LOCUS       sequence-268034-       18230 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For genome editing in plant by SpCas9 variant(SpCas9-NG) 
            (D10A)-PmCDA1-UGI base editor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18230)
  AUTHORS   Zhong Z, Sretenovic S, Ren Q, Yang L, Bao Y, Qi C, Yuan M, He Y, Liu
            S, Liu X, Wang J, Huang L, Wang Y, Baby D, Wang D, Zhang T, Qi Y, 
            Zhang Y
  TITLE     Improving Plant Genome Editing with High-Fidelity xCas9 and 
            Non-canonical PAM-Targeting Cas9-NG.
  JOURNAL   Mol Plant. 2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 
            10.1016/j.molp.2019.03.011.
  PUBMED    30928637
REFERENCE   2  (bases 1 to 18230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Plant. 
            2019 Mar 27. pii: S1674-2052(19)30124-8. doi: 
            10.1016/j.molp.2019.03.011."
COMMENT     Sequence Label: pGEL066
FEATURES             Location/Qualifiers
     source          1..18230
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    103..277
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     promoter        complement(1373..1718)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(1410..1430)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        1798..3795
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             7936..7956
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8164..8229
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             8863..8883
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             8890..9141
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="MTNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHT
                     AYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             9142..9195
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             9382..9402
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9415..9435
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9448..9468
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      9479..9727
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     CDS             complement(9989..10294)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(10091..10110)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     misc_RNA        10621..10696
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(10708..10732)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    10762..10786
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             12087..12716
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             13145..14218
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      14284..14478
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(14714..14736)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    14812..14952
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     14867..14886
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(14967..14984)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(15138..15726)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(15218..15237)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(17012..17806)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcgg gggatctgga ttttagtact
      121 ggattttggt tttaggaatt agaaatttta ttgatagaag tattttacaa atacaaatac
