LOCUS       sequence-268072-        7320 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human Retinoic Acid Receptor-beta and Human Retinoid X 
            Receptor-alpha in pcDNA3.1(+) plasmid expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7320)
  AUTHORS   Zolfaghari R, Mattie FJ, Wei CH, Chisholm DR, Whiting A, Ross AC
  TITLE     CYP26A1 gene promoter is a useful tool for reporting RAR-mediated 
            retinoid activity.
  JOURNAL   Anal Biochem. 2019 Jul 15;577:98-109. doi: 10.1016/j.ab.2019.04.022.
            Epub 2019 Apr 27.
  PUBMED    31039331
REFERENCE   2  (bases 1 to 7320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem."; date: "2019-07-15"; volume: "577:98-109. doi"; pages: " 
            10.1016/j.ab.2019.04.022. Epub 2019 Apr 27"
COMMENT     Sequence Label: pcDNA3.1-hRAR-beta.hRXR-alpha
FEATURES             Location/Qualifiers
     source          1..7320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2384..2401)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2390..2614
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      2660..3088
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2747..2766)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2957..2978
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3097..3117)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3115..3412
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     primer_bind     3344..3363
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     regulatory      3439..3448
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    4993..5114
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5030..5049)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5084..5103
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5163..5179)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5163..5179)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5176..5198)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5187..5203
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5211..5217,5218..5235,5236..5241))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5256..5277
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5394..5411)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5565..6153)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5645..5664)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6324..7115,7116..7184))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6947..6966
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7185..7289)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gccaccatga ccaccagcgg ccacgcatgt ccggtcccag cagtgaacgg acacatgact
      961 cactatccag ccacacccta cccgttactc tttccacctg tcatcggagg actttccctg
     1021 cctcccctcc atggccttca tggacatccg cctccgagtg gatgcagcac cccgtcgccg
     1081 gcaacaattg aaacacagag caccagctct gaggaactcg tcccaagccc cccatctcca
     1141 cttcctcccc ctcgagtgta caaaccctgc ttcgtctgcc aggacaaatc atcagggtac
     1201 cactatgggg tcagcgcctg tgagggatgt aagggctttt tccgcagaag tattcagaag
     1261 aatatgattt acacttgtca ccgagataag aactgtgtta ttaataaagt caccaggaat
     1321 cgatgccaat actgtcgact ccagaagtgc tttgaagtgg gaatgtccaa agaatctgtc
     1381 aggaatgaca ggaacaagaa aaagaaggag acttcgaagc aagaatgcac agagagctat
     1441 gaaatgacag ctgagttgga cgatctcaca gagaagatcc gaaaagctca ccaggaaact
     1501 ttcccttcac tctgccagct gggtaaatac accacgaatt ccagtgctga ccatcgagtc
     1561 cgactggacc tgggcctctg ggacaaattc agtgaactgg ccaccaagtg cattattaag
     1621 atcgtggagt ttgctaaacg tctgcctggt ttcactggct tgaccatcgc agaccaaatt
     1681 accctgctga aggccgcctg cctggacatc ctgattctta gaatttgcac caggtatacc
     1741 ccagaacaag acaccatgac tttctcagac ggccttaccc taaatcgaac tcagatgcac
     1801 aatgctggat ttggtcctct gactgacctt gtgttcacct ttgccaacca gctcctgcct
     1861 ttggaaatgg atgacacaga aacaggcctt ctcagtgcca tctgcttaat ctgtggagac
     1921 cgccaggacc ttgaggaacc gacaaaagta gataagctac aagaaccatt gctggaagca
     1981 ctaaaaattt atatcagaaa aagacgaccc agcaagcctc acatgtttcc aaagatctta
     2041 atgaaaatca cagatctccg tagcatcagt gctaaaggtg cagagcgtgt aattaccttg
     2101 aaaatggaaa ttcctggatc aatgccacct ctcattcaag aaatgctgga gaattctgaa
     2161 ggacatgaac ccttgacccc aagttcaagt gggaacacag cagagcacag tcctagcatc
     2221 tcacccagct cagtggaaaa cagtggggtc agtcagtcac cactcgtgca ataagcttgg
     2281 taccgagctc ggatccacta gtccagtgtg gtggaattct gcagatatcc agcacagtgg
     2341 cggccgctcg agtctagagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct
     2401 agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc
     2461 actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt
     2521 cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat
     2581 agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg
