LOCUS       sequence-268002-        1264 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a Non-targeting sgRNA and Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Hollstein PE, Eichner LJ, Brun SN, Kamireddy A, Svensson RU, Vera 
            LI, Ross DS, Rymoff TJ, Hutchins A, Galvez HM, Williams AE, 
            Shokhirev MN, Screaton RA, Berdeaux R, Shaw RJ
  TITLE     The AMPK-Related Kinases SIK1 and SIK3 Mediate Key Tumor-Suppressive
            Effects of LKB1 in NSCLC.
  JOURNAL   Cancer Discov. 2019 Nov;9(11):1606-1627. doi: 
            10.1158/2159-8290.CD-18-1261. Epub 2019 Jul 26.
  PUBMED    31350328
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-18-1261"; journalName: "Cancer Discov"; date: 
            "2019-11"; volume: "9"; issue: "11"; pages: "1606-1627"
COMMENT     Sequence Label: pLenti-Blast-sgNT
FEATURES             Location/Qualifiers
     source          1..1264
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        77..152
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        214..425
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      444..453
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aaccttattt tcgattttct ttggctttat atatcttgtg gaaaggacga aacaccgcga
       61 ggtattcggc tccgcggttt tagagctaga aatagcaagt taaaataagg ctagtccgtt
      121 atcaacttga aaaagtggca ccgagtcggt gcttttttga attcgctagc taggtcttga
      181 aaggagtggg aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc
      241 ccgagaagtt ggggggaggg gtcggcaatt gatccggtgc ctagagaagg tggcgcgggg
      301 taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac
      361 cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa
      421 cacaggaccg gttctagagc gctgccacca tggacaagaa gtacagcatc ggcctggaca
      481 tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga gtacaaggtg cccagcaaga
      541 aattcaaggt gctgggcaac accgaccggc acagcatcaa gaagaacctg atcggagccc
      601 tgctgttcga cagcggcgaa acagccgagg ccacccggct gaagagaacc gccagaagaa
      661 gatacaccag acggaagaac cggatctgct atctgcaaga gatcttcagc aacgagatgg
      721 ccaaggtgga cgacagcttc ttccacagac tggaagagtc cttcctggtg gaagaggata
      781 agaagcacga gcggcacccc atcttcggca acatcgtgga cgaggtggcc taccacgaga
      841 agtaccccac catctaccac ctgagaaaga aactggtgga cagcaccgac aaggccgacc
      901 tgcggctgat ctatctggcc ctggcccaca tgatcaagtt ccggggccac ttcctgatcg
      961 agggcgacct gaaccccgac aacagcgacg tggacaagct tgttcatcca gctggtgcag
     1021 acctacaacc agcttgttcg aggaaacccc atcacgccag cggcgtgacg ccaagccatc
     1081 cttgtctgcc agactgacag agcagacgct ggaaattctg atcgcccagc tgccggcaga
     1141 agaagaattg cttgtttcga aactgattgc ctganctggc tgacccnctt ccagacactc
     1201 gactgccgag gatgcactgc agctggacca gggaacctag cagacgaacg aaaccgtgta
     1261 acaa
//
