LOCUS       sequence-268003-        1302 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a mouse SIK1-targeting sgRNA and Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1302)
  AUTHORS   Hollstein PE, Eichner LJ, Brun SN, Kamireddy A, Svensson RU, Vera 
            LI, Ross DS, Rymoff TJ, Hutchins A, Galvez HM, Williams AE, 
            Shokhirev MN, Screaton RA, Berdeaux R, Shaw RJ
  TITLE     The AMPK-Related Kinases SIK1 and SIK3 Mediate Key Tumor-Suppressive
            Effects of LKB1 in NSCLC.
  JOURNAL   Cancer Discov. 2019 Nov;9(11):1606-1627. doi: 
            10.1158/2159-8290.CD-18-1261. Epub 2019 Jul 26.
  PUBMED    31350328
REFERENCE   2  (bases 1 to 1302)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-18-1261"; journalName: "Cancer Discov"; date: 
            "2019-11"; volume: "9"; issue: "11"; pages: "1606-1627"
COMMENT     Sequence Label: pLenti-Blast-sgSIK1-B
FEATURES             Location/Qualifiers
     source          1..1302
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        75..150
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        212..423
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      442..451
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tanaaaattt tctatttctt ggctttatat atcttgtgga gggacgaaac accgcgtcgt
       61 aaaagcccac ccgggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat
      121 caacttgaaa aagtggcacc gagtcggtgc ttttttgaat tcgctagcta ggtcttgaaa
      181 ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
      241 gagaagttgg ggggaggggt cggcaattga tccggtgcct agagaaggtg gcgcggggta
      301 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
      361 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
      421 caggaccggt tctagagcgc tgccaccatg gacaagaagt acagcatcgg cctggacatc
      481 ggcaccaact ctgtgggctg ggccgtgatc accgacgagt acaaggtgcc cagcaagaaa
      541 ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga agaacctgat cggagccctg
      601 ctgttcgaca gcggcgaaac agccgaggcc acccggctga agagaaccgc cagaagaaga
      661 tacaccagac ggaagaaccg gatctgctat ctgcaagaga tcttcagcaa cgagatggcc
      721 aaggtggacg acagcttctt ccacagactg gaaagagtcc ttcctggtgg aagaggataa
      781 gaagcacgag cggcacccca tcttcggcaa catcgtggac gaggtggcct accacgagaa
      841 gtaccccacc atctaccacc tgagaaagaa actggtggac agcaccgaca aggccgacct
      901 gcggctgatc tatctggccc tggcccacat gatcaagttc cggggccact tcctgatcgg
      961 agggcgacct gaaccccgac aacagcgacg tggaacagct gttcatccag ctgggtgcag
     1021 acctacaacc agctgtcgag gaaaacccca tcacgccagc ggggtgaccg ccaagggcat
     1081 cctgtcctgc cagactgacc agagcagacg gctggaaaat tcggatcgcc cagcctgccc
     1141 ggcgaaaaaa agaatggcct ggttccgaaa cctgattgcc ctggacctgg ccctggaacc
     1201 cactcaagag ccacttcgac ctgnnnnagg aatgcnanng cnnnganngn ncttacgacg
     1261 acgacctgaa aactgtggtn ccaaaattcg gggcaaannn at
//
