LOCUS       sequence-268017-        1167 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a human NUAK2-targeting sgRNA and Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Hollstein PE, Eichner LJ, Brun SN, Kamireddy A, Svensson RU, Vera 
            LI, Ross DS, Rymoff TJ, Hutchins A, Galvez HM, Williams AE, 
            Shokhirev MN, Screaton RA, Berdeaux R, Shaw RJ
  TITLE     The AMPK-Related Kinases SIK1 and SIK3 Mediate Key Tumor-Suppressive
            Effects of LKB1 in NSCLC.
  JOURNAL   Cancer Discov. 2019 Nov;9(11):1606-1627. doi: 
            10.1158/2159-8290.CD-18-1261. Epub 2019 Jul 26.
  PUBMED    31350328
REFERENCE   2  (bases 1 to 1167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-18-1261"; journalName: "Cancer Discov"; date: 
            "2019-11"; volume: "9"; issue: "11"; pages: "1606-1627"
COMMENT     Sequence Label: pLenti-Puro-sgNUAK2
FEATURES             Location/Qualifiers
     source          1..1167
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        72..147
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        209..420
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      439..448
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cccgaattcg atttcttggc tttatatatc ttgtggaagg acgaaacacc gaagatctga
       61 tgcacatacg ggttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa
      121 cttgaaaaag tggcaccgag tcggtgcttt tttgaattcg ctagctaggt cttgaaagga
      181 gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
      241 aagttggggg gaggggtcgg caattgatcc ggtgcctaga gaaggtggcg cggggtaaac
      301 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
      361 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
      421 gaccggttct agagcgctgc caccatggac aagaagtaca gcatcggcct ggacatcggc
      481 accaactctg tgggctgggc cgtgatcacc gacgagtaca aggtgcccag caagaaattc
      541 aaggtgctgg gcaacaccga ccggcacagc atcaagaaga acctgatcgg agccctgctg
      601 ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga gaaccgccag aagaagatac
      661 accagacgga agaaccggat ctgctatctg caagagatct tcagcaacga gatggccaag
      721 gtggacgaca gcttcttcca cagactggaa gagtccttcc tggtggaaga ggataagaag
      781 cacgagcggc accccatctt cggcaacatc gtggacgagg tggcctacca cgagaagtac
      841 cccaccatct accacctgag aaagaaactg gtggacagca ccgacaagcc gacctgcggc
      901 tgatctatct ggccctggcc cacatgatca agttccgggg ccacttcctg atcgagggcg
      961 acctgaaccc cgacacagcg acgtggacag ctgttcatcc agctggtgca gactacacag
     1021 ctgttcgagg aacccatcac gcagcggcgt gacgcagcat ctgtctgcag actgagcaga
     1081 gcagacgctg aaatctgatc ggcagctgcc ggcagaagag atgnctgtcg gaactgatgc
     1141 ctgagctggg ctgacccact tcagagc
//
