LOCUS       sequence-268071-       12455 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector expressing CreLite, driven by CBh promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12455)
  AUTHORS   Yen ST, Trimmer KA, Aboul-Fettouh N, Mullen RD, Culver JC, Dickinson
            ME, Behringer RR, Eisenhoffer GT
  TITLE     CreLite: An optogenetically controlled Cre/loxP system using red 
            light.
  JOURNAL   Dev Dyn. 2020 Nov;249(11):1394-1403. doi: 10.1002/dvdy.232. Epub 
            2020 Aug 31.
  PUBMED    32745301
REFERENCE   2  (bases 1 to 12455)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/dvdy.232"; 
            journalName: "Dev Dyn"; date: "2020-11"; volume: "249"; issue: "11";
            pages: "1394-1403"
COMMENT     Sequence Label: pLenti-CreLite
FEATURES             Location/Qualifiers
     source          1..12455
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        20..298
                     /label=chicken beta-actin promoter
     intron          299..526
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     protein_bind    531..555
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             565..585
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3415..3471
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3475..3495
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    complement(4069..4093)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     enhancer        4153..4456
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4457..4660
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4611..4631
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             4688..5386
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             5396..5449
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5450..6049
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5450..5469)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5946..5966
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    complement(6050..6070)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    6083..6671
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6136..6156)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6554..6565)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6759..6992
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7064..7185
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7101..7120)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7155..7174
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7225..7360
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7380..7399)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7381..7399)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7409..7426)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7409..7425)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7418..7440)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7567..8022
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7654..7673)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7864..7885
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8048..8152
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8153..8221,8222..9013)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8371..8390)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9184..9772
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9673..9692
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9926..9943
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10060..10081
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10096..10101,10102..10119,10120..10126)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10134..10150
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10139..10161
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10158..10174
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10158..10174
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10193..10213
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10195..10213
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        10241..10467
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10468..10648
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10695..10820
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    11313..11546
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11731..11775
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    12041..12158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    12167..12187
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        join(12188..12455,1..18)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 gtcatcgcta ttaccatggt cgaggtgagc cccacgttct gcttcactct ccccatctcc
       61 cccccctccc cacccccaat tttgtattta tttatttttt wattattttg tgcagcgatg
      121 ggggcgggsg gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg
      181 cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc
      241 ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg
      301 agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc
      361 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      421 gggctgtaat tagctgagca agaggtaagg gtttaaggga tggttggttg gtggggtatt
      481 aatgtttaat tacctggagc acctgcctga aatcactttt tttcaggttg gcaagtttgt
      541 acaaaaaagc aggctgccac catgcccaag aagaagagga aggtggtttc cggagtcggg
      601 ggtagtggcg gtggccgtgg cggtggccgt ggcggagaag aagaaccgtc gtcaagtcac
      661 actcctaata accgaagagg aggagaacaa gctcaatcgt cgggaacgaa atctctcaga
      721 ccaagaagca acactgaatc aatgagcaaa gcaattcaac agtacaccgt cgacgcaaga
      781 ctccacgccg ttttcgaaca atccggcgaa tcagggaaat cattcgacta ctcacaatca
      841 ctcaaaacga cgacgtacgg ttcctctgta cctgagcaac agatcacagc ttatctctct
      901 cgaatccagc gaggtggtta cattcagcct ttcggatgta tgatcgccgt cgatgaatcc
      961 agtttccgga tcatcggtta cagtgaaaac gccagagaaa tgttagggat tatgcctcaa
     1021 tctgttccta ctcttgagaa acctgagatt ctagctatgg gaactgatgt gagatctttg
     1081 ttcacttctt cgagctcgat tctactcgag cgtgctttcg ttgctcgaga gattaccttg
     1141 ttaaatccgg tttggatcca ttccaagaat actggtaaac cgttttacgc cattcttcat
     1201 aggattgatg ttggtgttgt tattgattta gagccagcta gaactgaaga tcctgcgctt
     1261 tctattgctg gtgctgttca atcgcagaaa ctcgcggttc gtgcgatttc tcagttacag
     1321 gctcttcctg gtggagatat taagcttttg tgtgacactg tcgtggaaag tgtgagggac