      181 atactaaggg tttcttatat gctcaacaca tgagcgaaac cctataggaa ccctaattcc
      241 cttatctggg aactactcac acattattat ggagaaaata gagcttgtcg atcgttaatt
      301 aatcacctcc aagatcccgg acgagtgctg gggcgtcggt ttccactatc ggcgagtact
      361 tctacacagc catcggtcca gacggccgcg cttctgcggg cgatttgtgt acgcccgaca
      421 gtcccggctc cggatcggac gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg
      481 aaattgccgt caaccaagct ctgatagagt tggtcaagac caatgcggag catatacgcc
      541 cggagccgcg gcgatcctgc aagctccgga tgcctccgct cgaagtagcg cgtctgctgc
      601 tccatacaag ccaaccacgg cctccagaag aagatgttgg cgacctcgta ttgggaatcc
      661 ccgaacatcg cctcgctcca gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca
      721 ttgttggagc cgaaatccgc gtgcacgagg tgccggactt cggggcagtc ctcggcccaa
      781 agcatcagct catcgagagc ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt
      841 tgccagtgat acacatgggg atcagcaatc gcgcatatga aatcacgcca tgtagtgtat
      901 tgaccgattc cttgcggtcc gaatgggccg aacccgctcg tctggctaag atcggccgca
      961 gcgatcgcat ccatggcctc cgcgaccggc tgcagaacag cgggcagttc ggtttcaggc
     1021 aggtcttgca acgtgacacc ctgtgcacgg cgggagatgc aataggtcag gctctcgctg
     1081 aattccccaa tgtcaagcac ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa
     1141 acataacgat ctttgtagaa accatcggcg cagctattta cccgcaggac atatccacgc
     1201 cctcctacat cgaagctgaa agcacgagat tcttcgccct ccgagagctg catcaggtcg
     1261 gacacgctgt cgaacttttc gatcagaaac ttctcgacag acgtcgcggt gagttcaggc
     1321 tttttcatcc taggagagat agatttgtag agagagactg gtgatttcag cgtgtcctct
     1381 ccaaatgaaa tgaacttcct tatatagagg aagggtcttg cgaaggatag tgggattgtg
     1441 cgtcatccct tacgtcagtg gagatatcac atcaatccac ttgctttgaa gacgtggttg
     1501 gaacgtcttc tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt
     1561 cggcagaggc atcttgaacg atagcctttc ctttatcgca atgatggcat ttgtaggtgc
     1621 caccttcctt ttctactgtc cttttgatga agtgacagat agctgggcaa tggaatccga
     1681 ggaggtttcc cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga
     1741 ctgtatcttt gatattcttg gagtagacga gagtgtcgtg ctccaccatg ggcgcgcctg
     1801 cagtgcagcg tgacccggtc gtgcccctct cttgagataa ggtgagcatt gcatgtctaa
     1861 gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca gtttatctat
     1921 ctttatacat atatttaaac tttactctac gaataatata atctatagta ctacaataat
     1981 atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag gacaattgag
     2041 tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt ctcctttttt
     2101 tttgcaaata gcttcaccta tataatactt catccatttt attagtacat ccatttaggg
     2161 tttagggtta atggttttta tagactaatt tttttagtac atctatttta ttctatttta
     2221 gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata atttagatat
     2281 aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga aattaaaaaa
     2341 actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa cgccgtcgac
     2401 gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag cgaagcagac
     2461 ggcacggcat ctctgtcgct gcctctggac ccctctcgag agttccgctc caccgttgga
     2521 cttgctccgc tgtcggcatc cagaaattgc gtggcggagc ggcagacgtg agccggcacg
     2581 gcaggcggcc tcctcctcct ctcacggcac cggcagctac gggggattcc tttcccaccg
     2641 ctccttcgct ttcccttcct cgcccgccgt aataaataga caccccctcc acaccctctt
