LOCUS       sequence-268036-        8108 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Piggybac vector for bidirectional promoter reporter assay.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8108)
  TITLE     Madison Lab plasmids
REFERENCE   2  (bases 1 to 8108)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: BII-tdT-inv-GFP
FEATURES             Location/Qualifiers
     source          1..8108
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    257..481
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(join(518..1228,1229..1231))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             complement(join(518..1228,1229..1231))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(560..581)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     909..928
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1168..1189
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(1235..1255)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      1258..1267
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1323..1332
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1329..2756
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     CDS             2760..2780
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2843..3067
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    3102..3193
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(3134..3153)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    3207..3255
                     /note="synthetic polyadenylation signal"
     polyA_signal    3443..3491
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     3516..3535
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     misc_feature    join(3555..3592,3593..3658)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     repeat_region   4199..4233
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(4724..4740)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4724..4740)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4737..4759)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4748..4764
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4772..4778,4779..4796,4797..4802))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4817..4838
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4955..4972)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5126..5714)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5206..5225)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5885..6676,6677..6745))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6508..6527
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6746..6850)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6876..7331)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7013..7034)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7225..7244
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7458..7480
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7472..7489
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7473..7489
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   7619..7681
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
ORIGIN
        1 gtatggcttt ttccccgtat ccccccaggt gtctgcaggc tcaaagagca gcgagaagcg
       61 ttcagaggaa agcgatcccg tgccaccttc cccgtgcccg ggctgtcccc gcacgctgcc
      121 ggctcgggga tgcgggggga gcgccggacc ggagcggagc cccgggcggc tcgctgctgc
      181 cccctagcgg gggagggacg taattacatc cctgggggct ttgggggggg gctgtccctc
      241 tagaaggcct aacaggccat agagcccacc gcatccccag catgcctgct attgtcttcc
      301 caatcctccc ccttgctgtc ctgccccacc ccacccccca gaatagaatg acacctactc
      361 agacaatgcg atgcaatttc ctcattttat taggaaagga cagtgggagt ggcaccttcc
      421 agggtcaagg aaggcacggg ggaggggcaa acaacagatg gctggcaact agaaggcaca
      481 gtcgaggctg atcagcggtt taaacttaag ctagcttctt gtacagctcg tccatgccga
      541 gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg
      601 ggtctttgct cagggcggac tgggtgctca ggtagtggtt gtcgggcagc agcacggggc
      661 cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga
      721 tgttgtggcg gatcttgaag ttcaccttga tgccgttctt ctgcttgtcg gccatgatat
      781 agacgttgtg gctgttgtag ttgtactcca gcttgtgccc caggatgttg ccgtcctcct
      841 tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg aacttcacct
      901 cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc
      961 cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg tagcggctga
     1021 agcactgcac gccgtaggtc agggtggtca cgagggtggg ccagggcacg ggcagcttgc
     1081 cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc
     1141 cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg atgggcacca
     1201 ccccggtgaa cagctcctcg cccttgctca cgcccacctt gcgcttcttc ttggggccca
     1261 tggtggcacc ggtcgacgcg gccgcctagg aagcttggca ttccggtact gttgccaact
     1321 cggccaccat ggtgagcaag ggcgaggagg tcatcaaaga gttcatgcgc ttcaaggtgc
     1381 gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     1441 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc ctgcccttcg
     1501 cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg aagcaccccg
     1561 ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     1621 tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg caggacggca
     1681 cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc cccgtaatgc
     1741 agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc
     1801 tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac ctggtggagt
     1861 tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac tacgtggaca
     1921 ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgagcgct
     1981 ccgagggccg ccaccacctg ttcctggggc atggcaccgg cagcaccggc agcggcagct
     2041 ccggcaccgc ctcctccgag gacaacaaca tggccgtcat caaagagttc atgcgcttca
     2101 aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc gagggcgagg
     2161 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc ggccccctgc
     2221 ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg tacgtgaagc
     2281 accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc aagtgggagc
     2341 gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc tccctgcagg
     2401 acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc gacggccccg
     2461 taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac ccccgcgacg
     2521 gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc cactacctgg
