LOCUS       sequence-268016-        1169 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a human NUAK1-targeting sgRNA and Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1169)
  AUTHORS   Hollstein PE, Eichner LJ, Brun SN, Kamireddy A, Svensson RU, Vera 
            LI, Ross DS, Rymoff TJ, Hutchins A, Galvez HM, Williams AE, 
            Shokhirev MN, Screaton RA, Berdeaux R, Shaw RJ
  TITLE     The AMPK-Related Kinases SIK1 and SIK3 Mediate Key Tumor-Suppressive
            Effects of LKB1 in NSCLC.
  JOURNAL   Cancer Discov. 2019 Nov;9(11):1606-1627. doi: 
            10.1158/2159-8290.CD-18-1261. Epub 2019 Jul 26.
  PUBMED    31350328
REFERENCE   2  (bases 1 to 1169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-18-1261"; journalName: "Cancer Discov"; date: 
            "2019-11"; volume: "9"; issue: "11"; pages: "1606-1627"
COMMENT     Sequence Label: pLenti-Puro-sgNUAK1
FEATURES             Location/Qualifiers
     source          1..1169
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        73..148
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        210..421
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      440..449
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cccttattcg atttcttggc tttatatatc ttgtggaaag gacgaaacac cgtgctataa
       61 aatccattcg tagttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca
      121 acttgaaaaa gtggcaccga gtcggtgctt ttttgaattc gctagctagg tcttgaaagg
      181 agtgggaatt ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
      241 gaagttgggg ggaggggtcg gcaattgatc cggtgcctag agaaggtggc gcggggtaaa
      301 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
      361 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
      421 ggaccggttc tagagcgctg ccaccatgga caagaagtac agcatcggcc tggacatcgg
      481 caccaactct gtgggctggg ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt
      541 caaggtgctg ggcaacaccg accggcacag catcaagaag aacctgatcg gagccctgct
      601 gttcgacagc ggcgaaacag ccgaggccac ccggctgaag agaaccgcca gaagaagata
      661 caccagacgg aagaaccgga tctgctatct gcaagagatc ttcagcaacg agatggccaa
      721 ggtggacgac agcttcttcc acagactgga agagtccttc ctggtggaag aggataagaa
      781 gcacgagcgg caccccatct tcggcaacat cgtggacgag gtggcctacc acgagaagta
      841 ccccaccatc taccacctga gaaagaaact ggtggacagc accgacaagg ccgacctgcg
      901 gctgatctat ctggccctgg cccacatgat caagttccgg ggccacttcc tgatcgaggg
      961 cgacctgaac cccgacaaca gcgacgtgga caagctgttc atccagctgg tgcagaccta
     1021 caaccagctg ttcgaggaaa ccccatcaac gccagcgcgt gacgccaggc atctgtctgc
     1081 agactgagca gagcagacgc tggaaatctg atcgcccagc tgcccgcgag aagaagaatg
     1141 nctgttcgga acctgattgc ctgagcctt
//