     2701 tccccaacct cgtgttgttc ggagcgcaca cacacacaac cagaactccc ccaaatccac
     2761 ccgtcggcac ctccgcttca aggtacgccg ctcgtcctcc cccccccccn tctctacctt
     2821 ctcaagatcg gcgttccggt ccatggttag ggcccggtag ttctacttct gttcatgttt
     2881 gtgttagatc cgtgtttgtg ttagatccgt gctactagcg ttcgtacacg gatgcgacct
     2941 gtacgtcaga cacgttctga ttgctaactt gccagtgttt ctctttgggg aatcctggga
     3001 tggctctagc cgttccgcag acgggatcga tttcatgatt ttttttgttt cgttgcatag
     3061 ggtttggttt gcccttttcc tttatttcaa tatatgccgt gcacttgttt gtcgggtcat
     3121 cttttcatgc ttttttttgt cttggttgtg atgatgtggt ctggttgggc ggtcgttcaa
     3181 gatcggagta gaattaattc tgtttcaaac tacctggtgg atttattaat tttggatctg
     3241 tatgtgtgtg ccatacatat tcatagttac gaattgaaga tgatggatgg aaatatcgat
     3301 ctaggatagg tatacatgtt gatgcgggtt ttactgatgc atatacagag atgctttttg
     3361 ttcgcttggt tgtgatgatg tggtgtggtt gggcggtcgt tcattcgttc aagatcggag
     3421 tagaatactg tttcaaacta cctggtgtat ttattaattt tggaactgta tgtgtgtgtc
     3481 atacatcttc atagttacga gtttaagatg gatggaaata tcgatctagg ataggtatac
     3541 atgttgatgt gggttttact gatgcatata catgatggca tatgcagcat ctattcatat
     3601 gctctaacct tgagtaccta tctattataa taaacaagta tgttttataa ttattttgat
     3661 cttgatatac ttggatgatg gcatatgcag cagctatatg tggatttttt tagccctgcc
     3721 ttcatacgct atttatttgc ttggtactgt ttcttttgtc gatgctcacc ctgttgtttg
     3781 gtgttacttc tgcagcctgc aggtagatcg ctcgtcgaca tggataagaa gtactctatc
     3841 ggactcgcca tcggaactaa ctctgtggga tgggctgtga tcaccgatga gtacaaggtg
     3901 ccatctaaga agttcaaggt tctcggaaac accgataggc actctatcaa gaaaaacctt
     3961 atcggtgctc tcctcttcga ttctggtgaa actgctgagg ctaccagact caagagaacc
     4021 gctagaagaa ggtacaccag aagaaagaac aggatctgct acctccaaga gatcttctct
     4081 aacgagatgg ctaaagtgga tgattcattc ttccacaggc tcgaagagtc attcctcgtg
     4141 gaagaagata agaagcacga gaggcaccct atcttcggaa acatcgttga tgaggtggca
     4201 taccacgaga agtaccctac tatctaccac ctcagaaaga agctcgttga ttctactgat
     4261 aaggctgatc tcaggctcat ctacctcgct ctcgctcaca tgatcaagtt cagaggacac
     4321 ttcctcatcg agggtgatct caaccctgat aactctgatg tggataagtt gttcatccag
     4381 ctcgtgcaga cctacaacca gcttttcgaa gagaacccta tcaacgcttc aggtgtggat
     4441 gctaaggcta tcctctctgc taggctctct aagtcaagaa ggcttgagaa cctcattgct
     4501 cagctccctg gtgagaagaa gaacggactt ttcggaaact tgatcgctct ctctctcgga
     4561 ctcaccccta acttcaagtc taacttcgat ctcgctgagg atgcaaagct ccagctctca
     4621 aaggatacct acgatgatga tctcgataac ctcctcgctc agatcggaga tcagtacgct
     4681 gatttgttcc tcgctgctaa gaacctctct gatgctatcc tcctcagtga tatcctcaga
     4741 gtgaacaccg agatcaccaa ggctccactc tcagcttcta tgatcaagag atacgatgag
     4801 caccaccagg atctcacact tctcaaggct cttgttagac agcagctccc agagaagtac
     4861 aaagagattt tcttcgatca gtctaagaac ggatacgctg gttacatcga tggtggtgca
     4921 tctcaagaag agttctacaa gttcatcaag cctatcctcg agaagatgga tggaaccgag
     4981 gaactcctcg tgaagctcaa tagagaggat cttctcagaa agcagaggac cttcgataac
     5041 ggatctatcc ctcatcagat ccacctcgga gagttgcacg ctatccttag aaggcaagag
     5101 gatttctacc cattcctcaa ggataacagg gaaaagattg agaagattct caccttcaga
     5161 atcccttact acgtgggacc tctcgctaga ggaaactcaa gattcgcttg gatgaccaga
     5221 aagtctgagg aaaccatcac cccttggaac ttcgaagagg tggtggataa gggtgctagt
     5281 gctcagtctt tcatcgagag gatgaccaac ttcgataaga accttccaaa cgagaaggtg
