673..696
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             703..723
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ccgtattcga ttcttggctt tatatatctt gtggaaggac gaaacaccgg ccacgagttc
       61 gagatcgagt tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt
      121 gaaaaagtgg caccgagtcg gtgctttttt gaattctaga tcttgagaca aatggcagta
      181 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
      241 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
      301 caaaattttc gggtttatta cagggacagc agagatccac tttggcgccg gcaaaccgct
      361 gttcctagga atcccgaggc cttcgagtgg ctccggtgcc cgtcagtggg cagagcgcac
      421 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag
      481 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
      541 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
      601 gtttgccgcc agaacacagg tgtcgtgacg cgggacccga cattagcgct acagcttaag
      661 cgggccacca tggattacaa agacgatgac gataagatgg ccccaaagaa gaagcggaag
      721 gtcggtatcc acggagtccc agcagccgac aagaagtaca gcatcggcct ggacatcggc
      781 accaactctg tgggctgggc cgtgatcacc gacgagtaca aagtgcccag caagaaattc
      841 aaggtgctgg gcaacaccga ccggcacagc atcaagaaga acctgatcgg agccctgctg
      901 ttcgacagcg gcgaaacagc cgaggccacc cggctgagag aaccgccaga agagatacac
      961 cagacggaag aaccggatct gctatctgca agagatcttc agcaacgaga tggccaagtg
     1021 gacgacagct tcttcacaga ctgagagtct tcctggtgga agaggataga gcacgagcgg
     1081 caccccatct cgcacatcgt ggacgaggtg gctacacgag agtaccccac catctaccac
     1141 tggagaaaga aactgngann nacgacagnn gactggcggc tgatctatct gnctncnaat
     1201 gatacaagtt cgnnaattct gatcaggcac tgactcgaca cagcgacnga acagccgtgt
     1261 tcncatcatc cngctgtgtg tgatgca
//
