LOCUS       sequence-267997-        1261 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a mouse SIK3-targeting sgRNA, Cas9, and Cre-recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Hollstein PE, Eichner LJ, Brun SN, Kamireddy A, Svensson RU, Vera 
            LI, Ross DS, Rymoff TJ, Hutchins A, Galvez HM, Williams AE, 
            Shokhirev MN, Screaton RA, Berdeaux R, Shaw RJ
  TITLE     The AMPK-Related Kinases SIK1 and SIK3 Mediate Key Tumor-Suppressive
            Effects of LKB1 in NSCLC.
  JOURNAL   Cancer Discov. 2019 Nov;9(11):1606-1627. doi: 
            10.1158/2159-8290.CD-18-1261. Epub 2019 Jul 26.
  PUBMED    31350328
REFERENCE   2  (bases 1 to 1261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-18-1261"; journalName: "Cancer Discov"; date: 
            "2019-11"; volume: "9"; issue: "11"; pages: "1606-1627"
COMMENT     Sequence Label: pSECC-sgSIK3-B
FEATURES             Location/Qualifiers
     source          1..1261
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        74..149
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    197..314
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        413..624
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      669..678
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             678..701
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             708..728
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gccgtaattt cgatttcttg gctttatata tcttgtggaa ggacgaaaca ccgctcgtag
       61 tagccgatgc gaggttttag agctagaaat agcaagttaa aataaggcta gtccgttatc
      121 aacttgaaaa agtggcaccg agtcggtgct tttttgaatt ctagatcttg agacaaatgg
      181 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
      241 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
      301 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg cgccggcaaa
      361 ccgctgttcc taggaatccc gaggccttcg agtggctccg gtgcccgtca gtgggcagag
      421 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc
      481 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
      541 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
      601 aacgggtttg ccgccagaac acaggtgtcg tgacgcggga cccgacatta gcgctacagc
      661 ttaagcgggc caccatggat tacaaagacg atgacgataa gatggcccca aagaagaagc
      721 ggaaggtcgg tatccacgga gtcccagcag ccgacaagaa gtacagcatc ggcctggaca
      781 tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga gtacaaagtg cccagcaaga
      841 aattcaaggt gctgggcaac accgaccggc acagcatcaa gaagaacctg atcggagccc
      901 tgctgttcga cagcggcgaa acagccgagc cacccggctg agagaaccgc cagaagagat
      961 acaccagacg gaagaaccgg atctgctatc tgcagagatc ttcagcacga gatggccagg
     1021 tggacgacag cttcttccac agactgagag tcctctgtga agagatagag cacgagcgca
     1081 cccatctcgc acatcgtgac gagtgctaca cggaggagta ccccacatcn acactgaaag
     1141 aacctggtga cngcaccgac aggcgacctg cgctgattca tctgcnnncc caatgatcag
     1201 tngnnanttc tgatcagcac gtgaccgaca cggcgagtga acagaccgtt ctactatcag
     1261 a
//