     5341 ctccctaagc actctttgct ctacgagtac ttcaccgtgt acaacgagtt gaccaaggtt
     5401 aagtacgtga ccgagggaat gaggaagcct gcttttttgt caggtgagca aaagaaggct
     5461 atcgttgatc tcttgttcaa gaccaacaga aaggtgaccg tgaagcagct caaagaggat
     5521 tacttcaaga aaatcgagtg cttcgattca gttgagattt ctggtgttga ggataggttc
     5581 aacgcatctc tcggaaccta ccacgatctc ctcaagatca ttaaggataa ggatttcttg
     5641 gataacgagg aaaacgagga tatcttggag gatatcgttc ttaccctcac cctctttgaa
     5701 gatagagaga tgattgaaga aaggctcaag acctacgctc atctcttcga tgataaggtg
     5761 atgaagcagt tgaagagaag aagatacact ggttggggaa ggctctcaag aaagctcatt
     5821 aacggaatca gggataagca gtctggaaag acaatccttg atttcctcaa gtctgatgga
     5881 ttcgctaaca gaaacttcat gcagctcatc cacgatgatt ctctcacctt taaagaggat
     5941 atccagaagg ctcaggtttc aggacagggt gatagtctcc atgagcatat cgctaacctc
     6001 gctggatctc ctgcaatcaa gaagggaatc ctccagactg tgaaggttgt ggatgagttg
     6061 gtgaaggtga tgggaaggca taagcctgag aacatcgtga tcgaaatggc tagagagaac
     6121 cagaccactc agaagggaca gaagaactct agggaaagga tgaagaggat cgaggaaggt
     6181 atcaaagagc ttggatctca gatcctcaaa gagcaccctg ttgagaacac tcagctccag
     6241 aatgagaagc tctacctcta ctacctccag aacggaaggg atatgtatgt ggatcaagag
     6301 ttggatatca acaggctctc tgattacgat gttgatcata tcgtgccaca gtcattcttg
     6361 aaggatgatt ctatcgataa caaggtgctc accaggtctg ataagaacag gggtaagagt
     6421 gataacgtgc caagtgaaga ggttgtgaag aaaatgaaga actattggag gcagctcctc
     6481 aacgctaagc tcatcactca gagaaagttc gataacttga ctaaggctga gaggggagga
     6541 ctctctgaat tggataaggc aggattcatc aagaggcagc ttgtggaaac caggcagatc
     6601 actaagcacg ttgcacagat cctcgattct aggatgaaca ccaagtacga tgagaacgat
     6661 aagttgatca gggaagtgaa ggttatcacc ctcaagtcaa agctcgtgtc tgatttcaga
     6721 aaggatttcc aattctacaa ggtgagggaa atcaacaact accaccacgc tcacgatgct
     6781 taccttaacg ctgttgttgg aaccgctctc atcaagaagt atcctaagct cgagtcagag
     6841 ttcgtgtacg gtgattacaa ggtgtacgat gtgaggaaga tgatcgctaa gtctgagcaa
     6901 gagatcggaa aggctaccgc taagtatttc ttctactcta acatcatgaa tttcttcaag
     6961 accgagatta ccctcgctaa cggtgagatc agaaagaggc cactcatcga gacaaacggt
     7021 gaaacaggtg agatcgtgtg ggataaggga agggatttcg ctaccgttag aaaggtgctc
     7081 tctatgccac aggtgaacat cgttaagaaa accgaggtgc agaccggtgg attctctaaa
     7141 gagtctatca ggcctaagag gaactctgat aagctcattg ctaggaagaa ggattgggac
     7201 cctaagaaat acggtggttt cgtgtctcct accgtggctt actctgttct cgttgtggct
     7261 aaggttgaga agggaaagag taagaagctc aagtctgtta aggaacttct cggaatcact
     7321 atcatggaaa ggtcatcttt cgagaagaac ccaatcgatt tcctcgaggc taagggatac
     7381 aaagaggtta agaaggatct catcatcaag ctcccaaagt actcactctt cgaactcgag
     7441 aacggtagaa agaggatgct cgcttctgct aggttccttc aaaagggaaa cgagcttgct
     7501 ctcccatcta agtacgttaa ctttctttac ctcgcttctc actacgagaa gttgaaggga
     7561 tctccagaag ataacgagca gaagcaactt ttcgttgagc agcacaagca ctacttggat
     7621 gagatcatcg agcagatctc tgagttctct aaaagggtga tcctcgctga tgcaaacctc
     7681 gataaggtgt tgtctgctta caacaagcac agagataagc ctatcaggga acaggcagag
     7741 aacatcatcc atctcttcac ccttaccaac ctcggtgctc ctagggcttt caagtacttc
     7801 gatacaacca tcgataggaa ggtgtacagg tctaccaaag aagtgctcga tgctaccctc
     7861 atccatcagt ctatcactgg actctacgag actaggatcg atctctcaca gctcggtggt
