LOCUS       sequence-268070-        6861 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV donor/transfer vector expressing CreLite system components, 
            PhyB-Delta-CreC and PIF6CreN, driven by CBh promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6861)
  AUTHORS   Yen ST, Trimmer KA, Aboul-Fettouh N, Mullen RD, Culver JC, Dickinson
            ME, Behringer RR, Eisenhoffer GT
  TITLE     CreLite: An optogenetically controlled Cre/loxP system using red 
            light.
  JOURNAL   Dev Dyn. 2020 Nov;249(11):1394-1403. doi: 10.1002/dvdy.232. Epub 
            2020 Aug 31.
  PUBMED    32745301
REFERENCE   2  (bases 1 to 6861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/dvdy.232"; 
            journalName: "Dev Dyn"; date: "2020-11"; volume: "249"; issue: "11";
            pages: "1394-1403"
COMMENT     Sequence Label: pAAV-CreLite
FEATURES             Location/Qualifiers
     source          1..6861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
     CDS             593..613
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3443..3499
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3503..3523
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4117..4165
                     /note="synthetic polyadenylation signal"
     repeat_region   4219..4348
                     /label=AAV2 ITR
     rep_origin      4420..4875
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4507..4526)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4717..4738
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4901..5005
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5006..5074,5075..5866)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5224..5243)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6037..6625
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6526..6545
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct
      181 ggaaagcttc gcccttaagc tagtatgcca agtacgcccc ctattgacgt caatgacggt
      241 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      301 tacatctact cgaggccacg ttctgcttca ctctccccat ctcccccccc tccccacccc
      361 caattttgta tttatttatt ttttaattat tttgtgcagc gatgggggcg aggggcgggg
      421 cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc
      481 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg
      541 agcgggatca gaatgatctg atatcatcga tgaattcgag ctcgccacca tgcccaagaa
      601 gaagaggaag gtggtttccg gagtcggggg tagtggcggt ggccgtggcg gtggccgtgg
      661 cggagaagaa gaaccgtcgt caagtcacac tcctaataac cgaagaggag gagaacaagc
      721 tcaatcgtcg ggaacgaaat ctctcagacc aagaagcaac actgaatcaa tgagcaaagc
      781 aattcaacag tacaccgtcg acgcaagact ccacgccgtt ttcgaacaat ccggcgaatc
      841 agggaaatca ttcgactact cacaatcact caaaacgacg acgtacggtt cctctgtacc
      901 tgagcaacag atcacagctt atctctctcg aatccagcga ggtggttaca ttcagccttt
      961 cggatgtatg atcgccgtcg atgaatccag tttccggatc atcggttaca gtgaaaacgc
     1021 cagagaaatg ttagggatta tgcctcaatc tgttcctact cttgagaaac ctgagattct
     1081 agctatggga actgatgtga gatctttgtt cacttcttcc agctcgattc tactcgagcg
     1141 tgctttcgtt gctcgagaga ttaccttgtt aaatccggtt tggatccatt ccaagaatac
     1201 tggtaaaccg ttttacgcca ttcttcatag gattgatgtt ggtgttgtta ttgatttaga
     1261 gccagctaga actgaagatc ctgcgctttc tattgctggt gctgttcaat cgcagaaact
     1321 cgcggttcgt gcgatttctc agttacaggc tcttcctggt ggagatatta agctgttgtg
     1381 tgacactgtc gtggaaagtg tgagggactt gactggttat gatcgtgtta tggtttataa
     1441 gtttcatgaa gatgagcatg gagaagttgt agctgagagt aaacgagatg atttagagcc
     1501 ttatattgga ctgcattatc ctgctactga tattcctcaa gcgtcaaggt tcttgtttaa
     1561 gcagaaccgt gtccgaatga tagtagattg caatgccaca cctgttcttg tggtccagga
     1621 cgataggcta actcagtcta tgtgcttggt tggttctact cttagggctc ctcatggttg
     1681 tcactctcag tatatggcta acatgggatc tattgcgtct ttagcaatgg cggttataat
     1741 caatggaaat gaagatgatg ggagcaatgt agctagtgga agaagctcga tgaggctttg
     1801 gggtttggtt gtttgccatc acacttcttc tcgctgcata ccgtttccgc taaggtatgc
     1861 ttgtgagttt ttgatgcagg ctttcggttt acagttaaac atggaattgc agttagcttt
     1921 gcaaatgtca gagaaacgtg ttttgagaac gcagacactg ttatgtgata tgcttctgcg
     1981 tgactcgcct gctggaattg ttacacagag tcccagtatc atggacttag tgaaatgtga
     2041 cggtgcagca tttctttacc acgggaagta ttacccgttg ggtgttgctc ctagtgaagt
     2101 tcagataaaa gatgttgtgg agtggttgct tgcgaatcat gcggattcaa ccggattaag
     2161 cactgatagt ttaggcgatg cggggtatcc cggtgcagct gcgttagggg atgctgtgtg
     2221 cggtatggca gttgcatata tcacaaaaag agactttctt ttttggtttc gatctcacac
     2281 tgcgaaagaa atcaaatggg gaggcgctaa gcatcatccg gaggataaag atgatgggca
     2341 acgaatgcat cctcgttcgt cctttcaggc ttttcttgaa gttgttaaga gccggagtca
     2401 gccatgggaa actgcggaaa tggatgcgat tcactcgctc cagcttattc tgagagactc
     2461 ttttaaagaa tctggtggtt ctgccggtgg ctccggttct ggctccagcg gtggcagctc
     2521 tggtgcgtcc ggcacgggta ctgcgggtgg cactggcagc ggttccggta ctggctctgg