     2641 ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     2701 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     2761 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     2821 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     2881 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     2941 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     3001 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     3061 ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg
     3121 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     3181 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     3241 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     3301 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     3361 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggatctg
     3421 cagaattcac tagtgattgc caccatggac accaaacatt tcctgccgct cgatttctcc
     3481 acccaggtga actcctccct cacctccccg acggggcgag gctccatggc tgccccctcg
     3541 ctgcacccgt ccctggggcc tggcatcggc tccccgggac agctgcattc tcccatcagc
     3601 accctgagct cccccatcaa cggcatgggc ccgcctttct cggtcatcag ctcccccatg
     3661 ggcccccact ccatgtcggt gcccaccaca cccaccctgg gcttcagcac tggcagcccc
     3721 cagctcagct cacctatgaa ccccgtcagc agcagcgagg acatcaagcc ccccctgggc
     3781 ctcaatggcg tcctcaaggt ccccgcccac ccctcaggaa acatggcttc cttcaccaag
     3841 cacatctgcg ccatctgcgg ggaccgctcc tcaggcaagc actatggagt gtacagctgc
     3901 gaggggtgca agggcttctt caagcggacg gtgcgcaagg acctgaccta cacctgccgc
     3961 gacaacaagg actgcctgat tgacaagcgg cagcggaacc ggtgccagta ctgccgctac
     4021 cagaagtgcc tggccatggg catgaagcgg gaagccgtgc aggaggagcg gcagcgtggc
     4081 aaggaccgga acgagaatga ggtggagtcg accagcagcg ccaacgagga catgccggtg
     4141 gagaggatcc tggaggctga gctggccgtg gagcccaaga ccgagaccta cgtggaggca
     4201 aacatggggc tgaaccccag ctcgccgaac gaccctgtca ccaacatttg ccaagcagcc
     4261 gacaaacagc ttttcaccct ggtggagtgg gccaagcgga tcccacactt ctcagagctg
     4321 cccctggacg accaggtcat cctgctgcgg gcaggctgga atgagctgct catcgcctcc
     4381 ttctcccacc gctccatcgc cgtgaaggac gggatcctcc tggccaccgg gctgcacgtc
     4441 caccggaaca gcgcccacag cgcaggggtg ggcgccatct ttgacagggt gctgacggag
     4501 cttgtgtcca agatgcggga catgcagatg gacaagacgg agctgggctg cctgcgcgcc
     4561 atcgtcctct ttaaccctga ctccaagggg ctctcgaacc cggccgaggt ggaggcgctg
     4621 agggagaagg tctatgcgtc cttggaggcc tactgcaagc acaagtaccc agagcagccg
     4681 ggaaggttcg ctaagctctt gctccgcctg ccggctctgc gctccatcgg gctcaaatgc
     4741 ctggaacatc tcttcttctt caagctcatc ggggacacac ccattgacac cttccttatg
     4801 gagatgctgg aggcgccgca ccaaatgact tagttcgaaa tgaccgacca agcgacgccc
     4861 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     4921 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     4981 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     5041 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     5101 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     5161 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     5221 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     5281 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     5341 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     5401 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     5461 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     5521 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     5581 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     5641 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     5701 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     5761 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     5821 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     5881 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     5941 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     6001 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     6061 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     6121 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     6181 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     6241 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     6301 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     6361 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     6421 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     6481 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     6541 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     6601 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     6661 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     6721 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     6781 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     6841 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     6901 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     6961 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     7021 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     7081 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     7141 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     7201 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     7261 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
