LOCUS       sequence-268063-        6787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human full-length promoter of retinoic acid receptor-beta gene in 
            pGL3-Basic-luciferase plasmid vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6787)
  AUTHORS   Zolfaghari R, Mattie FJ, Wei CH, Chisholm DR, Whiting A, Ross AC
  TITLE     CYP26A1 gene promoter is a useful tool for reporting RAR-mediated 
            retinoid activity.
  JOURNAL   Anal Biochem. 2019 Jul 15;577:98-109. doi: 10.1016/j.ab.2019.04.022.
            Epub 2019 Apr 27.
  PUBMED    31039331
REFERENCE   2  (bases 1 to 6787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem."; date: "2019-07-15"; volume: "577:98-109. doi"; pages: " 
            10.1016/j.ab.2019.04.022. Epub 2019 Apr 27"
COMMENT     Sequence Label: pGL3-basic-hRAR-beta-P-FL-luciferase
FEATURES             Location/Qualifiers
     source          1..6787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      2051..2060
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2057..3709
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(2123..2141)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    3750..3871
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3761..3780)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3815..3834
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4119..4136)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4290..4878)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4370..4389)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5049..5840,5841..5909))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5672..5691
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5910..6014)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6041..6496
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6128..6147)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6338..6359
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    6627..6675
                     /note="synthetic polyadenylation signal"
     misc_feature    6689..6780
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     6729..6748
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gaattcgatt ccaatgcaca ttccaacact
       61 atccacaaaa aggagactgg agcagtgctc ttccctgcat tgggcaagga gactctccct
      121 ccctgcctaa ccacttgcct gccctgtttt gtgggagaat tacaagtaaa tgctacagag
      181 gcagtggaga aaaaagggtg ttttaattcc tctccagagt ttcctttatt tgatgtatgt
      241 tgcatccttt aaacaagttg tgcaaaatgg ctgcagggta gattggctct cccttttaaa
      301 gctctccatc cggctgggtt tatttgtaaa tactgcatct atccttctta gtgttttagg
      361 actggctgga aagactcttc ttcctgtagg ttgggtcagt gtgagagatc taaaaaatca
      421 ttttccctta aaattactgt attttaataa aaggattggg caggggctgg aatgagagaa
      481 aactggtcct tcaaaatgta aaactgtcat acttaaacca gtttacaaaa tatgcgttta
      541 attatgtggt gggatgtgtg taggtgtatg atgagagagg caaccaacat ggctatttgg
      601 ggtgcaagga tgtgggaaca ggcaagtaat tttcacattg gactttcatc ctagggagct
      661 gggttctagt cacagctctg agctgtgtga ccttgggtag gtctcatctc cccggggttt
      721 tgtttcacca gttgaacagt atgaggatga gtcacagcta acatttgttc catgatattt
      781 acccagcacc atacaagtgt tatttctgtc ctcccagtta acactgacgt gggtagtatt
      841 atatgcccat tttacagatg aggaaactga agcctgaaga agttaaatac ttatcccaga
      901 acacacagct ggtaagtggc agacctggaa ttggaatcta gttcagtttg attccccaac
      961 ccatgctctt gaccactata ctgttttttc aagtccagat ctgaaatctc attttctgtg
     1021 tggctgtgtg tttgggacag gggtaaccaa ttcctgacta ctctatatgc tgcatagaac
     1081 ctggagagga tttttcaaag taaatgaatc tcgaaagctg gattgcagag caaacgagtg
     1141 cagtcaattc agccaggggc ttgcaagagg gagaaagaga aaaagactgt ggaatggaaa
     1201 gtttcccaac ccaagccttt cccaaggggt agccattctc tgttctacag tttagggctt
     1261 gcatgtgctt tttctggagt ggaaaaatac ataagttata aggaatttaa cagacagaaa
     1321 ggcgcacaga ggaatttaaa gtgtgggctg gggggcgagg cggtgggcgg gaggcgagcg
     1381 ggcgcaggcg gaacaccgtt ttccaagcta agccgccgca aataaaaagg cgtaaaggga
     1441 gagaagttgg tgctcaacgt gagccaggag cagcgtcccg gctcctcccc tgctcatttt
     1501 aaaagcactt cttgtattgt ttttaaggtg agaaatagga aagaaaacgc cggcttgtgc
     1561 gctcgctgcc tgcctctctg gctgtctgct tttgcagggc tgctgggagt ttttaagctc
     1621 tgtgagaatc ctgggagttg gtgatgtcag actagttggg tcatttgaag gttagcagcc
     1681 cgggtagggt tcaccgaaag ttcactcgca tatattaggc aattcaatct ttcattctgt
     1741 gtgacagaag tagtaggaag tgagctgttc agaggcagga gggtctattc tttgccaaag
     1801 gggggaccag aattccccca tgcgagctgt ttgaggactg ggatgccgag aacgcgagcg
     1861 atccgagcag ggtttgtctg ggcaccgtcg gggtaggatc cggaacgcat tcggaaggct
     1921 ttttgcaagc atttacttgg aaggagaact tgggatcaat cactagtgaa ttcgcggccg
     1981 cctgcaggtc gaccataggg ctcgagatct gcgatctaag taagcttggc attccggtac
     2041 tgttggtaaa gccaccatgg aagacgccaa aaacataaag aaaggcccgg cgccattcta
     2101 tccgctggaa gatggaaccg ctggagagca actgcataag gctatgaaga gatacgccct
     2161 ggttcctgga acaattgctt ttacagatgc acatatcgag gtggacatca cttacgctga
     2221 gtacttcgaa atgtccgttc ggttggcaga agctatgaaa cgatatgggc tgaatacaaa
     2281 tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc
