LOCUS       sequence-268031-       15584 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For genome editing in plant by SpCas9 variant(SpCas9-NG) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15584)
  AUTHORS   Zhong Z, Sretenovic S, Ren Q, Yang L, Bao Y, Qi C, Yuan M, He Y, Liu
            S, Liu X, Wang J, Huang L, Wang Y, Baby D, Wang D, Zhang T, Qi Y, 
            Zhang Y
  TITLE     Improving Plant Genome Editing with High-Fidelity xCas9 and 
            Non-canonical PAM-Targeting Cas9-NG.
  JOURNAL   Mol Plant. 2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 
            10.1016/j.molp.2019.03.011.
  PUBMED    30928637
REFERENCE   2  (bases 1 to 15584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant. 
            2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 
            10.1016/j.molp.2019.03.011."
COMMENT     Sequence Label: pGEL063
FEATURES             Location/Qualifiers
     source          1..15584
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    103..277
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(1373..1718)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(1410..1430)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        1798..3794
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             3819..5537
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
     CDS             7935..7955
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7968..7988
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8001..8021
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      8032..8280
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     CDS             complement(8542..8847)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(8644..8663)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     misc_RNA        9174..9249
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(9261..9285)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    9315..9339
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             10640..11269
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             11698..12771
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      12837..13031
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(13267..13289)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    13365..13505
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     13420..13439
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(13520..13537)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13691..14279)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13771..13790)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(14366..15160)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcgg gggatctgga ttttagtact
      121 ggattttggt tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac
      181 atactaaggg tttcttatat gctcaacaca tgagcgaaac cctataggaa ccctaattcc
      241 cttatctggg aactactcac acattattat ggagaaaata gagcttgtcg atcgttaatt
      301 aatcacctcc aagatcccgg acgagtgctg gggcgtcggt ttccactatc ggcgagtact
      361 tctacacagc catcggtcca gacggccgcg cttctgcggg cgatttgtgt acgcccgaca
      421 gtcccggctc cggatcggac gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg
      481 aaattgccgt caaccaagct ctgatagagt tggtcaagac caatgcggag catatacgcc
      541 cggagccgcg gcgatcctgc aagctccgga tgcctccgct cgaagtagcg cgtctgctgc
      601 tccatacaag ccaaccacgg cctccagaag aagatgttgg cgacctcgta ttgggaatcc
      661 ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca
      721 ttgttggagc cgaaatccgc gtgcacgagg tgccggactt cggggcagtc ctcggcccaa
      781 agcatcagct catcgagagc ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt
