LOCUS       sequence-267987-         631 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a mouse LKB1-targeting sgRNA, Cas9, and Cre-recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Hollstein PE, Eichner LJ, Brun SN, Kamireddy A, Svensson RU, Vera 
            LI, Ross DS, Rymoff TJ, Hutchins A, Galvez HM, Williams AE, 
            Shokhirev MN, Screaton RA, Berdeaux R, Shaw RJ
  TITLE     The AMPK-Related Kinases SIK1 and SIK3 Mediate Key Tumor-Suppressive
            Effects of LKB1 in NSCLC.
  JOURNAL   Cancer Discov. 2019 Nov;9(11):1606-1627. doi: 
            10.1158/2159-8290.CD-18-1261. Epub 2019 Jul 26.
  PUBMED    31350328
REFERENCE   2  (bases 1 to 631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-18-1261"; journalName: "Cancer Discov"; date: 
            "2019-11"; volume: "9"; issue: "11"; pages: "1606-1627"
COMMENT     Sequence Label: pSECC-sgLKB1-A
FEATURES             Location/Qualifiers
     source          1..631
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        61..136
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    184..301
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cnttggggct ttatatatct tgtggaagga cgaaacaccg cgtacgagcc ctccccgagc
       61 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt
      121 ggcaccgagt cggtgctttt ttgaattcta gatcttgaga caaatggcag tattcatcca
      181 caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
      241 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
      301 tcgggtttat tacagggaca gcagagatcc actttggcgc cggcaaaccg ctgttcctag
      361 gaatcccgag gccttcgagt ggctccggtg cccgtcagtg ggcagagcgc acatcgccca
      421 cagtccccga gaagttgggg ggaggggtcg gcaattgacc cggtgcctag agaaggtggc
      481 gcagggtaaa ctggaaaagg gacgtcgtgt accgactccg ccgtggtgga gtgagaggtg
      541 gagagcagag aaacagaatg ggtgggcgtg gaaagtacgg gagggagggg gatgggccgg
      601 ggaagatgtg tggaggggcg gcggagggga g
//
