LOCUS       sequence-268067-       10181 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of eGFP (as control) tagged with 
            N-terminal miniTurbo-V5 for generating protein-proximity networks 
            using proximity-dependent biotinylation proteomics .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10181)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10181)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-eGFP_IDG-K
FEATURES             Location/Qualifiers
     source          1..10181
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..22
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(37..42,43..60,61..67)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    75..91
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     80..102
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     99..115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     99..115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     134..154
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        136..154
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        182..408
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             409..589
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    636..761
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1254..1487
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1672..1716
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1983..2099
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    2133..2153
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        2153..3364
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3201..3220
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(3368..3388)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      3389..3398
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3401..4171
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             4178..4219
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    4220..4244
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      4244..4253
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4250..4252,4253..4255,4256..4969)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4250..4252,4253..4255,4256..4966)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4556..4575)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4903..4924
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(4968..4992)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5022..5610
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5075..5095)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5493..5504)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        5624..6123
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5659..5682)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6056..6075
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6144..6743
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6144..6163)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6640..6660
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6881..7114
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7186..7307
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7223..7242)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7277..7296
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7347..7482
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7502..7521)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7503..7521)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7531..7548)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7531..7547)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7540..7562)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7689..8144
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7776..7795)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7986..8007
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8170..8274
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8275..8343,8344..9135)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      9306..9894
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9795..9814
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10048..10065
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
       61 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
      121 ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta
      181 atgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat
      241 gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat
      301 cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg
      361 ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg
      421 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
      481 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
      541 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
      601 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
      661 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
      721 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
      781 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
      841 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
      901 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
      961 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1021 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1081 agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     1141 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     1201 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     1261 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     1321 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     1381 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     1441 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     1501 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     1561 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     1621 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     1681 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     1741 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     1801 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     1861 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     1921 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta
     1981 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     2041 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta
     2101 tcgtccagtg tggtggaatt ctgcagatat cacaactttg tatagaaaag ttggcctccg
     2161 cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca cggcgagcgc tgccacgtca
     2221 gacgaagggc gcagcgagcg tcctgatcct tccgcccgga cgctcaggac agcggcccgc
     2281 tgctcataag actcggcctt agaaccccag tatcagcaga aggacatttt aggacgggac
     2341 ttgggtgact ctagggcact ggttttcttt ccagagagcg gaacaggcga ggaaaagtag
     2401 tcccttctcg gcgattctgc ggagggatct ccgtggggcg gtgaacgccg atgattatat
     2461 aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg gtcgcggttc
     2521 ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg ctggccgggg
     2581 ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg tggagagacc gccaagggct
     2641 gtagtctggg tccgcgagca aggttgccct gaactggggg ttggggggag cgcagcaaaa
     2701 tggcggctgt tcccgagtct tgaatggaag acgcttgtga ggcgggctgt gaggtcgttg
     2761 aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc ttcgctaatg
     2821 cgggaaagct cttattcggg tgagatgggc tggggcacca tctggggacc ctgacgtgaa
     2881 gtttgtcact gactggagaa ctcggtttgt cgtctgttgc gggggcggca gttatggcgg
     2941 tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg tcgtgacgtc
     3001 acccgttctg ttggcttata atgcagggtg gggccacctg ccggtaggtg tgcggtaggc
     3061 ttttctccgt cgcaggacgc agggttcggg cctagggtag gctctcctga atcgacaggc
     3121 gccggacctc tggtgagggg agggataagt gaggcgtcag tttctttggt cggttttatg
     3181 tacctatctt cttaagtagc tgaagctccg gttttgaact atgcgctcgg ggttggcgag
     3241 tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt caatatgtaa
     3301 ttttcagtgt tagactagta aattgtccgc taaattctgg ccgtttttgg cttttttgtt
     3361 agacggccaa cttttgtata caaagtgggc caccatggga atcccgctgc tgaacgctaa
     3421 acagattctg ggacagctgg acggcgggag cgtggcagtc ctgcctgtgg tcgactccac
     3481 caatcagtac ctgctggatc gaatcggcga gctgaagagt ggggatgctt gcattgcaga
     3541 atatcagcag gcagggagag gaagcagagg gaggaaatgg ttctctcctt ttggagctaa
     3601 cctgtacctg agtatgtttt ggcgcctgaa gcggggacca gcagcaatcg gcctgggccc
     3661 ggtcatcgga attgtcatgg cagaagcgct gcgaaagctg ggagcagaca aggtgcgagt
     3721 caaatggccc aatgacctgt atctgcagga tagaaagctg gcaggcatcc tggtggagct
     3781 ggccggaata acaggcgatg ctgcacagat cgtcattggc gccgggatta acgtggctat
     3841 gaggcgcgtg gaggaaagcg tggtcaatca gggctggatc acactgcagg aagcagggat
     3901 taacctggac aggaatactc tggccgctat gctgatccga gagctgcggg cagccctgga
     3961 actgttcgag caggaaggcc tggctccata tctgtcacgg tgggagaagc tggataactt
     4021 catcaataga cccgtgaagc tgatcattgg ggacaaagag attttcggga ttagccgggg
     4081 gattgataaa cagggagccc tgctgctgga acaggacgga gttatcaaac cctggatggg
     4141 cggagaaatc agtctgcggt ctgccgaaaa ggctagcggc aagcccatcc ccaaccccct
     4201 gctgggcctg gacagcacca caactttgta caaaaaagtt ggcgccacca tggtgagcaa
     4261 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa
     4321 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac
     4381 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac
     4441 cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt
     4501 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga
     4561 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat
     4621 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta
     4681 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt
     4741 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca
     4801 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac
     4861 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt
     4921 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaat caactttctt
     4981 gtacaaagtt gtgatatcca gcacagtggc ggccgctcga caatcaacct ctggattaca
     5041 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     5101 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     5161 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     5221 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     5281 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     5341 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     5401 tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga
     5461 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     5521 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     5581 gtcggatctc cctttgggcc gcctccccgc ctggaattct accgggtagg ggaggcgctt
     5641 ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca
     5701 agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc
     5761 tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc
     5821 ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg
     5881 cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag
     5941 cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg
     6001 ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc
     6061 tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt
     6121 tcgacctgca gcccaagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc
     6181 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca
     6241 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct
     6301 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg
     6361 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc
     6421 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc
     6481 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg
     6541 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc
     6601 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc
     6661 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca
     6721 tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg accgaaagga
     6781 gcgcacgacc ccatgcatct cgagggcccg gtacctttaa gaccaatgac ttacaaggca
     6841 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct agctcactcc
     6901 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     6961 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     7021 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     7081 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     7141 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     7201 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     7261 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     7321 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     7381 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     7441 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
     7501 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     7561 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     7621 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     7681 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     7741 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     7801 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     7861 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     7921 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     7981 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     8041 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     8101 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     8161 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     8221 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     8281 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     8341 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     8401 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     8461 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     8521 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     8581 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     8641 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     8701 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     8761 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     8821 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     8881 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     8941 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     9001 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     9061 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     9121 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     9181 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     9241 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     9301 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     9361 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     9421 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     9481 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     9541 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     9601 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     9661 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     9721 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     9781 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     9841 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     9901 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     9961 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
    10021 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
    10081 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
    10141 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa t
//