      841 tgccagtgat acacatgggg atcagcaatc gcgcatatga aatcacgcca tgtagtgtat
      901 tgaccgattc cttgcggtcc gaatgggccg aacccgctcg tctggctaag atcggccgca
      961 gcgatcgcat ccatggcctc cgcgaccggc tgcagaacag cgggcagttc ggtttcaggc
     1021 aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc aataggtcag gctctcgctg
     1081 aattccccaa tgtcaagcac ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa
     1141 acataacgat ctttgtagaa accatcggcg cagctattta cccgcaggac atatccacgc
     1201 cctcctacat cgaagctgaa agcacgagat tcttcgccct ccgagagctg catcaggtcg
     1261 gacacgctgt cgaacttttc gatcagaaac ttctcgacag acgtcgcggt gagttcaggc
     1321 tttttcatcc taggagagat agatttgtag agagagactg gtgatttcag cgtgtcctct
     1381 ccaaatgaaa tgaacttcct tatatagagg aagggtcttg cgaaggatag tgggattgtg
     1441 cgtcatccct tacgtcagtg gagatatcac atcaatccac ttgctttgaa gacgtggttg
     1501 gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt
     1561 cggcagaggc atcttgaacg atagcctttc ctttatcgca atgatggcat ttgtaggtgc
     1621 caccttcctt ttctactgtc cttttgatga agtgacagat agctgggcaa tggaatccga
     1681 ggaggtttcc cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga
     1741 ctgtatcttt gatattcttg gagtagacga gagtgtcgtg ctccaccatg ggcgcgcctg
     1801 cagtgcagcg tgacccggtc gtgcccctct cttgagataa ggtgagcatt gcatgtctaa
     1861 gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca gtttatctat
     1921 ctttatacat atatttaaac tttactctac gaataatata atctatagta ctacaataat
     1981 atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag gacaattgag
     2041 tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt ctcctttttt
     2101 tttgcaaata gcttcaccta tataatactt catccatttt attagtacat ccatttaggg
     2161 tttagggtta atggttttta tagactaatt tttttagtac atctatttta ttctatttta
     2221 gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata atttagatat
     2281 aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga aattaaaaaa
     2341 actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa cgccgtcgac
     2401 gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag cgaagcagac
     2461 ggcacggcat ctctgtcgct gcctctggac ccctctcgag agttccgctc caccgttgga
     2521 cttgctccgc tgtcggcatc cagaaattgc gtggcggagc ggcagacgtg agccggcacg
     2581 gcaggcggcc tcctcctcct ctcacggcac cggcagctac gggggattcc tttcccaccg
     2641 ctccttcgct ttcccttcct cgcccgccgt aataaataga caccccctcc acaccctctt
     2701 tccccaacct cgtgttgttc ggagcgcaca cacacacaac cagaactccc ccaaatccac
     2761 ccgtcggcac ctccgcttca aggtacgccg ctcgtcctcc ccccccccct ctctaccttc
     2821 tcaagatcgg cgttccggtc catggttagg gcccggtagt tctacttctg ttcatgtttg
     2881 tgttagatcc gtgtttgtgt tagatccgtg ctactagcgt tcgtacacgg atgcgacctg
     2941 tacgtcagac acgttctgat tgctaacttg ccagtgtttc tctttgggga atcctgggat
     3001 ggctctagcc gttccgcaga cgggatcgat ttcatgattt tttttgtttc gttgcatagg
     3061 gtttggtttg cccttttcct ttatttcaat atatgccgtg cacttgtttg tcgggtcatc
     3121 ttttcatgct tttttttgtc ttggttgtga tgatgtggtc tggttgggcg gtcgttcaag
     3181 atcggagtag aattaattct gtttcaaact acctggtgga tttattaatt ttggatctgt
     3241 atgtgtgtgc catacatatt catagttacg aattgaagat gatggatgga aatatcgatc
     3301 taggataggt atacatgttg atgcgggttt tactgatgca tatacagaga tgctttttgt
     3361 tcgcttggtt gtgatgatgt ggtgtggttg ggcggtcgtt cattcgttca agatcggagt
     3421 agaatactgt ttcaaactac ctggtgtatt tattaatttt ggaactgtat gtgtgtgtca
     3481 tacatcttca tagttacgag tttaagatgg atggaaatat cgatctagga taggtataca
     3541 tgttgatgtg ggttttactg atgcatatac atgatggcat atgcagcatc tattcatatg
     3601 ctctaacctt gagtacctat ctattataat aaacaagtat gttttataat tattttgatc
     3661 ttgatatact tggatgatgg catatgcagc agctatatgt ggattttttt agccctgcct