     7921 gattcaaggg ctgatcctaa gaagaagagg aaggttggag gtggcggtac aggagggggt
     7981 gggtccgctg agtatgtcag ggcgttgttc gacttcaatg gaaacgacga ggaagatctg
     8041 ccttttaaaa agggagatat tctcaggatc agagataagc cggaagaaca atggtggaac
     8101 gctgaagact ctgaaggtaa gagaggtatg attcttgtcc cctacgtcga gaagtattcg
     8161 ggtgactata aagaccacga tggagattat aaggaccacg atatagatta taaggatgat
     8221 gatgataaga gcggaatgac cgatgcagag tacgtcagga ttcatgagaa acttgacatc
     8281 tacacgttta agaaacagtt tttcaacaac aaaaaatctg ttagtcaccg ctgttacgtg
     8341 ctgttcgaat tgaaacgcag aggtgagagg agagcctgct tttggggcta tgccgtcaac
     8401 aagccgcaaa gcggcacaga aaggggcatt cacgcggaga tatttagcat tagaaaggtc
     8461 gaggaatacc ttcgggataa tcccgggcaa ttcactatca attggtactc ttcatggtcc
     8521 ccgtgtgcag attgcgctga aaagatactg gagtggtata atcaagaact cagaggaaac
     8581 ggtcacaccc tcaagatttg ggcttgcaag ctttactacg agaaaaatgc aaggaaccag
     8641 atcggcctct ggaacttgcg cgacaacggc gtggggttga atgtgatggt gtcggagcat
     8701 taccagtgct gccggaagat attcattcag tcgtcacata atcaattgaa cgagaatagg
     8761 tggctcgaaa aaaccctgaa gcgggccgag aagtggagga gtgaactctc gataatgatc
     8821 caggttaaaa tactgcatac taccaaatct ccggcggtgg gaccgaagaa gaagcgcaag
     8881 gtggggacca tgactaatct ctcagatata atcgagaagg aaacaggaaa gcaactggtc
     8941 atccaagaat cgattttgat gcttcccgaa gaagtcgaag aagttatagg aaataagccc
     9001 gagtctgaca tactggttca cacagcgtac gatgaaagta cggacgagaa tgtcatgttg
     9061 ctgacatcgg acgcacctga atacaagcct tgggctctgg tcatacaaga tagtaacgga
     9121 gaaaataaga ttaaaatgct tgaaggaagg ggctccctgc tgacatgtgg agatgtggaa
     9181 gagaatccag gaccatccag gatgatagag caagatggtt gtacccaagt gctgcggccc
     9241 ctcggatggc gggggtacag cgccatgact ggccataata gacagtccgc tgctctcatg
     9301 cctccatgca gcggatgtca gaggagggga gctaggttct tcctgtctag acctacctgc
     9361 cccgtgccct caagggctga tcctaagaag aagaggaagg tttctagggc agatccaaag
     9421 aagaagcgga aggtgtctag agctgatccg aagaagaagc gcaaggtgtg actcgagata
     9481 tgaagatgaa gatgaaatat ttggtgtgtc aaataaaaag cttgtgtgct taagtttgtg
     9541 tttttttctt ggcttgttgt gttatgaatt tgtggctttt tctaatatta aatgaatgta
     9601 agatcacatt ataatgaata aacaaatgtt tctataatcc attgtgaatg ttttgttgga
     9661 tctcttctgc agcatataac tactgtatgt gctatggtat ggactatgga atatgattaa
     9721 agataagagg atccgcggat catgaaccaa cggcctggct gtatttggtg gttgtgtagg
     9781 gagatgggga gaagaaaagc ccgattctct tcgctgtgat gggctggatg catgcggggg
     9841 agcgggaggc ccaagtacgt gcacggtgag cggcccacag ggcgagtgtg agcgcgagag
     9901 gcgggaggaa cagtttagta ccacattgcc cagctaactc gaacgcgacc aacttataaa
     9961 cccgcgcgct gtcgcttgtg tggagacctt atattcccca gaacatcagg ttaatggcgt
    10021 ttttgatgtc attttcgcgg tggctgagat cagccacttc ttccccgata acggaaaccg
    10081 gcacactggc catatcggtg gtcatcatgc gccagctttc atccccgata tgcaccaccg
    10141 ggtaaagttc acgggagact ttatctgaca gcagacgtgc actggccagg gggatcacca
    10201 tccgtcgccc gggcgtgtca ataatatcac tctgtacatc cacaaacaga cgataacggc
    10261 tctctctttt ataggtgtaa accttaaact gcatttcacc agcccctgtt ctcgtcagca
    10321 aaagagccgt tcatttcaat aaaccgggcg acctcagcca tcccttcctg attttccgct
    10381 ttccagcgtt cggcacgcag acgacgggct tcattctgca tggttgtgct taccagaccg
    10441 gagatattga catcatatat gccttgagca actgatagct gtcgctgtca actgtcactg
    10501 taatacgctg cttcatagca tacctctttt tgacatactt cgggtataca tatcagtata
