LOCUS       sequence-268053-        6990 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Full Length promoter of the human CYP26A1 gene in 
            pGL3-Basic-luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6990)
  AUTHORS   Zolfaghari R, Ross AC
  TITLE     Hepatocyte nuclear factor 4alpha (HNF4alpha) in coordination with 
            retinoic acid receptors increases all-trans-retinoic acid-dependent 
            CYP26A1 gene expression in HepG2 human hepatocytes.
  JOURNAL   J Cell Biochem. 2014 Oct;115(10):1740-51. doi: 10.1002/jcb.24839.
  PUBMED    24819304
REFERENCE   2  (bases 1 to 6990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/jcb"; 
            journalName: "J Cell Biochem"; date: "2014-10"; volume: "115"; 
            issue: "10"; pages: "1740-51"
COMMENT     Sequence Label: pGL3-Basic-hCYP26A1-FL- luciferase
FEATURES             Location/Qualifiers
     source          1..6990
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      2254..2263
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2260..3912
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(2326..2344)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    3953..4074
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3964..3983)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4018..4037
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4322..4339)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4493..5081)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4573..4592)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5252..6043,6044..6112))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5875..5894
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6113..6217)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6244..6699
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6331..6350)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6541..6562
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    6830..6878
                     /note="synthetic polyadenylation signal"
     misc_feature    6892..6983
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     6932..6951
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctaa gtaagcttgt
       61 acaatagatt aaaacgtttt tttccctgcg gcaatttcca gcgtgcaggc cccgctcatc
      121 ttgtgacccg gcttgagctg aacttggcga ggtggccccg cagagttcac tcggatgtca
      181 cggtccggcc tgcaggggtc acaggcgggt caggcccgag gattgggaat gggccccagg
      241 tcacgtcccc attcgtcggc tttgcacacc ggccggaggc gctgtccaga aaggggcgga
      301 cccgggattt ctgagaatta cctccagcgc gattggcctg gcttcctgca gtggccagag
      361 gttggttcgc cgtgtttaca gccaccaaat cgtttgccgg agactgaaac cctgcccaga
      421 gcctctgaaa cactgggcag gttatgggga aacgctgacc ccccgaaaca ctccctgtgg
      481 cggcgactct ggcttggaga gtcgctagga ttgtgcgggt ccctccgacc tctgcagata
      541 tttcctgata cctccgggtt tggggactac cgcgagaagg gcaacgcatt taaactctca
      601 ggagcagagt tttccacctc caagggacgc cttccaaggc gtgcggcgtg cttctgccag
      661 agacagtgtt cagttttaaa caaatgcagg tcattctgtt tcattttcgg cgccgcaggt
      721 gcgttcagcg cccaaaccca ggtagcgtgt gaccctgact ggtcccgagc cgggaagctc
      781 cggcaggtgg agagcagaga gagcagagag agcaggtgcg cgtcccgcag cgtctgagct
      841 ccgggcattg gcagctcagt agcagctgat tgtagatggg caaagtacag aagacttccc
      901 agtacggatc ccttcacccc tcccattagg ggtcggtaga gccctgagtt tagggccgag
      961 caccccctca cctggatgag gacagtggct ttactctggg tgggacttgg gagagaggag
     1021 gaggactcca gtgcccggtg taccggacac ccaggtaacg cagtctaccg tgcctggagc
     1081 tgggaagagt agttgcaacc ggagccccct tttgggaact cactaccctg aggcttagac
     1141 aggtcgggaa tgatcgcctc ccggttaagg aggaggaaac aggcacagtg atgtgtggtg
     1201 acttgcccaa ggtcacacgc agtacagatg gagccgggcc tcggagccag gactcctggg
     1261 ctccccttcc tcttataccg tctctttatc ctgccagggc taaggacccc cttccctgct
     1321 gtccagctgg ggcgccaagc agcagggccc tccgaatagg cgagctgtgc ttggatctca
     1381 gagacgggca ccgactgccg agccccagac gaagtgcccc agggtcgggg atcaggcttg
     1441 tgcagggtag cacctgggga cctgagggac ctggaggcct tcttgtatcc tgaagtagga
     1501 ccttctaaga cttcatgagt cctggccccc ttgcaggggt tggggagctg gggatcccag
     1561 atctgcctat tgcgcccgat gccccgaggc tctctcttgg actctggccc tgagttcttc
     1621 tgcgcgatcc ttcggagacg tctggaggcc tgctttatgc atctctcttg gacctcagtt
     1681 tccccacacg tgggaggagg cagctggacg attcctgaaa ggactttccc ttgcttcctc
     1741 atcacgtgga agagagccca cccggcacct ggaaatggaa agccagtgaa ggctgctttg
     1801 ggccggggca gcgggtggga ccgggcggga gggattccaa agagaccgcc gggaaggcta
     1861 gagcttggaa ttccggctcc tcggagtcct ggccctcccc caccgccgcc tcggagctca
     1921 gcacaccttg gatgggggag gcgggcagct cctagccccg caccccagga ggcgcgctcg
     1981 gagggaagcc gccaccgcgc cgcctctgcc tcggcgcgga acaaacggtt aaagattttg
     2041 ggcagcgcct cgcgggggga ggagccaggg gcccaatccg caattaaaga tgaactttgg
     2101 gtgaactaat tgtctgacca aggtaacgtg ggcagcaacc tgggccgcct ataaagcggc
     2161 agcgccgtgg ggtttgaagc gctggcggcg gcggcaggtg gcgcgggagg tcgcggcgcg
     2221 cgtgaagctt ggcattccgg tactgttggt aaagccacca tggaagacgc caaaaacata
     2281 aagaaaggcc cggcgccatt ctatccgctg gaagatggaa ccgctggaga gcaactgcat
     2341 aaggctatga agagatacgc cctggttcct ggaacaattg cttttacaga tgcacatatc
     2401 gaggtggaca tcacttacgc tgagtacttc gaaatgtccg ttcggttggc agaagctatg