     1381 ttgactggtt atgatcgtgt tatggtttat aagtttcatg aagatgagca tggagaagtt
     1441 gtagctgaga gtaaacgaga cgatttagag ccttatattg gactgcatta tcctgctact
     1501 gatattcctc aagcgtcaag gttcttgttt aagcagaacc gtgtccgaat gatagtagat
     1561 tgcaatgcca cacctgttct tgtggtccag gacgataggc taactcagtc tatgtgcttg
     1621 gttggttcta ctcttagggc tcctcatggt tgtcactctc agtatatggc taacatggga
     1681 tctattgcgt ctttagcaat ggcggttata atcaatggaa atgaagatga tgggagcaat
     1741 gtagctagtg gaagaagctc gatgaggctt tggggtttgg ttgtttgcca tcacacttct
     1801 tctcgctgca taccgtttcc gctaaggtat gcttgtgagt ttttgatgca ggctttcggt
     1861 ttacagttaa acatggaatt gcagttagct ttgcaaatgt cagagaaacg cgttttgaga
     1921 acgcagacac tgttatgtga tatgcttctg cgtgactcgc ctgctggaat tgttacacag
     1981 agtcccagta tcatggactt agtgaaatgt gacggtgcag catttcttta ccacgggaag
     2041 tattacccgt tgggtgttgc tcctagtgaa gttcagataa aagatgttgt ggagtggttg
     2101 cttgcgaatc atgcggattc aaccggatta agcactgata gtttaggcga tgcggggtat
     2161 cccggtgcag ctgcgttagg ggatgctgtg tgcggtatgg cagttgcata tatcacaaaa
     2221 agagactttc ttttttggtt tcgatctcac actgcgaaag aaatcaaatg gggaggcgct
     2281 aagcatcatc cggaggataa agatgatggg caacgaatgc atcctcgttc gtcctttcag
     2341 gcttttcttg aagttgttaa gagccggagt cagccatggg aaactgcgga aatggatgcg
     2401 attcactcgc tccagcttat tctgagagac tcttttaaag aatctggtgg ttctgccggt
     2461 ggctccggtt ctggctccag cggtggcagc tctggtgcgt ccggcacggg tactgcgggt
     2521 ggcactggca gcggttccgg tactggctct ggcaaccgga aatggtttcc cgcagaacct
     2581 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaactatc
     2641 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
     2701 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
     2761 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
     2821 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
     2881 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
     2941 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
     3001 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
     3061 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
     3121 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
     3181 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
     3241 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
     3301 atcatgcaag ctggtggctg gaccaatgta aatattgtca tgaactatat ccgtaacctg
     3361 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgatggaag cggagctact
     3421 aacttcagcc tgctgaagca ggctggagac gtggaggaga accctggacc tatgcccaag
     3481 aagaagagga aggtgatgtt cttaccaacc gattattgtt gcaggttaag cgatcaagag
     3541 tatatggagc ttgtgtttga gaatggccag attcttgcaa agggccaaag atccaacgtt
     3601 tctctgcata atcaacgtac caaatcgatc atggatttgt atgaggcaga gtataacgag
     3661 gatttcatga agagtatcat ccatggtggt ggtggtgcca tcacaaatct cggggacacg
     3721 caggttgttc cacaaagtca tgttgctgct gcccatgaaa caaacatgtt ggaaagcaat
     3781 aaacatgttg acggtggttc tgccggtggc tccggttctg gctccagcgg tggcagctct
     3841 ggtgcgtccg gcacgggtac tgcgggtggc actggcagcg gttccggtac tggctctggc
     3901 ctgactgtgc accaaaacct gcctgccctc cctgtggatg ccacctctga tgaagtcagg
     3961 aagaacctga tggacatgtt cagggacagg caggccttct ctgaacacac ctggaagatg
     4021 ctcctgtctg tgtgcagatc ctgggctgcc tggtgcaagc tgaactgaac ccagctttct
     4081 tgtacaaagt ggtagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     4141 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     4201 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     4261 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     4321 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     4381 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     4441 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
     4501 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     4561 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     4621 cggtaggcgt gtacggtggg aggtctatat aagcagagct ggtttagtga accgtcagat
     4681 cgccaccatg agcgagctga ttaaggagaa catgcacatg aagctgtaca tggagggcac
     4741 cgtggacaac catcacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac
     4801 ccagaccatg agaatcaagg tggtcgaggg cggccctctc cccttcgcct tcgacatcct
     4861 ggctactagc ttcctctacg gcagcaagac cttcatcaac cacacccagg gcatccccga
     4921 cttcttcaag cagtccttcc ctgagggctt cacatgggag agagtcacca catacgaaga
     4981 cgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc tcatctacaa
     5041 cgtcaagatc agaggggtga acttcacatc caacggccct gtgatgcaga agaaaacact
     5101 cggctgggag gccttcaccg agacgctgta ccccgctgac ggcggcctgg aaggcagaaa
     5161 cgacatggcc ctgaagctcg tgggcgggag ccatctgatc gcaaacgcca agaccacata
     5221 tagatccaag aaacccgcta agaacctcaa gatgcctggc gtctactatg tggactacag
     5281 actggaaaga atcaaggagg ccaacaacga gacctacgtc gagcagcacg aggtggcagt
     5341 ggccagatac tgcgacctcc ctagcaaact ggggcacaag cttaatggaa gcggagaggg
     5401 caggggaagt cttctaacat gcggggacgt ggaggaaaat cccggcccca tgaccgagta
     5461 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     5521 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     5581 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     5641 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     5701 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     5761 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     5821 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     5881 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     5941 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     6001 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac aactttatta
     6061 tacatagttg atcaattccg ataatcaacc tctggattac aaaatttgtg aaagattgac
     6121 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     6181 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     6241 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
     6301 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
     6361 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
     6421 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct
     6481 gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
     6541 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
     6601 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
