LOCUS       sequence-268075-        6956 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian, Zebrafish, or Xenopus expression of PhyB-Delta-CreC, one 
            half of the CreLite system..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6956)
  AUTHORS   Yen ST, Trimmer KA, Aboul-Fettouh N, Mullen RD, Culver JC, Dickinson
            ME, Behringer RR, Eisenhoffer GT
  TITLE     CreLite: An optogenetically controlled Cre/loxP system using red 
            light.
  JOURNAL   Dev Dyn. 2020 Nov;249(11):1394-1403. doi: 10.1002/dvdy.232. Epub 
            2020 Aug 31.
  PUBMED    32745301
REFERENCE   2  (bases 1 to 6956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/dvdy.232"; 
            journalName: "Dev Dyn"; date: "2020-11"; volume: "249"; issue: "11";
            pages: "1394-1403"
COMMENT     Sequence Label: pCS2+PhyB-Delta-CreC
FEATURES             Location/Qualifiers
     source          1..6956
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        845..863
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     845..862
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             934..954
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3818..3952
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3842..3861)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3896..3915
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4065..4085)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4065..4083)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4104..4120)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4104..4120)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4117..4139)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4128..4144
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4152..4158,4159..4176,4177..4182))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4197..4218
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4335..4352)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4506..5094)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4586..4605)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5265..6056,6057..6125))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5888..5907
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6126..6230)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6256..6711)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6393..6414)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6605..6624
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        join(6782..6956,1..810)
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 acctggcact gtgccaactg gggaggggtc tacttggcac ggtgccaagt ttgaggaggg
       61 gtcttggccc tgtgccaagt ccgccatatt gaattggcat ggtgccaata atggcggcca
      121 tattggctat atgccaggat caatatatag gcaatatcca atatggccct atgccaatat
      181 ggctattggc caggttcaat actatgtatt ggccctatgc catatagtat tccatatatg
      241 ggttttccta ttgacgtaga tagcccctcc caatgggcgg tcccatatac catatatggg
      301 gcttcctaat accgcccata gccactcccc cattgacgtc aatggtctct atatatggtc
      361 tttcctattg acgtcatatg ggcggtccta ttgacgtata tggcgcctcc cccattgacg
      421 tcaattacgg taaatggccc gcctggctca atgcccattg acgtcaatag gaccacccac
      481 cattgacgtc aatgggatgg ctcattgccc attcatatcc gttctcacgc cccctattga
      541 cgtcaatgac ggtaaatggc ccacttggca gtacatcaat atctattaat agtaacttgg
      601 caagtacatt actattggaa gtacgccagg gtacattggc agtactccca ttgacgtcaa
      661 tggcggtaaa tggcccgcga tggctgccaa gtacatcccc attgacgtca atggggaggg
      721 gcaatgacgc aaatgggcgt tccattgacg taaatgggcg gtaggcgtgc ctaatgggag
      781 gtctatataa gcaatgctcg tttagggaac cgccattctg cctggggacg tcggagcaag
      841 cttgatttag gtgacactat agaatacaag ctacttgttc tttttgcagg atcccatcga
      901 ttcgaattcc tcgaggatat cgccgccacc atgcccaaga agaagaggaa ggtggtttcc
      961 ggagtcgggg gtagtggcgg tggccgtggc ggtggccgtg gcggagaaga agaaccgtcg
     1021 tcaagtcaca ctcctaataa ccgaagagga ggagaacaag ctcaatcgtc gggaacgaaa
     1081 tctctcagac caagaagcaa cactgaatca atgagcaaag caattcaaca gtacaccgtc
     1141 gacgcaagac tccacgccgt tttcgaacaa tccggcgaat cagggaaatc attcgactac
     1201 tcacaatcac tcaaaacgac gacgtacggt tcctctgtac ctgagcaaca gatcacagct
     1261 tatctctctc gaatccagcg aggtggttac attcagcctt tcggatgtat gatcgccgtc
     1321 gatgaatcca gtttccggat catcggttac agtgaaaacg ccagagaaat gttagggatt
     1381 atgcctcaat ctgttcctac tcttgagaaa cctgagattc tagctatggg aactgatgtg
     1441 agatctttgt tcacttcttc gagctcgatt ctactcgagc gtgctttcgt tgctcgagag
     1501 attaccttgt taaatccggt ttggatccat tccaagaata ctggtaaacc gttttacgcc
     1561 attcttcata ggattgatgt tggtgttgtt attgatttag agccagctag aactgaagat
     1621 cctgcgcttt ctattgctgg tgctgttcaa tcgcagaaac tcgcggttcg tgcgatttct
     1681 cagttacagg ctcttcctgg tggagatatt aagcttttgt gtgacactgt cgtggaaagt
     1741 gtgagggact tgactggtta tgatcgtgtt atggtttata agtttcatga agatgagcat
     1801 ggagaagttg tagctgagag taaacgagac gatttagagc cttatattgg actgcattat
     1861 cctgctactg atattcctca agcgtcaagg ttcttgttta agcagaaccg tgtccgaatg
     1921 atagtagatt gcaatgccac acctgttctt gtggtccagg acgataggct aactcagtct
     1981 atgtgcttgg ttggttctac tcttagggct cctcatggtt gtcactctca gtatatggct
     2041 aacatgggat ctattgcgtc tttagcaatg gcggttataa tcaatggaaa tgaagatgat
     2101 gggagcaatg tagctagtgg aagaagctcg atgaggcttt ggggtttggt tgtttgccat
     2161 cacacttctt ctcgctgcat accgtttccg ctaaggtatg cttgtgagtt tttgatgcag