     2461 aaacgatatg ggctgaatac aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa
     2521 ttctttatgc cggtgttggg cgcgttattt atcggagttg cagttgcgcc cgcgaacgac
     2581 atttataatg aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc
     2641 gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa
     2701 aaaattatta tcatggattc taaaacggat taccagggat ttcagtcgat gtacacgttc
     2761 gtcacatctc atctacctcc cggttttaat gaatacgatt ttgtgccaga gtccttcgat
     2821 agggacaaga caattgcact gatcatgaac tcctctggat ctactggtct gcctaaaggt
     2881 gtcgctctgc ctcatagaac tgcctgcgtg agattctcgc atgccagaga tcctattttt
     2941 ggcaatcaaa tcattccgga tactgcgatt ttaagtgttg ttccattcca tcacggtttt
     3001 ggaatgttta ctacactcgg atatttgata tgtggatttc gagtcgtctt aatgtataga
     3061 tttgaagaag agctgtttct gaggagcctt caggattaca agattcaaag tgcgctgctg
     3121 gtgccaaccc tattctcctt cttcgccaaa agcactctga ttgacaaata cgatttatct
     3181 aatttacacg aaattgcttc tggtggcgct cccctctcta aggaagtcgg ggaagcggtt
     3241 gccaagaggt tccatctgcc aggtatcagg caaggatatg ggctcactga gactacatca
     3301 gctattctga ttacacccga gggggatgat aaaccgggcg cggtcggtaa agttgttcca
     3361 ttttttgaag cgaaggttgt ggatctggat accgggaaaa cgctgggcgt taatcaaaga
     3421 ggcgaactgt gtgtgagagg tcctatgatt atgtccggtt atgtaaacaa tccggaagcg
     3481 accaacgcct tgattgacaa ggatggatgg ctacattctg gagacatagc ttactgggac
     3541 gaagacgaac acttcttcat cgttgaccgc ctgaagtctc tgattaagta caaaggctat
     3601 caggtggctc ccgctgaatt ggaatccatc ttgctccaac accccaacat cttcgacgca
     3661 ggtgtcgcag gtcttcccga cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg
     3721 gagcacggaa agacgatgac ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca
     3781 accgcgaaaa agttgcgcgg aggagttgtg tttgtggacg aagtaccgaa aggtcttacc
     3841 ggaaaactcg acgcaagaaa aatcagagag atcctcataa aggccaagaa gggcggaaag
     3901 atcgccgtgt aattctagag tcggggcggc cggccgcttc gagcagacat gataagatac
     3961 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     4021 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     4081 aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc
     4141 aagtaaaacc tctacaaatg tggtaaaatc gataaggatc cgtcgaccga tgcccttgag
     4201 agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact
     4261 tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tcttccgctt
     4321 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     4381 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     4441 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     4501 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     4561 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     4621 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     4681 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     4741 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     4801 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     4861 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     4921 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     4981 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     5041 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     5101 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     5161 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     5221 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     5281 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     5341 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     5401 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     5461 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     5521 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     5581 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     5641 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     5701 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     5761 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     5821 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     5881 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     5941 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     6001 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     6061 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     6121 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     6181 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     6241 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     6301 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     6361 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     6421 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
     6481 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     6541 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
     6601 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     6661 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttg ccattcgcca ttcaggctgc
     6721 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag cccaagctac
     6781 catgataagt aagtaatatt aaggtacggg aggtacttgg agcggccgca ataaaatatc
     6841 tttattttca ttacatctgt gtgttggttt tttgtgtgaa tcgatagtac taacatacgc
     6901 tctccatcaa aacaaaacga aacaaaacaa actagcaaaa taggctgtcc ccagtgcaag
     6961 tgcaggtgcc agaacatttc tctatcgata
//