    10561 tattcttata ccgcaaaaat cagcgcgcaa atacgcatac tgttatctgg cttggtctca
    10621 gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt
    10681 ggcaccgagt cggtgctttt tttctagacc cagctttctt gtacaaagtt ggcattactg
    10741 cgagctcaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa gagaaaagag
    10801 cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg ttcgtccatt
    10861 tgtatgtgca tgccaaccac agggttcccc tcgggatcaa agtactttga tccaacccct
    10921 ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg aaaacgacat
    10981 gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg cccttttcct ggcgttttct
    11041 tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac tagaaccgga gacattacgc
    11101 catgaacaag agcgccgccg ctggcctgct gggctatgcc cgcgtcagca ccgacgacca
    11161 ggacttgacc aaccaacggg ccgaactgca cgcggccggc tgcaccaagc tgttttccga
    11221 gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc aggatgcttg accacctacg
    11281 ccctggcgac gttgtgacag tgaccaggct agaccgcctg gcccgcagca cccgcgacct
    11341 actggacatt gccgagcgca tccaggaggc cggcgcgggc ctgcgtagcc tggcagagcc
    11401 gtgggccgac accaccacgc cggccggccg catggtgttg accgtgttcg ccggcattgc
    11461 cgagttcgag cgttccctaa tcatcgaccg cacccggagc gggcgcgagg ccgccaaggc
    11521 ccgaggcgtg aagtttggcc cccgccctac cctcaccccg gcacagatcg cgcacgcccg
    11581 cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg gctgcactgc ttggcgtgca
    11641 tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa gtgacgccca ccgaggccag
    11701 gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc gacgccctgg cggccgccga
    11761 gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg acggccagga cgaaccgttt
    11821 ttcattaccg aagagatcga ggcggagatg atcgcggccg ggtacgtgtt cgagccgccc
    11881 gcgcacggct caaccgtgcg gctgcatgaa atcctggccg gtttgtctga tgccaagctg
    11941 gcggcctggc cggccagctt ggccgctgaa gaaaccgagc gccgccgtct aaaaaggtga
    12001 tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg cgtatatgat gcgatgagta
    12061 aataaacaaa tacgcaaggg gaacgcatga aggttatcgc tgtacttaac cagaaaggcg
    12121 ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc cctgcaactc gccggggccg
    12181 atgttctgtt agtcgattcc gatccccagg gcagtgcccg cgattgggcg gccgtgcggg
    12241 aagatcaacc gctaaccgtt gtcggcatcg accgcccgac gattgaccgc gacgtgaagg
    12301 ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc ccaggcggcg gacttggctg
    12361 tgtccgcgat caaggcagcc gacttcgtgc tgattccggt gcagccaagc ccttacgaca
    12421 tatgggccac cgccgacctg gtggagctgg ttaagcagcg cattgaggtc acggatggaa
    12481 ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg cacgcgcatc ggcggtgagg
    12541 ttgccgaggc gctggccggg tacgagctgc ccattcttga gtcccgtatc acgcagcgcg
    12601 tgagctaccc aggcactgcc gccgccggca caaccgttct tgaatcagaa cccgagggcg
    12661 acgctgcccg cgaggtccag gcgctggccg ctgaaattaa atcaaaactc atttgagtta
    12721 atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta agtgccggcc gtccgagcgc
    12781 acgcagcagc aaggctgcaa cgttggccag cctggcagac acgccagcca tgaagcgggt
    12841 caactttcag ttgccggcgg aggatcacac caagctgaag atgtacgcgg tacgccaagg
    12901 caagaccatt accgagctgc tatctgaata catcgcgcag ctaccagagt aaatgagcaa
    12961 atgaataaat gagtagatga attttagcgg ctaaaggagg cggcatggaa aatcaagaac
