LOCUS       sequence-268001-        1278 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a mouse MARK4-targeting sgRNA, Cas9, and Cre-recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Hollstein PE, Eichner LJ, Brun SN, Kamireddy A, Svensson RU, Vera 
            LI, Ross DS, Rymoff TJ, Hutchins A, Galvez HM, Williams AE, 
            Shokhirev MN, Screaton RA, Berdeaux R, Shaw RJ
  TITLE     The AMPK-Related Kinases SIK1 and SIK3 Mediate Key Tumor-Suppressive
            Effects of LKB1 in NSCLC.
  JOURNAL   Cancer Discov. 2019 Nov;9(11):1606-1627. doi: 
            10.1158/2159-8290.CD-18-1261. Epub 2019 Jul 26.
  PUBMED    31350328
REFERENCE   2  (bases 1 to 1278)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-18-1261"; journalName: "Cancer Discov"; date: 
            "2019-11"; volume: "9"; issue: "11"; pages: "1606-1627"
COMMENT     Sequence Label: pSECC-sgMARK4-B
FEATURES             Location/Qualifiers
     source          1..1278
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        70..145
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    193..310
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        409..620
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      665..674
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             674..697
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             704..724
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cnnattcgat ttcttggctt tatatatctt gtggaaggac gaaacaccgc ccgggcaacg
       61 atcgaaactg ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact
      121 tgaaaaagtg gcaccgagtc ggtgcttttt tgaattctag atcttgagac aaatggcagt
      181 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
      241 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
      301 tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc ggcaaaccgc
      361 tgttcctagg aatcccgagg ccttcgagtg gctccggtgc ccgtcagtgg gcagagcgca
      421 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
      481 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
      541 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
      601 ggtttgccgc cagaacacag gtgtcgtgac gcgggacccg acattagcgc tacagcttaa
      661 gcgggccacc atggattaca aagacgatga cgataagatg gccccaaaga agaagcggaa
      721 ggtcggtatc cacggagtcc cagcagccga caagaagtac agcatcgggc ctggacatcg
      781 gcaccaactc tgtgggctgg gccgtgatca ccgacgagta caaggtgccc agcaagaaat
      841 ttcaagggtg ctgggcaaca ccgaccggca cagcatcaag aagaaccctg atcggagccc
      901 tgctgttcga acagcggcga agcagccgag gccacccggc tgaagagaac cgccagaaga
      961 agatacacca gacggaagaa ccggatctgc tatctgcaga gatcttcggc aacgatatgg
     1021 ccaaggtgaa cgacgctctt cacagactga gatccttcct gtgaaacaga tacaaacacg
     1081 agcgacaccc atcctcagca gatcgtggac aagttgacta tacatagagg ccccacgtct
     1141 acgacgtaga cgagcctggt ggcacgaccc gactatgcta ctccgcttaa tgcaactnng
     1201 ccttagccag taatcannng ggnncnncca gatcgnnncc tgaacccgcc acaannnann
     1261 nggtcaccag caggtcta
//
