LOCUS       sequence-268041-        7318 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human Retinoic Acid Receptor-gamma and Human Retinoid X 
            Receptor-alpha in pcDNA3.1(+) plasmid expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7318)
  AUTHORS   Zolfaghari R, Mattie FJ, Wei CH, Chisholm DR, Whiting A, Ross AC
  TITLE     CYP26A1 gene promoter is a useful tool for reporting RAR-mediated 
            retinoid activity.
  JOURNAL   Anal Biochem. 2019 Jul 15;577:98-109. doi: 10.1016/j.ab.2019.04.022.
            Epub 2019 Apr 27.
  PUBMED    31039331
REFERENCE   2  (bases 1 to 7318)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem."; date: "2019-07-15"; volume: "577:98-109. doi"; pages: " 
            10.1016/j.ab.2019.04.022. Epub 2019 Apr 27"
COMMENT     Sequence Label: pcDNA3.1-hRAR-gamma.hRXR-alpha
FEATURES             Location/Qualifiers
     source          1..7318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      901..910
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(2382..2399)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2388..2612
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2658..3086
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2745..2764)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2955..2976
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3095..3115)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3113..3410
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     3342..3361
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     regulatory      3437..3446
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    4991..5112
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5028..5047)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5082..5101
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5161..5177)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5161..5177)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5174..5196)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5185..5201
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5209..5215,5216..5233,5234..5239))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5254..5275
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5392..5409)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5563..6151)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5643..5662)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6322..7113,7114..7182))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6945..6964
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7183..7287)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gccaccatgg ccaccaataa ggagcgactc tttgcggctg gtgccctggg gcctggatct
      961 ggctacccag gggcaggttt ccccttcgcc ttcccagggg cactcagggg gtctccgcct
     1021 ttcgagatgc tgagccctag cttccggggc ctgggccagc ctgacctccc caaggagatg
     1081 gcctctctgt cggtggagac acagagcacc agctcagagg agatggtgcc cagctcgccc
     1141 tcgccccctc cgcctcctcg ggtctacaag ccatgcttcg tgtgcaatga caagtcctct
     1201 ggctaccact atggggtcag ctcttgtgaa ggctgcaagg gcttctttcg ccgaagcatc
     1261 cagaagaaca tggtgtacac gtgtcaccgc gacaaaaact gtatcatcaa caaggtgacc
     1321 aggaatcgct gccagtactg ccggctacag aagtgcttcg aagtgggcat gtccaaggaa
     1381 gctgtgcgaa atgaccggaa caagaagaag aaagaggtga aggaagaagg gtcacctgac
     1441 agctatgagc tgagccctca gttagaagag ctcatcacca aggtcagcaa agcccatcag
     1501 gagactttcc cctcgctctg ccagctgggc aagtatacca cgaactccag tgcagaccac
     1561 cgcgtgcagc tggatctggg gctgtgggac aagttcagtg agctggctac caagtgcatc
     1621 atcaagatcg tggagtttgc caagcggttg cctggcttta cagggctcag cattgctgac
     1681 cagatcactc tgctcaaagc tgcctgccta gatatcctga tgctgcgtat ctgcacaagg
     1741 tacaccccag agcaggacac catgaccttc tccgacgggc tgaccctgaa ccggacccag
     1801 atgcacaatg ccggcttcgg gcccctcaca gaccttgtct ttgcctttgc tgggcagctc
     1861 ctgcccctgg agatggatga caccgagaca gggctgctca gcgccatctg cctcatctgc
     1921 ggagaccgca tggacctgga ggagcccgaa aaagtggaca agctgcagga gccactgctg
     1981 gaagccctga ggctgtacgc ccggcgccgg cggcccagcc agccctacat gttcccaagg
     2041 atgctaatga aaatcaccga cctccggggc atcagcacta agggagctga aagggccatt
     2101 actctgaaga tggagattcc aggcccgatg cctcccttaa tccgagagat gctggagaac
     2161 cctgaaatgt ttgaggatga ctcctcgcag cctggtcccc accccaatgc ctctagcgag
     2221 gatgaggttc ctgggggcca gggcaaaggg ggcctgaagt ccccagcctg aagcttggta
     2281 ccgagctcgg atccactagt ccagtgtggt ggaattctgc agatatccag cacagtggcg
     2341 gccgctcgag tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
     2401 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     2461 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     2521 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     2581 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     2641 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     2701 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     2761 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     2821 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     2881 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     2941 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     3001 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     3061 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