    13021 aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg aacgggcggt tggccaggcg
    13081 taagcggctg ggttgcctgc cggccctgca atggcactgg aacccccaag cccgaggaat
    13141 cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc ggcgcggcgc tgggtgatga
    13201 cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg caacgcatcg aggcagaagc
    13261 acgccccggt gaatcgtggc aagcggccgc tgatcgaatc cgcaaagaat cccggcaacc
    13321 gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag ggcgacgagc aaccagattt
    13381 tttcgttccg atgctctatg acgtgggcac ccgcgatagt cgcagcatca tggacgtggc
    13441 cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag gtgatccgct acgagcttcc
    13501 agacgggcac gtagaggttt ccgcagggcc ggccggcatg gccagtgtgt gggattacga
    13561 cctggtactg atggcggttt cccatctaac cgaatccatg aaccgatacc gggaagggaa
    13621 gggagacaag cccggccgcg tgttccgtcc acacgttgcg gacgtactca agttctgccg
    13681 gcgagccgat ggcggaaagc agaaagacga cctggtagaa acctgcattc ggttaaacac
    13741 cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac ggccgcctgg tgacggtatc
    13801 cgagggtgaa gccttgatta gccgctacaa gatcgtaaag agcgaaaccg ggcggccgga
    13861 gtacatcgag atcgagctag ctgattggat gtaccgcgag atcacagaag gcaagaaccc
    13921 ggacgtgctg acggttcacc ccgattactt tttgatcgat cccggcatcg gccgttttct
    13981 ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc agatggttgt tcaagacgat
    14041 ctacgaacgc agtggcagcg ccggagagtt caagaagttc tgtttcaccg tgcgcaagct
    14101 gatcgggtca aatgacctgc cggagtacga tttgaaggag gaggcggggc aggctggccc
    14161 gatcctagtc atgcgctacc gcaacctgat cgagggcgaa gcatccgccg gttcctaatg
    14221 tacggagcag atgctagggc aaattgccct agcaggggaa aaaggtcgaa aaggcctctt
    14281 tcctgtggat agcacgtaca ttgggaaccc aaagccgtac attgggaacc ggaacccgta
    14341 cattgggaac ccaaagccgt acattgggaa ccggtcacac atgtaagtga ctgatataaa
    14401 agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa cttattaaaa ctcttaaaac
    14461 ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc gaagagctgc aaaaagcgcc
    14521 tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg cgtcggccta tcgcggccgc
    14581 tggccgctca aaaatggctg gcctacggcc aggcaatcta ccagggcgcg gacaagccgc
    14641 gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc ctgcctcgcg cgtttcggtg
    14701 atgacggtga aaacctctga cacatgcagc tcccggaaac ggtcacagct tgtctgtaag
    14761 cggatgccgg gagcagacaa gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
    14821 gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag
    14881 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
    14941 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
    15001 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
    15061 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
    15121 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
    15181 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
    15241 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
    15301 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
    15361 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
    15421 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
    15481 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
    15541 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
    15601 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