     2581 caaccggaaa tggtttcccg cagaacctga agatgttcgc gattatcttc tatatcttca
     2641 ggcgcgcggt ctggcagtaa aaactatcca gcaacatttg ggccagctaa acatgcttca
     2701 tcgtcggtcc gggctgccac gaccaagtga cagcaatgct gtttcactgg ttatgcggcg
     2761 gatccgaaaa gaaaacgttg atgccggtga acgtgcaaaa caggctctag cgttcgaacg
     2821 cactgatttc gaccaggttc gttcactcat ggaaaatagc gatcgctgcc aggatatacg
     2881 taatctggca tttctgggga ttgcttataa caccctgtta cgtatagccg aaattgccag
     2941 gatcagggtt aaagatatct cacgtactga cggtgggaga atgttaatcc atattggcag
     3001 aacgaaaacg ctggttagca ccgcaggtgt agagaaggca cttagcctgg gggtaactaa
     3061 actggtcgag cgatggattt ccgtctctgg tgtagctgat gatccgaata actacctgtt
     3121 ttgccgggtc agaaaaaatg gtgttgccgc gccatctgcc accagccagc tatcaactcg
     3181 cgccctggaa gggatttttg aagcaactca tcgattgatt tacggcgcta aggatgactc
     3241 tggtcagaga tacctggcct ggtctggaca cagtgcccgt gtcggagccg cgcgagatat
     3301 ggcccgcgct ggagtttcaa taccggagat catgcaagct ggtggctgga ccaatgtaaa
     3361 tattgtcatg aactatatcc gtaacctgga tagtgaaaca ggggcaatgg tgcgcctgct
     3421 ggaagatggc gatggaagcg gagctactaa cttcagcctg ctgaagcagg ctggagacgt
     3481 ggaggagaac cctggaccta tgcccaagaa gaagaggaag gtgatgttct taccaaccga
     3541 ttattgttgc aggttaagcg atcaagagta tatggagctt gtgtttgaga atggccagat
     3601 tcttgcaaag ggccaaagat ccaacgtttc tctgcataat caacgtacca aatcgatcat
     3661 ggatttgtat gaggcagagt ataacgagga tttcatgaag agtatcatcc atggtggtgg
     3721 tggtgccatc acaaatctcg gggacacgca ggttgttcca caaagtcatg ttgctgctgc
     3781 ccatgaaaca aacatgttgg aaagcaataa acatgttgac ggtggttctg ccggtggctc
     3841 cggttctggc tccagcggtg gcagctctgg tgcgtccggc acgggtactg cgggtggcac
     3901 tggcagcggt tccggtactg gctctggcct gactgtgcac caaaacctgc ctgccctccc
     3961 tgtggatgcc acctctgatg aagtcaggaa gaacctgatg gacatgttca gggacaggca
     4021 ggccttctct gaacacacct ggaagatgct cctgtctgtg tgcagatcct gggctgcctg
     4081 gtgcaagctg aactgagaat tctctagact agagcgaata aaagatcttt attttcatta
     4141 gatctgtgtg ttggtttttt gtgtgatgca gcacgcgtta cgtagataag tagcatggcg
     4201 ggttaatcat taactacaag gaacccctag tgatggagtt ggccactccc tctctgcgcg
     4261 ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg
     4321 cggcctcagt gagcgagcga gcgcgcagct ggcgtaatag cgaagaggcc cgcaccgatc
     4381 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
     4441 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     4501 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     4561 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     4621 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     4681 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     4741 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     4801 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     4861 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     4921 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     4981 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     5041 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     5101 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     5161 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     5221 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     5281 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     5341 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     5401 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     5461 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     5521 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     5581 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     5641 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     5701 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     5761 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     5821 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     5881 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     5941 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     6001 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     6061 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     6121 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     6181 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     6241 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     6301 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     6361 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     6421 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     6481 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     6541 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     6601 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     6661 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     6721 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     6781 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     6841 ttggccgatt cattaatgca g
//