     2581 tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc tactactacg
     2641 tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
     2701 agcgctccga gggccgccac cacctgttcc tgtacggcat ggacgagctg tacaagggcc
     2761 ccaagaagaa gcgcaaggtg ggcgcctaag ctaagcactt agtggccgtc gatcgtttaa
     2821 agggaggtag tgagtcgagc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     2881 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     2941 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
     3001 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
     3061 tctatggcgc gtctagctgt agtcatgcgg tacctatcga tagagaaatg ttctggcacc
     3121 tgcacttgca ctggggacag cctattttgc tagtttgttt tgtttcgttt tgttttgatg
     3181 gagagcgtat gttagtacta tcgattcaca caaaaaacca acacacagat gtaatgaaaa
     3241 taaagatatt ttattgcggc cgtggcggcc gaatatgcta tggcagggcc tgccgccccg
     3301 acgttggctg cgagccctgg gccttcaccc gaacttgggg ggtggggtgg ggaaaaggaa
     3361 gaaacgcggg cgtattggcc ccaatggggt ctcggtgggg tatcgacaga gtgccagccc
     3421 tgggaccgaa ccccgcgttt atgaacaaac gacccaacac ccgtgcgttt tattctgtct
     3481 ttttattgcc gtcatagcgc gggttccttc cggtattgtc tccttccgtg tttcagttag
     3541 cctcccccat gcatactgga aagaccgcga agagtttgtc ctcaaccgcg agctgtggaa
     3601 aaaaaaggga caggataagt atgacatcat caaggaaacc ctggactact gcgccctacc
     3661 gctgcagcct accaggcctt ttccccgtat ccccccaggt gtctgcaggc tcaaagagca
     3721 gcgagaagcg ttcagaggaa agcgatcccg tgccaccttc cccgtgcccg ggctgtcccc
     3781 gcacgctgcc ggctcgggga tgcgggggga gcgccggacc ggagcggagc cccgggcggc
     3841 tcgctgctgc cccctagcgg gggagggacg taattacatc cctgggggct ttgggggggg
     3901 gctgtccctc tagcacacat tccacagatc tataacaaga aaatatatat ataataagtt
     3961 atcacgtaag tagaacatga aataacaata taattatcgt atgagttaaa tcttaaaagt
     4021 cacgtaaaag ataatcatgc gtcattttga ctcacgcggt cgttatagtt caaaatcagt
     4081 gacacttacc gcattgacaa gcacgcctca cgggagctcc aagcggcgac tgagatgtcc
     4141 taaatgcaca gcgacggatt cgcgctattt agaaagagag agcaatattt caagaatgca
     4201 tgcgtcaatt ttacgcagac tatctttcta gggttaatct agctgcatca ggatcatatc
     4261 gtcgggtctt ttttccggct cagtcatcgc ccaagctggc gctatctggg catcggggag
     4321 gaagaagccc gtgccttttc ccgcgaggtt gaagcggcat ggaaagagtt tgccgaggat
     4381 gactgctgct gcattgacgt tgagcgaaaa cgcacgttta ccatgatgat tcgggaaggt
     4441 gtggccatgc acgcctttaa cggtgaactg ttcgttcagg ccacctggga taccagttcg
     4501 tcgcggcttt tccggacaca gttccggatg gtcagcccga agcgcatcag caacccgaac
     4561 aataccggcg acagccggaa ctgccgtgcc ggtgtgcaga ttaatgacag cggtgcggcg
     4621 ctgggatatt acgtcagcga ggacgggtat cctggctgga tgccgcagaa atggacatgg
     4681 ataccccgtg agttacccgg cgggcgcgct tggcgtaatc atggtcatag ctgtttcctg
     4741 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     4801 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     4861 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     4921 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     4981 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     5041 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     5101 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     5161 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     5221 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     5281 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     5341 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     5401 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     5461 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     5521 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     5581 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     5641 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     5701 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     5761 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     5821 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     5881 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     5941 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     6001 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     6061 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     6121 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     6181 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     6241 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     6301 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     6361 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     6421 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     6481 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     6541 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     6601 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     6661 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     6721 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     6781 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     6841 gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt
     6901 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     6961 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     7021 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     7081 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     7141 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     7201 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     7261 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     7321 acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     7381 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     7441 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcctc
     7501 gttcattcac gtttttgaac ccgtggagga cgggcagact cgcggtgcaa atgtgtttta
     7561 cagcgtgatg gagcagatga agatgctcga cacgctgcag aacacgcagc tagattaacc
     7621 ctagaaagat aatcatattg tgacgtacgt taaagataat catgtgtaaa attgacgcat
     7681 gtgttttatc ggtctgtata tcgaggttta tttattaatt tgaatagata ttaagtttta
     7741 ttatatttac acttacatac taataataaa ttcaacaaac aatttattta tgtttattta
     7801 tttattaaaa aaaacaaaaa ctcaaaattt cttctataaa gtaacaaaac ttttatcgaa
     7861 ttctttttcc ccgtatcccc ccaggtgtct gcaggctcaa agagcagcga gaagcgttca
     7921 gaggaaagcg atcccgtgcc accttccccg tgcccgggct gtccccgcac gctgccggct
     7981 cggggatgcg gggggagcgc cggaccggag cggagccccg ggcggctcgc tgctgccccc
     8041 tagcggggga gggacgtaat tacatccctg ggggctttgg gggggggctg tccccgtgag
     8101 ctccattg
//
