LOCUS       sequence-268009-        1235 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a human AMPKa2-targeting sgRNA and Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1235)
  AUTHORS   Hollstein PE, Eichner LJ, Brun SN, Kamireddy A, Svensson RU, Vera 
            LI, Ross DS, Rymoff TJ, Hutchins A, Galvez HM, Williams AE, 
            Shokhirev MN, Screaton RA, Berdeaux R, Shaw RJ
  TITLE     The AMPK-Related Kinases SIK1 and SIK3 Mediate Key Tumor-Suppressive
            Effects of LKB1 in NSCLC.
  JOURNAL   Cancer Discov. 2019 Nov;9(11):1606-1627. doi: 
            10.1158/2159-8290.CD-18-1261. Epub 2019 Jul 26.
  PUBMED    31350328
REFERENCE   2  (bases 1 to 1235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-18-1261"; journalName: "Cancer Discov"; date: 
            "2019-11"; volume: "9"; issue: "11"; pages: "1606-1627"
COMMENT     Sequence Label: pLenti-Puro-sgAMPKa2
FEATURES             Location/Qualifiers
     source          1..1235
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        70..145
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        207..418
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      437..446
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cgtatacgat tcttggcttt atatatcttg tggaaaggac gaaacaccga cgttatttaa
       61 gaagatccgg ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact
      121 tgaaaaagtg gcaccgagtc ggtgcttttt tgaattcgct agctaggtct tgaaaggagt
      181 gggaattggc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
      241 gttgggggga ggggtcggca attgatccgg tgcctagaga aggtggcgcg gggtaaactg
      301 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
      361 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagga
      421 ccggttctag agcgctgcca ccatggacaa gaagtacagc atcggcctgg acatcggcac
      481 caactctgtg ggctgggccg tgatcaccga cgagtacaag gtgcccagca agaaattcaa
      541 ggtgctgggc aacaccgacc ggcacagcat caagaagaac ctgatcggag ccctgctgtt
      601 cgacagcggc gaaacagccg aggccacccg gctgaagaga accgccagaa gaagatacac
      661 cagacggaag aaccggatct gctatctgca agagatcttc agcaacgaga tggccaaggt
      721 ggacgacagc ttcttccaca gactggaaga gtccttcctg gtggaagagg ataagaagca
      781 cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg gcctaccacg agaagtaccc
      841 caccatctac cacctgagaa agaaactggt ggacagcacc gacaaggccg acctgcggct
      901 gatctatctg gccctggccc acatgatcaa gttccggggc cacttcctga tcgagggcga
      961 cctgaacccc gacaacagcg acgtggacag ctgttcatcc agctggtgca gacctacacc
     1021 agctgttcga ggaaacccca tcacgccagc ggcgtgacgc caaggccatc ctgtctgcag
     1081 actgagcaga gcagacgctg gaaatctgat cgcccagctg cccggcgaga gagatgcctg
     1141 ttcggaactg atgccctgag ctggntgacc cactcagagc acttcgactn nngagatgcn
     1201 actgcgagcn ngaacaagga nncccttacg acnna
//