     3721 tcatacgcta tttatttgct tggtactgtt tcttttgtcg atgctcaccc tgttgtttgg
     3781 tgttacttct gcagcctgca ggtagatcgc tcgtcgacat ggataagaag tactctatcg
     3841 gactcgatat cggaactaac tctgtgggat gggctgtgat caccgatgag tacaaggtgc
     3901 catctaagaa gttcaaggtt ctcggaaaca ccgataggca ctctatcaag aaaaacctta
     3961 tcggtgctct cctcttcgat tctggtgaaa ctgctgaggc taccagactc aagagaaccg
     4021 ctagaagaag gtacaccaga agaaagaaca ggatctgcta cctccaagag atcttctcta
     4081 acgagatggc taaagtggat gattcattct tccacaggct cgaagagtca ttcctcgtgg
     4141 aagaagataa gaagcacgag aggcacccta tcttcggaaa catcgttgat gaggtggcat
     4201 accacgagaa gtaccctact atctaccacc tcagaaagaa gctcgttgat tctactgata
     4261 aggctgatct caggctcatc tacctcgctc tcgctcacat gatcaagttc agaggacact
     4321 tcctcatcga gggtgatctc aaccctgata actctgatgt ggataagttg ttcatccagc
     4381 tcgtgcagac ctacaaccag cttttcgaag agaaccctat caacgcttca ggtgtggatg
     4441 ctaaggctat cctctctgct aggctctcta agtcaagaag gcttgagaac ctcattgctc
     4501 agctccctgg tgagaagaag aacggacttt tcggaaactt gatcgctctc tctctcggac
     4561 tcacccctaa cttcaagtct aacttcgatc tcgctgagga tgcaaagctc cagctctcaa
     4621 aggataccta cgatgatgat ctcgataacc tcctcgctca gatcggagat cagtacgctg
     4681 atttgttcct cgctgctaag aacctctctg atgctatcct cctcagtgat atcctcagag
     4741 tgaacaccga gatcaccaag gctccactct cagcttctat gatcaagaga tacgatgagc
     4801 accaccagga tctcacactt ctcaaggctc ttgttagaca gcagctccca gagaagtaca
     4861 aagagatttt cttcgatcag tctaagaacg gatacgctgg ttacatcgat ggtggtgcat
     4921 ctcaagaaga gttctacaag ttcatcaagc ctatcctcga gaagatggat ggaaccgagg
     4981 aactcctcgt gaagctcaat agagaggatc ttctcagaaa gcagaggacc ttcgataacg
     5041 gatctatccc tcatcagatc cacctcggag agttgcacgc tatccttaga aggcaagagg
     5101 atttctaccc attcctcaag gataacaggg aaaagattga gaagattctc accttcagaa
     5161 tcccttacta cgtgggacct ctcgctagag gaaactcaag attcgcttgg atgaccagaa
     5221 agtctgagga aaccatcacc ccttggaact tcgaagaggt ggtggataag ggtgctagtg
     5281 ctcagtcttt catcgagagg atgaccaact tcgataagaa ccttccaaac gagaaggtgc
     5341 tccctaagca ctctttgctc tacgagtact tcaccgtgta caacgagttg accaaggtta
     5401 agtacgtgac cgagggaatg aggaagcctg cttttttgtc aggtgagcaa aagaaggcta
     5461 tcgttgatct cttgttcaag accaacagaa aggtgaccgt gaagcagctc aaagaggatt
     5521 acttcaagaa aatcgagtgc ttcgattcag ttgagatttc tggtgttgag gataggttca
     5581 acgcatctct cggaacctac cacgatctcc tcaagatcat taaggataag gatttcttgg
     5641 ataacgagga aaacgaggat atcttggagg atatcgttct taccctcacc ctctttgaag
     5701 atagagagat gattgaagaa aggctcaaga cctacgctca tctcttcgat gataaggtga
     5761 tgaagcagtt gaagagaaga agatacactg gttggggaag gctctcaaga aagctcatta
     5821 acggaatcag ggataagcag tctggaaaga caatccttga tttcctcaag tctgatggat
     5881 tcgctaacag aaacttcatg cagctcatcc acgatgattc tctcaccttt aaagaggata
     5941 tccagaaggc tcaggtttca ggacagggtg atagtctcca tgagcatatc gctaacctcg
     6001 ctggatctcc tgcaatcaag aagggaatcc tccagactgt gaaggttgtg gatgagttgg
     6061 tgaaggtgat gggaaggcat aagcctgaga acatcgtgat cgaaatggct agagagaacc
     6121 agaccactca gaagggacag aagaactcta gggaaaggat gaagaggatc gaggaaggta
     6181 tcaaagagct tggatctcag atcctcaaag agcaccctgt tgagaacact cagctccaga
     6241 atgagaagct ctacctctac tacctccaga acggaaggga tatgtatgtg gatcaagagt
     6301 tggatatcaa caggctctct gattacgatg ttgatcatat cgtgccacag tcattcttga
     6361 aggatgattc tatcgataac aaggtgctca ccaggtctga taagaacagg ggtaagagtg
     6421 ataacgtgcc aagtgaagag gttgtgaaga aaatgaagaa ctattggagg cagctcctca
     6481 acgctaagct catcactcag agaaagttcg ataacttgac taaggctgag aggggaggac
     6541 tctctgaatt ggataaggca ggattcatca agaggcagct tgtggaaacc aggcagatca
