LOCUS       sequence-268015-        1231 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a human MARK4-targeting sgRNA and Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Hollstein PE, Eichner LJ, Brun SN, Kamireddy A, Svensson RU, Vera 
            LI, Ross DS, Rymoff TJ, Hutchins A, Galvez HM, Williams AE, 
            Shokhirev MN, Screaton RA, Berdeaux R, Shaw RJ
  TITLE     The AMPK-Related Kinases SIK1 and SIK3 Mediate Key Tumor-Suppressive
            Effects of LKB1 in NSCLC.
  JOURNAL   Cancer Discov. 2019 Nov;9(11):1606-1627. doi: 
            10.1158/2159-8290.CD-18-1261. Epub 2019 Jul 26.
  PUBMED    31350328
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-18-1261"; journalName: "Cancer Discov"; date: 
            "2019-11"; volume: "9"; issue: "11"; pages: "1606-1627"
COMMENT     Sequence Label: pLenti-Puro-sgMARK4
FEATURES             Location/Qualifiers
     source          1..1231
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        71..146
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        208..419
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      438..447
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cccgtagtcg attcttggct ttatatatct tgtggaagga cgaaacaccg ttgaggttgt
       61 gcccgtcgaa gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac
      121 ttgaaaaagt ggcaccgagt cggtgctttt ttgaattcgc tagctaggtc ttgaaaggag
      181 tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
      241 agttgggggg aggggtcggc aattgatccg gtgcctagag aaggtggcgc ggggtaaact
      301 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
      361 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg
      421 accggttcta gagcgctgcc accatggaca agaagtacag catcggcctg gacatcggca
      481 ccaactctgt gggctgggcc gtgatcaccg acgagtacaa ggtgcccagc aagaaattca
      541 aggtgctggg caacaccgac cggcacagca tcaagaagaa cctgatcgga gccctgctgt
      601 tcgacagcgg cgaaacagcc gaggccaccc ggctgaagag aaccgccaga agaagataca
      661 ccagacggaa gaaccggatc tgctatctgc aagagatctt cagcaacgag atggccaagg
      721 tggacgacag cttcttccac agactggaag agtccttcct ggtggaagag gataagaagc
      781 acgagcggca ccccatcttc ggcaacatcg tggacgaggt ggcctaccac gagaagtacc
      841 ccaccatcta ccacctgaga aagaaactgg tggacagcac cgacaaggcc gacctgcggc
      901 tgatctatct ggccctggcc cacatgatca agttccgggc cacttcctga tcgagggcga
      961 cctgaacccc gacaacagcg acgtggacag ctgttcatcc agctggtgca gacctacacc
     1021 agctgttcga gaaacccatc acgcagcgcg tgacgcagca tctgtctgca gactgagcag
     1081 agcagacgct gaaatctgat cgccagctgc ccggcgagaa gagattgnct gtccgganct
     1141 gatgccctga gcctgcntga ccccactcag agcanncgan nncngatgcc aactgcactg
     1201 accagnncct cagaacgacg actggaacaa c
//