     2341 gttatttatc ggagttgcag ttgcgcccgc gaacgacatt tataatgaac gtgaattgct
     2401 caacagtatg ggcatttcgc agcctaccgt ggtgttcgtt tccaaaaagg ggttgcaaaa
     2461 aattttgaac gtgcaaaaaa agctcccaat catccaaaaa attattatca tggattctaa
     2521 aacggattac cagggatttc agtcgatgta cacgttcgtc acatctcatc tacctcccgg
     2581 ttttaatgaa tacgattttg tgccagagtc cttcgatagg gacaagacaa ttgcactgat
     2641 catgaactcc tctggatcta ctggtctgcc taaaggtgtc gctctgcctc atagaactgc
     2701 ctgcgtgaga ttctcgcatg ccagagatcc tatttttggc aatcaaatca ttccggatac
     2761 tgcgatttta agtgttgttc cattccatca cggttttgga atgtttacta cactcggata
     2821 tttgatatgt ggatttcgag tcgtcttaat gtatagattt gaagaagagc tgtttctgag
     2881 gagccttcag gattacaaga ttcaaagtgc gctgctggtg ccaaccctat tctccttctt
     2941 cgccaaaagc actctgattg acaaatacga tttatctaat ttacacgaaa ttgcttctgg
     3001 tggcgctccc ctctctaagg aagtcgggga agcggttgcc aagaggttcc atctgccagg
     3061 tatcaggcaa ggatatgggc tcactgagac tacatcagct attctgatta cacccgaggg
     3121 ggatgataaa ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga aggttgtgga
     3181 tctggatacc gggaaaacgc tgggcgttaa tcaaagaggc gaactgtgtg tgagaggtcc
     3241 tatgattatg tccggttatg taaacaatcc ggaagcgacc aacgccttga ttgacaagga
     3301 tggatggcta cattctggag acatagctta ctgggacgaa gacgaacact tcttcatcgt
     3361 tgaccgcctg aagtctctga ttaagtacaa aggctatcag gtggctcccg ctgaattgga
     3421 atccatcttg ctccaacacc ccaacatctt cgacgcaggt gtcgcaggtc ttcccgacga
     3481 tgacgccggt gaacttcccg ccgccgttgt tgttttggag cacggaaaga cgatgacgga
     3541 aaaagagatc gtggattacg tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg
     3601 agttgtgttt gtggacgaag taccgaaagg tcttaccgga aaactcgacg caagaaaaat
     3661 cagagagatc ctcataaagg ccaagaaggg cggaaagatc gccgtgtaat tctagagtcg
     3721 gggcggccgg ccgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac
     3781 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt
     3841 tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt
     3901 tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg
     3961 taaaatcgat aaggatccgt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct
     4021 tccggtgggc gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc
     4081 aactcgtagg acaggtgccg gcagcgctct tccgcttcct cgctcactga ctcgctgcgc
     4141 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     4201 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     4261 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     4321 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     4381 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     4441 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     4501 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     4561 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     4621 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     4681 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     4741 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     4801 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     4861 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     4921 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     4981 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     5041 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     5101 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     5161 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     5221 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     5281 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     5341 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     5401 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     5461 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     5521 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     5581 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     5641 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     5701 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     5761 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     5821 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     5881 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     5941 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     6001 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca
     6061 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     6121 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     6181 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     6241 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     6301 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     6361 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     6421 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     6481 ttaacgctta caatttgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
     6541 gtgcgggcct cttcgctatt acgccagccc aagctaccat gataagtaag taatattaag
     6601 gtacgggagg tacttggagc ggccgcaata aaatatcttt attttcatta catctgtgtg
     6661 ttggtttttt gtgtgaatcg atagtactaa catacgctct ccatcaaaac aaaacgaaac
     6721 aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga acatttctct
     6781 atcgata
//