     6601 ctaagcacgt tgcacagatc ctcgattcta ggatgaacac caagtacgat gagaacgata
     6661 agttgatcag ggaagtgaag gttatcaccc tcaagtcaaa gctcgtgtct gatttcagaa
     6721 aggatttcca attctacaag gtgagggaaa tcaacaacta ccaccacgct cacgatgctt
     6781 accttaacgc tgttgttgga accgctctca tcaagaagta tcctaagctc gagtcagagt
     6841 tcgtgtacgg tgattacaag gtgtacgatg tgaggaagat gatcgctaag tctgagcaag
     6901 agatcggaaa ggctaccgct aagtatttct tctactctaa catcatgaat ttcttcaaga
     6961 ccgagattac cctcgctaac ggtgagatca gaaagaggcc actcatcgag acaaacggtg
     7021 aaacaggtga gatcgtgtgg gataagggaa gggatttcgc taccgttaga aaggtgctct
     7081 ctatgccaca ggtgaacatc gttaagaaaa ccgaggtgca gaccggtgga ttctctaaag
     7141 agtctatcag gcctaagagg aactctgata agctcattgc taggaagaag gattgggacc
     7201 ctaagaaata cggtggtttc gtgtctccta ccgtggctta ctctgttctc gttgtggcta
     7261 aggttgagaa gggaaagagt aagaagctca agtctgttaa ggaacttctc ggaatcacta
     7321 tcatggaaag gtcatctttc gagaagaacc caatcgattt cctcgaggct aagggataca
     7381 aagaggttaa gaaggatctc atcatcaagc tcccaaagta ctcactcttc gaactcgaga
     7441 acggtagaaa gaggatgctc gcttctgcta ggttccttca aaagggaaac gagcttgctc
     7501 tcccatctaa gtacgttaac tttctttacc tcgcttctca ctacgagaag ttgaagggat
     7561 ctccagaaga taacgagcag aagcaacttt tcgttgagca gcacaagcac tacttggatg
     7621 agatcatcga gcagatctct gagttctcta aaagggtgat cctcgctgat gcaaacctcg
     7681 ataaggtgtt gtctgcttac aacaagcaca gagataagcc tatcagggaa caggcagaga
     7741 acatcatcca tctcttcacc cttaccaacc tcggtgctcc tagggctttc aagtacttcg
     7801 atacaaccat cgataggaag gtgtacaggt ctaccaaaga agtgctcgat gctaccctca
     7861 tccatcagtc tatcactgga ctctacgaga ctaggatcga tctctcacag ctcggtggtg
     7921 attcaagggc tgatcctaag aagaagagga aggtttctag ggcagatcca aagaagaagc
     7981 ggaaggtgtc tagagctgat ccgaagaaga agcgcaaggt gtgactcgag atatgaagat
     8041 gaagatgaaa tatttggtgt gtcaaataaa aagcttgtgt gcttaagttt gtgttttttt
     8101 cttggcttgt tgtgttatga atttgtggct ttttctaata ttaaatgaat gtaagatcac
     8161 attataatga ataaacaaat gtttctataa tccattgtga atgttttgtt ggatctcttc
     8221 tgcagcatat aactactgta tgtgctatgg tatggactat ggaatatgat taaagataag
     8281 aggatccgcg gatcatgaac caacggcctg gctgtatttg gtggttgtgt agggagatgg
     8341 ggagaagaaa agcccgattc tcttcgctgt gatgggctgg atgcatgcgg gggagcggga
     8401 ggcccaagta cgtgcacggt gagcggccca cagggcgagt gtgagcgcga gaggcgggag
     8461 gaacagttta gtaccacatt gcccagctaa ctcgaacgcg accaacttat aaacccgcgc
     8521 gctgtcgctt gtgtggagac cttatattcc ccagaacatc aggttaatgg cgtttttgat
     8581 gtcattttcg cggtggctga gatcagccac ttcttccccg ataacggaaa ccggcacact
     8641 ggccatatcg gtggtcatca tgcgccagct ttcatccccg atatgcacca ccgggtaaag
     8701 ttcacgggag actttatctg acagcagacg tgcactggcc agggggatca ccatccgtcg
     8761 cccgggcgtg tcaataatat cactctgtac atccacaaac agacgataac ggctctctct
     8821 tttataggtg taaaccttaa actgcatttc accagcccct gttctcgtca gcaaaagagc
     8881 cgttcatttc aataaaccgg gcgacctcag ccatcccttc ctgattttcc gctttccagc
     8941 gttcggcacg cagacgacgg gcttcattct gcatggttgt gcttaccaga ccggagatat
     9001 tgacatcata tatgccttga gcaactgata gctgtcgctg tcaactgtca ctgtaatacg
     9061 ctgcttcata gcatacctct ttttgacata cttcgggtat acatatcagt atatattctt
     9121 ataccgcaaa aatcagcgcg caaatacgca tactgttatc tggcttggtc tcagttttag
     9181 agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg
     9241 agtcggtgct ttttttctag acccagcttt cttgtacaaa gttggcatta ctgcgagctc
     9301 aaactatcag tgtttgacag gatatattgg cgggtaaacc taagagaaaa gagcgtttat
     9361 tagaataatc ggatatttaa aagggcgtga aaaggtttat ccgttcgtcc atttgtatgt
     9421 gcatgccaac cacagggttc ccctcgggat caaagtactt tgatccaacc cctccgctgc
     9481 tatagtgcag tcggcttctg acgttcagtg cagccgtctt ctgaaaacga catgtcgcac