     3121 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     3181 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     3241 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     3301 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     3361 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggatctgca
     3421 gaattcacta gtgattgcca ccatggacac caaacatttc ctgccgctcg atttctccac
     3481 ccaggtgaac tcctccctca cctccccgac ggggcgaggc tccatggctg ccccctcgct
     3541 gcacccgtcc ctggggcctg gcatcggctc cccgggacag ctgcattctc ccatcagcac
     3601 cctgagctcc cccatcaacg gcatgggccc gcctttctcg gtcatcagct cccccatggg
     3661 cccccactcc atgtcggtgc ccaccacacc caccctgggc ttcagcactg gcagccccca
     3721 gctcagctca cctatgaacc ccgtcagcag cagcgaggac atcaagcccc ccctgggcct
     3781 caatggcgtc ctcaaggtcc ccgcccaccc ctcaggaaac atggcttcct tcaccaagca
     3841 catctgcgcc atctgcgggg accgctcctc aggcaagcac tatggagtgt acagctgcga
     3901 ggggtgcaag ggcttcttca agcggacggt gcgcaaggac ctgacctaca cctgccgcga
     3961 caacaaggac tgcctgattg acaagcggca gcggaaccgg tgccagtact gccgctacca
     4021 gaagtgcctg gccatgggca tgaagcggga agccgtgcag gaggagcggc agcgtggcaa
     4081 ggaccggaac gagaatgagg tggagtcgac cagcagcgcc aacgaggaca tgccggtgga
     4141 gaggatcctg gaggctgagc tggccgtgga gcccaagacc gagacctacg tggaggcaaa
     4201 catggggctg aaccccagct cgccgaacga ccctgtcacc aacatttgcc aagcagccga
     4261 caaacagctt ttcaccctgg tggagtgggc caagcggatc ccacacttct cagagctgcc
     4321 cctggacgac caggtcatcc tgctgcgggc aggctggaat gagctgctca tcgcctcctt
     4381 ctcccaccgc tccatcgccg tgaaggacgg gatcctcctg gccaccgggc tgcacgtcca
     4441 ccggaacagc gcccacagcg caggggtggg cgccatcttt gacagggtgc tgacggagct
     4501 tgtgtccaag atgcgggaca tgcagatgga caagacggag ctgggctgcc tgcgcgccat
     4561 cgtcctcttt aaccctgact ccaaggggct ctcgaacccg gccgaggtgg aggcgctgag
     4621 ggagaaggtc tatgcgtcct tggaggccta ctgcaagcac aagtacccag agcagccggg
     4681 aaggttcgct aagctcttgc tccgcctgcc ggctctgcgc tccatcgggc tcaaatgcct
     4741 ggaacatctc ttcttcttca agctcatcgg ggacacaccc attgacacct tccttatgga
     4801 gatgctggag gcgccgcacc aaatgactta gttcgaaatg accgaccaag cgacgcccaa
     4861 cctgccatca cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     4921 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     4981 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     5041 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     5101 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     5161 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     5221 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     5281 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     5341 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     5401 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     5461 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     5521 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     5581 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     5641 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     5701 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     5761 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     5821 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     5881 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     5941 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     6001 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     6061 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     6121 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     6181 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     6241 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     6301 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     6361 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     6421 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     6481 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     6541 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     6601 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     6661 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     6721 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     6781 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     6841 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     6901 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     6961 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     7021 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     7081 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     7141 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     7201 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     7261 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtc
//
