LOCUS       sequence-267998-        1258 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a mouse SIK3-targeting sgRNA, Cas9, and Cre-recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Hollstein PE, Eichner LJ, Brun SN, Kamireddy A, Svensson RU, Vera 
            LI, Ross DS, Rymoff TJ, Hutchins A, Galvez HM, Williams AE, 
            Shokhirev MN, Screaton RA, Berdeaux R, Shaw RJ
  TITLE     The AMPK-Related Kinases SIK1 and SIK3 Mediate Key Tumor-Suppressive
            Effects of LKB1 in NSCLC.
  JOURNAL   Cancer Discov. 2019 Nov;9(11):1606-1627. doi: 
            10.1158/2159-8290.CD-18-1261. Epub 2019 Jul 26.
  PUBMED    31350328
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-18-1261"; journalName: "Cancer Discov"; date: 
            "2019-11"; volume: "9"; issue: "11"; pages: "1606-1627"
COMMENT     Sequence Label: pSECC-sgSIK3-C
FEATURES             Location/Qualifiers
     source          1..1258
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        73..148
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    196..313
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        412..623
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      668..677
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             677..700
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             707..727
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gccggtatac gatttcttgg ctttatatat cttgtggaag gacgaaacac cgtggcagag
       61 aaggaagctc gagttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca
      121 acttgaaaaa gtggcaccga gtcggtgctt ttttgaattc tagatcttga gacaaatggc
      181 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
      241 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
      301 aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc gccggcaaac
      361 cgctgttcct aggaatcccg aggccttcga gtggctccgg tgcccgtcag tgggcagagc
      421 gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga accggtgcct
      481 agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc
      541 ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca
      601 acgggtttgc cgccagaaca caggtgtcgt gacgcgggac ccgacattag cgctacagct
      661 taagcgggcc accatggatt acaaagacga tgacgataag atggccccaa agaagaagcg
      721 gaaggtcggt atccacggag tcccagcagc cgacaagaag tacagcatcg gcctggacat
      781 cggcaccaac tctgtgggct gggccgtgat caccgacgag tacaaagtgc ccagcaagaa
      841 attcaaaggt gctgggcaac accgaccggc acagcatcaa gaagaaccct gatcggagcc
      901 ctgctgttcg acagcggcga aacagccgag gccacccggg ctgagagaac cgccagagag
      961 atacaccaga cggaagaccg gatctgctat ctgcaagaga tcttcagcac gagatggcca
     1021 agtggacgac agcttcttca cagactgaga gtccttcctg tgaagaggat agagcacgag
     1081 cgcacccatc tcgcacatcg tggacgaggt gctacacgag agtacccacc atctaccacc
     1141 tgaaagaant gnganncacc gacagngact ggcggctgat tctatctgnc ctnnncnnna
     1201 tcagttngnc attctgtatc aaggcaactg acccganaac gggaacggtg tgaacaaa
//