     9541 aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt cctggcgttt tcttgtcgcg
     9601 tgttttagtc gcataaagta gaatacttgc gactagaacc ggagacatta cgccatgaac
     9661 aagagcgccg ccgctggcct gctgggctat gcccgcgtca gcaccgacga ccaggacttg
     9721 accaaccaac gggccgaact gcacgcggcc ggctgcacca agctgttttc cgagaagatc
     9781 accggcacca ggcgcgaccg cccggagctg gccaggatgc ttgaccacct acgccctggc
     9841 gacgttgtga cagtgaccag gctagaccgc ctggcccgca gcacccgcga cctactggac
     9901 attgccgagc gcatccagga ggccggcgcg ggcctgcgta gcctggcaga gccgtgggcc
     9961 gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt tcgccggcat tgccgagttc
    10021 gagcgttccc taatcatcga ccgcacccgg agcgggcgcg aggccgccaa ggcccgaggc
    10081 gtgaagtttg gcccccgccc taccctcacc ccggcacaga tcgcgcacgc ccgcgagctg
    10141 atcgaccagg aaggccgcac cgtgaaagag gcggctgcac tgcttggcgt gcatcgctcg
    10201 accctgtacc gcgcacttga gcgcagcgag gaagtgacgc ccaccgaggc caggcggcgc
    10261 ggtgccttcc gtgaggacgc attgaccgag gccgacgccc tggcggccgc cgagaatgaa
    10321 cgccaagagg aacaagcatg aaaccgcacc aggacggcca ggacgaaccg tttttcatta
    10381 ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt gttcgagccg cccgcgcacg
    10441 gctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc tgatgccaag ctggcggcct
    10501 ggccggccag cttggccgct gaagaaaccg agcgccgccg tctaaaaagg tgatgtgtat
    10561 ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat gatgcgatga gtaaataaac
    10621 aaatacgcaa ggggaacgca tgaaggttat cgctgtactt aaccagaaag gcgggtcagg
    10681 caagacgacc atcgcaaccc atctagcccg cgccctgcaa ctcgccgggg ccgatgttct
    10741 gttagtcgat tccgatcccc agggcagtgc ccgcgattgg gcggccgtgc gggaagatca
    10801 accgctaacc gttgtcggca tcgaccgccc gacgattgac cgcgacgtga aggccatcgg
    10861 ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg gcggacttgg ctgtgtccgc
    10921 gatcaaggca gccgacttcg tgctgattcc ggtgcagcca agcccttacg acatatgggc
    10981 caccgccgac ctggtggagc tggttaagca gcgcattgag gtcacggatg gaaggctaca
    11041 agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc atcggcggtg aggttgccga
    11101 ggcgctggcc gggtacgagc tgcccattct tgagtcccgt atcacgcagc gcgtgagcta
    11161 cccaggcact gccgccgccg gcacaaccgt tcttgaatca gaacccgagg gcgacgctgc
    11221 ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa ctcatttgag ttaatgaggt
    11281 aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg gccgtccgag cgcacgcagc
    11341 agcaaggctg caacgttggc cagcctggca gacacgccag ccatgaagcg ggtcaacttt
    11401 cagttgccgg cggaggatca caccaagctg aagatgtacg cggtacgcca aggcaagacc
    11461 attaccgagc tgctatctga atacatcgcg cagctaccag agtaaatgag caaatgaata
    11521 aatgagtaga tgaattttag cggctaaagg aggcggcatg gaaaatcaag aacaaccagg
    11581 caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc ggttggccag gcgtaagcgg
    11641 ctgggttgcc tgccggccct gcaatggcac tggaaccccc aagcccgagg aatcggcgtg
    11701 agcggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg
    11761 gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc
    11821 ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca
    11881 gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt
    11941 ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc
    12001 cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg
    12061 cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta
    12121 ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac
    12181 aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc
    12241 gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac
    12301 gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt
    12361 gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc
    12421 gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg
    12481 ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc
    12541 ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa
    12601 cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg
    12661 tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta
    12721 gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag
    12781 cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggcct ctttcctgtg
    12841 gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg
    12901 aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa
    12961 aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg
    13021 gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt
    13081 cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc
    13141 tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg
    13201 ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg
    13261 tgaaaacctc tgacacatgc agctcccgga aacggtcaca gcttgtctgt aagcggatgc
    13321 cgggagcaga caagcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
    13381 tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac
    13441 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
    13501 tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
    13561 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
    13621 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
    13681 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
    13741 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
    13801 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
    13861 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
    13921 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
    13981 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
    14041 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
    14101 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
    14161 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
    14221 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
    14281 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
    14341 ggattttggt catgcattct aggtactaaa acaattcatc cagtaaaata taatatttta
    14401 ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca tactgttctt
    14461 ccccgatatc ctccctgatc gaccggacgc agaaggcaat gtcataccac ttgtccgccc
    14521 tgccgcttct cccaagatca ataaagccac ttactttgcc atctttcaca aagatgttgc
    14581 tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa
    14641 aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca
    14701 catcggccag atcgttattc agtaagtaat ccaattcggc taagcggctg tctaagctat
    14761 tcgtataggg acaatccgat atgtcgatgg agtgaaagag cctgatgcac tccgcataca
    14821 gctcgataat cttttcaggg ctttgttcat cttcatactc ttccgagcaa aggacgccat
    14881 cggcctcact catgagcaga ttgctccagc catcatgccg ttcaaagtgc aggacctttg
    14941 gaacaggcag ctttccttcc agccatagca tcatgtcctt ttcccgttcc acatcatagg
    15001 tggtcccttt ataccggctg tccgtcattt ttaaatatag gttttcattt tctcccacca
    15061 gcttatatac cttagcagga gacattcctt ccgtatcttt tacgcagcgg tatttttcga
    15121 tcagtttttt caattccggt gatattctca ttttagccat ttattatttc cttcctcttt
    15181 tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact ccaattcact
    15241 gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt tgttttcaaa
    15301 gttggcgtat aacatagtat cgacggagcc gattttgaaa ccgcggtgat cacaggcagc
    15361 aacgctctgt catcgttaca atcaacatgc taccctccgc gagatcatcc gtgtttcaaa
    15421 cccggcagct tagttgccgt tcttccgaat agcatcggta acatgagcaa agtctgccgc
    15481 cttacaacgg ctctcccgct gacgccgtcc cggactgatg ggctgcctgt atcgagtggt
    15541 gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctgg
//