     6661 cgcctccccg catcgggaat tcccgcggtt cgctttaaga ccaatgactt acaaggcagc
     6721 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     6781 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     6841 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     6901 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     6961 acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca
     7021 gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc
     7081 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     7141 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta
     7201 gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     7261 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     7321 gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc
     7381 cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg
     7441 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     7501 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     7561 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     7621 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     7681 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     7741 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     7801 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     7861 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     7921 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     7981 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     8041 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     8101 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     8161 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     8221 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     8281 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     8341 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     8401 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     8461 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     8521 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     8581 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     8641 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     8701 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     8761 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     8821 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     8881 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     8941 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     9001 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     9061 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     9121 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     9181 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     9241 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     9301 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     9361 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     9421 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     9481 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     9541 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     9601 agagagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     9661 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     9721 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     9781 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     9841 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     9901 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     9961 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
    10021 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
    10081 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
    10141 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa
    10201 ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta tgcaatactc
    10261 ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg agagaaaaag
    10321 caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa
    10381 cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag agatattgta
    10441 tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat ctgagcctgg
    10501 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
    10561 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
    10621 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
    10681 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
    10741 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
    10801 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
    10861 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
    10921 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
    10981 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
    11041 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
    11101 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
    11161 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
    11221 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
    11281 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
    11341 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
    11401 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
    11461 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
    11521 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
    11581 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
    11641 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
    11701 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
    11761 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
    11821 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
    11881 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
    11941 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
    12001 tccattcgat tagtgaacgg atctcgacgg tatcgctagc ttttaaaaga aaagggggga
    12061 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
    12121 aagaattaca aaaacaaatt acaaaaattc aaaattttac tagtatcaac tttgtataga
    12181 aaagttgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
    12241 gcccattgac gtcaatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
    12301 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc
    12361 ctattgacgt caatgacggt aaatggcccg cctggcattg tgcccagtac atgaccttat
    12421 gggactttcc tacttggcag tacatctacg tatta
//
