LOCUS       sequence-268025-        1149 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a human CRTC2-targeting sgRNA and Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Hollstein PE, Eichner LJ, Brun SN, Kamireddy A, Svensson RU, Vera 
            LI, Ross DS, Rymoff TJ, Hutchins A, Galvez HM, Williams AE, 
            Shokhirev MN, Screaton RA, Berdeaux R, Shaw RJ
  TITLE     The AMPK-Related Kinases SIK1 and SIK3 Mediate Key Tumor-Suppressive
            Effects of LKB1 in NSCLC.
  JOURNAL   Cancer Discov. 2019 Nov;9(11):1606-1627. doi: 
            10.1158/2159-8290.CD-18-1261. Epub 2019 Jul 26.
  PUBMED    31350328
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-18-1261"; journalName: "Cancer Discov"; date: 
            "2019-11"; volume: "9"; issue: "11"; pages: "1606-1627"
COMMENT     Sequence Label: pLenti-Blast-sgCRTC2-2
FEATURES             Location/Qualifiers
     source          1..1149
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        77..152
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        214..425
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      444..453
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cgccgtagtt tcggattttc ttggctttat atatcttgtg gaaggacgaa acaccgacag
       61 gcccaaaaac tgcgacgttt tagagctaga aatagcaagt taaaataagg ctagtccgtt
      121 atcaacttga aaaagtggca ccgagtcggt gcttttttga attcgctagc taggtcttga
      181 aaggagtggg aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc
      241 ccgagaagtt ggggggaggg gtcggcaatt gatccggtgc ctagagaagg tggcgcgggg
      301 taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac
      361 cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa
      421 cacaggaccg gttctagagc gctgccacca tggacaagaa gtacagcatc ggcctggaca
      481 tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga gtacaaggtg cccagcaaga
      541 aattcaaggt gctgggcaac accgaccggc acagcatcaa gaagaacctg atcggagccc
      601 tgctgttcga cagcggcgaa acagccgagg ccacccggct gaagagaacc gccagaagaa
      661 gatacaccag acggaagaac cggatctgct atctgcaaga gatcttcagc aacgagatgg
      721 ccaaggtgga cgacagcttc ttccacagac tggaagagtc cttcctggtg gaagaggata
      781 agaagcacga gcggcacccc atcttcggca acatcgtgga cgaggtggcc taccacgaga
      841 agtaccccac catctaccac ctgagaaaga aactggtgga cagcaccgac aagccgacct
      901 gcggctgatc tatctggccc tggcccacat gatcaagttc cgggccactt cctgatcgag
      961 ggcgacctga accccgacaa cagcgacgtg gacaagctgt tcatccagct ggtgcagacc
     1021 tacaaccagc tgttcgagga aaccccatca cgccagcggc gtgacgctaa gccatctgtc
     1081 tgcagactga gcagagcaga cgctgaaaat ctgatcgccc tagctgcccg cgagaaaaaa
     1141 atnnctgnc
//