    15661 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    15721 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
    15781 cgttaaggga ttttggtcat gcattctagg gaaggtgcga acaagtccct gatatgagat
    15841 catgtttgtc atctggagcc atagaacagg gttcatcatg agtcatcaac ttaccttcgc
    15901 cgacagtgaa ttcagcagta agcgccgtca gaccagaaaa gagattttct tgtcccgcat
    15961 ggagcagatt ctgccatggc aaaacatggt ggaagtcatc gagccgtttt accccaaggc
    16021 tggtaatggc cggcgacctt atccgctgga aaccatgcta cgcattcact gcatgcagca
    16081 ttggtacaac ctgagcgatg gcgcgatgga agatgctctg tacgaaatcg cctccatgcg
    16141 tctgtttgcc cggttatccc tggatagcgc cttgccggac cgcaccacca tcatgaattt
    16201 ccgccacctg ctggagcagc atcaactggc ccgccaattg ttcaagacca tcaatcgctg
    16261 gctggccgaa gcaggcgtca tgatgactca aggcaccttg gtcgatgcca ccatcattga
    16321 ggcacccagc tcgaccaaga acaaagagca gcaacgcgat ccggagatgc atcagaccaa
    16381 gaaaggcaat cagtggcact ttggcatgaa ggcccacatt ggtgtcgatg ccaagagtgg
    16441 cctgacccac agcctggtca ccaccgcggc caacgagcat gacctcaatc agctgggtaa
    16501 tctgctgcat ggagaggagc aatttgtctc agccgatgcc ggctaccaag gggcgccaca
    16561 gcgcgaggag ctggccgagg tggatgtgga ctggctgatc gccgagcgcc ccggcaaggt
    16621 aagaaccttg aaacagcatc cacgcaagaa caaaacggcc atcaacatcg aatacatgaa
    16681 agccagcatc cgggccaggg tggagcaccc atttcgcatc atcaagcgac agttcggctt
    16741 cgtgaaagcc agatacaagg ggttgctgaa aaacgataac caactggcga tgttattcac
    16801 gctggccaac ctgtttcggg cggaccaaat gatacgtcag tgggagagat ctcactaaaa
    16861 actggggata acgccttaaa tggcgaagaa acggtctaaa taggctgatt caaggcattt
    16921 acgggagaaa aaatcggctc aaacatgaag aaatgaaatg actgagtcag ccgagaagaa
    16981 tttccccgct tattcgcacc ttccctaggt actaaaacaa ttcatccagt aaaatataat
    17041 attttatttt ctcccaatca ggcttgatcc ccagtaagtc aaaaaatagc tcgacatact
    17101 gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa ggcaatgtca taccacttgt
    17161 ccgccctgcc gcttctccca agatcaataa agccacttac tttgccatct ttcacaaaga
    17221 tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc ctcttcgggc ttttccgtct
    17281 ttaaaaaatc atacagctcg cgcggatctt taaatggagt gtcttcttcc cagttttcgc
    17341 aatccacatc ggccagatcg ttattcagta agtaatccaa ttcggctaag cggctgtcta
    17401 agctattcgt atagggacaa tccgatatgt cgatggagtg aaagagcctg atgcactccg
    17461 catacagctc gataatcttt tcagggcttt gttcatcttc atactcttcc gagcaaagga
    17521 cgccatcggc ctcactcatg agcagattgc tccagccatc atgccgttca aagtgcagga
    17581 cctttggaac aggcagcttt ccttccagcc atagcatcat gtccttttcc cgttccacat
    17641 cataggtggt ccctttatac cggctgtccg tcatttttaa atataggttt tcattttctc
    17701 ccaccagctt atatacctta gcaggagaca ttccttccgt atcttttacg cagcggtatt
    17761 tttcgatcag ttttttcaat tccggtgata ttctcatttt agccatttat tatttccttc
    17821 ctcttttcta cagtatttaa agatacccca agaagctaat tataacaaga cgaactccaa
    17881 ttcactgttc cttgcattct aaaaccttaa ataccagaaa acagcttttt caaagttgtt
    17941 ttcaaagttg gcgtataaca tagtatcgac ggagccgatt ttgaaaccgc ggtgatcaca
    18001 ggcagcaacg ctctgtcatc gttacaatca acatgctacc ctccgcgaga tcatccgtgt
    18061 ttcaaacccg gcagcttagt tgccgttctt ccgaatagca tcggtaacat gagcaaagtc
    18121 tgccgcctta caacggctct cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg
    18181 agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt ggctggctgg
//
