LOCUS       sequence-267990-        1248 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a mouse SIK1-targeting sgRNA, Cas9, and Cre-recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Hollstein PE, Eichner LJ, Brun SN, Kamireddy A, Svensson RU, Vera 
            LI, Ross DS, Rymoff TJ, Hutchins A, Galvez HM, Williams AE, 
            Shokhirev MN, Screaton RA, Berdeaux R, Shaw RJ
  TITLE     The AMPK-Related Kinases SIK1 and SIK3 Mediate Key Tumor-Suppressive
            Effects of LKB1 in NSCLC.
  JOURNAL   Cancer Discov. 2019 Nov;9(11):1606-1627. doi: 
            10.1158/2159-8290.CD-18-1261. Epub 2019 Jul 26.
  PUBMED    31350328
REFERENCE   2  (bases 1 to 1248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-18-1261"; journalName: "Cancer Discov"; date: 
            "2019-11"; volume: "9"; issue: "11"; pages: "1606-1627"
COMMENT     Sequence Label: pSECC-sgSIK1-B
FEATURES             Location/Qualifiers
     source          1..1248
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        71..146
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    194..311
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        410..621
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      666..675
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             675..698
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             705..725
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gccgtatgcg attcttggct ttatatatct tgtggaagga cgaaacaccg cgtcgtaaaa
       61 gcccacccgg gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac
      121 ttgaaaaagt ggcaccgagt cggtgctttt ttgaattcta gatcttgaga caaatggcag
      181 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
      241 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
      301 ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggcgc cggcaaaccg
      361 ctgttcctag gaatcccgag gccttcgagt ggctccggtg cccgtcagtg ggcagagcgc
      421 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac cggtgcctag
      481 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
      541 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
      601 gggtttgccg ccagaacaca ggtgtcgtga cgcgggaccc gacattagcg ctacagctta
      661 agcgggccac catggattac aaagacgatg acgataagat ggccccaaag aagaagcgga
      721 aggtcggtat ccacggagtc ccagcagccg acaagaagta cagcatcggc ctggacatcg
      781 gcaccaactc tgtgggctgg gccgtgatca ccgacgagta caagtgccca gcaagaaatt
      841 caaggtgctg ggcaacaccg accggcacag catcaagaag aacctgatcg gagccctgct
      901 gttcgacagc ggcgaaacag ccgaggccac ccggctgaga gaaccgccag aagagataca
      961 ccagacggaa gaaccggatc tgctatctgc agagatctca gcacgagatg gccaaggtgg
     1021 acgacagctt cttcacagac tgagagtctc ctgtgagaga taagaagcac gagcgcaccc
     1081 atctcgcaca tcgtgacgag gtgctaccac gagagtaccc acatctacca ctgaaaaaga
     1141 actgtgacgc acgacagccg acctgcgctg atctatctgc nnncccatga tcagtngnnn
     1201 nnntatcagg caactgaccn gacacacgcg ganggtgnnc acagatcc
//
