LOCUS       sequence-268005-        1264 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a non-targeting sgRNA and Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Hollstein PE, Eichner LJ, Brun SN, Kamireddy A, Svensson RU, Vera 
            LI, Ross DS, Rymoff TJ, Hutchins A, Galvez HM, Williams AE, 
            Shokhirev MN, Screaton RA, Berdeaux R, Shaw RJ
  TITLE     The AMPK-Related Kinases SIK1 and SIK3 Mediate Key Tumor-Suppressive
            Effects of LKB1 in NSCLC.
  JOURNAL   Cancer Discov. 2019 Nov;9(11):1606-1627. doi: 
            10.1158/2159-8290.CD-18-1261. Epub 2019 Jul 26.
  PUBMED    31350328
REFERENCE   2  (bases 1 to 1264)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-18-1261"; journalName: "Cancer Discov"; date: 
            "2019-11"; volume: "9"; issue: "11"; pages: "1606-1627"
COMMENT     Sequence Label: pLenti-Puro-sgNT
FEATURES             Location/Qualifiers
     source          1..1264
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        69..144
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        206..417
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      436..445
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gccgtaccga ttcttggctt tatatatctt gtggaaggac gaaacaccgc gaggtattcg
       61 gctccgcggt tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt
      121 gaaaaagtgg caccgagtcg gtgctttttt gaattcgcta gctaggtctt gaaaggagtg
      181 ggaattggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
      241 ttggggggag gggtcggcaa ttgatccggt gcctagagaa ggtggcgcgg ggtaaactgg
      301 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
      361 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggac
      421 cggttctaga gcgctgccac catggacaag aagtacagca tcggcctgga catcggcacc
      481 aactctgtgg gctgggccgt gatcaccgac gagtacaagg tgcccagcaa gaaattcaag
      541 gtgctgggca acaccgaccg gcacagcatc aagaagaacc tgatcggagc cctgctgttc
      601 gacagcggcg aaacagccga ggccacccgg ctgaagagaa ccgccagaag aagatacacc
      661 agacggaaga accggatctg ctatctgcaa gagatcttca gcaacgagat ggccaaggtg
      721 gacgacagct tcttccacag actggaagag tccttcctgg tggaagagga taagaagcac
      781 gagcggcacc ccatcttcgg caacatcgtg gacgaggtgg cctaccacga gaagtacccc
      841 accatctacc acctgagaaa gaaactggtg gacagcaccg acaagccgac ctgcggctga
      901 tctatctggc cctggcccac atgatcaagt tccggggcca cttcctgatc gagggcgacc
      961 tgaacccgac aacagcgacg tggacagctg ttcatccagc tggtgcagac tacaccagct
     1021 gttcgaggaa aaccccatca cgccagcgcg tgacgccaag ccatctgtct gcagactgac
     1081 agagcagacg ctgaaattct gatcgcccag ctgcccggcg agaagagatg ntgttcggaa
     1141 nctgatgcct gacctggntg acccactcag agcacttcga ctgngaagat gccantgcac
     1201 tgacaagaac ctagacgacg aactggncac ctgcgtncna gaattcggcg cacacaagta
     1261 tagc
//