     2221 gctttcggtt tacagttaaa catggaattg cagttagctt tgcaaatgtc agagaaacgc
     2281 gttttgagaa cgcagacact gttatgtgat atgcttctgc gtgactcgcc tgctggaatt
     2341 gttacacaga gtcccagtat catggactta gtgaaatgtg acggtgcagc atttctttac
     2401 cacgggaagt attacccgtt gggtgttgct cctagtgaag ttcagataaa agatgttgtg
     2461 gagtggttgc ttgcgaatca tgcggattca accggattaa gcactgatag tttaggcgat
     2521 gcggggtatc ccggtgcagc tgcgttaggg gatgctgtgt gcggtatggc agttgcatat
     2581 atcacaaaaa gagactttct tttttggttt cgatctcaca ctgcgaaaga aatcaaatgg
     2641 ggaggcgcta agcatcatcc ggaggataaa gatgatgggc aacgaatgca tcctcgttcg
     2701 tcctttcagg cttttcttga agttgttaag agccggagtc agccatggga aactgcggaa
     2761 atggatgcga ttcactcgct ccagcttatt ctgagagact cttttaaaga atctggtggt
     2821 tctgccggtg gctccggttc tggctccagc ggtggcagct ctggtgcgtc cggcacgggt
     2881 actgcgggtg gcactggcag cggttccggt actggctctg gcaaccggaa atggtttccc
     2941 gcagaacctg aagatgttcg cgattatctt ctatatcttc aggcgcgcgg tctggcagta
     3001 aaaactatcc agcaacattt gggccagcta aacatgcttc atcgtcggtc cgggctgcca
     3061 cgaccaagtg acagcaatgc tgtttcactg gttatgcggc ggatccgaaa agaaaacgtt
     3121 gatgccggtg aacgtgcaaa acaggctcta gcgttcgaac gcactgattt cgaccaggtt
     3181 cgttcactca tggaaaatag cgatcgctgc caggatatac gtaatctggc atttctgggg
     3241 attgcttata acaccctgtt acgtatagcc gaaattgcca ggatcagggt taaagatatc
     3301 tcacgtactg acggtgggag aatgttaatc catattggca gaacgaaaac gctggttagc
     3361 accgcaggtg tagagaaggc acttagcctg ggggtaacta aactggtcga gcgatggatt
     3421 tccgtctctg gtgtagctga tgatccgaat aactacctgt tttgccgggt cagaaaaaat
     3481 ggtgttgccg cgccatctgc caccagccag ctatcaactc gcgccctgga agggattttt
     3541 gaagcaactc atcgattgat ttacggcgct aaggatgact ctggtcagag atacctggcc
     3601 tggtctggac acagtgcccg tgtcggagcc gcgcgagata tggcccgcgc tggagtttca
     3661 ataccggaga tcatgcaagc tggtggctgg accaatgtaa atattgtcat gaactatatc
     3721 cgtaacctgg atagtgaaac aggggcaatg gtgcgcctgc tggaagatgg cgattaggcg
     3781 gtcgagcctc tagaactata gtgagtcgta ttacgtagat ccagacatga taagatacat
     3841 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     3901 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     3961 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaattcgc
     4021 ggccgcggcg ccaatgcatt gggcccggta cccagctttt gttcccttta gtgagggtta
     4081 attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     4141 acaattccac acaacatacg agccgggagc ataaagtgta aagcctgggg tgcctaatga
     4201 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     4261 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     4321 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     4381 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     4441 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     4501 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     4561 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     4621 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     4681 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     4741 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     4801 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     4861 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     4921 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     4981 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     5041 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     5101 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     5161 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     5221 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     5281 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     5341 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     5401 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     5461 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     5521 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     5581 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     5641 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     5701 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     5761 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     5821 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     5881 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     5941 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     6001 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     6061 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     6121 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     6181 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     6241 cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
     6301 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
     6361 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
     6421 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
     6481 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
     6541 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
     6601 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
     6661 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc
     6721 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     6781 agtcgaccgc caattcaata tggcgtatat ggactcatgc caattcaata tggtggatct
     6841 ggacctgtgc caattcaata tggcgtatat ggactcgtgc caattcaata tggtggatct
     6901 ggaccccagc caattcaata tggcggactt ggcaccatgc caattcaata tggcgg
//
