LOCUS       sequence-268079-        4682 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian, Zebrafish, or Xenopus expression of PIF6CreN, one half of
            the CreLite system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4682)
  AUTHORS   Yen ST, Trimmer KA, Aboul-Fettouh N, Mullen RD, Culver JC, Dickinson
            ME, Behringer RR, Eisenhoffer GT
  TITLE     CreLite: An optogenetically controlled Cre/loxP system using red 
            light.
  JOURNAL   Dev Dyn. 2020 Nov;249(11):1394-1403. doi: 10.1002/dvdy.232. Epub 
            2020 Aug 31.
  PUBMED    32745301
REFERENCE   2  (bases 1 to 4682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/dvdy.232"; 
            journalName: "Dev Dyn"; date: "2020-11"; volume: "249"; issue: "11";
            pages: "1394-1403"
COMMENT     Sequence Label: pCS2+PIF6CreN
FEATURES             Location/Qualifiers
     source          1..4682
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        845..863
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     845..862
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             913..933
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1544..1678
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1568..1587)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1622..1641
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1791..1811)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1791..1809)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1830..1846)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1830..1846)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1843..1865)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1854..1870
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1878..1884,1885..1902,1903..1908))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1923..1944
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2061..2078)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2232..2820)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2312..2331)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2991..3782,3783..3851))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3614..3633
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3852..3956)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3982..4437)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4119..4140)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4331..4350
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        join(4508..4682,1..810)
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 acctggcact gtgccaactg gggaggggtc tacttggcac ggtgccaagt ttgaggaggg
       61 gtcttggccc tgtgccaagt ccgccatatt gaattggcat ggtgccaata atggcggcca
      121 tattggctat atgccaggat caatatatag gcaatatcca atatggccct atgccaatat
      181 ggctattggc caggttcaat actatgtatt ggccctatgc catatagtat tccatatatg
      241 ggttttccta ttgacgtaga tagcccctcc caatgggcgg tcccatatac catatatggg
      301 gcttcctaat accgcccata gccactcccc cattgacgtc aatggtctct atatatggtc
      361 tttcctattg acgtcatatg ggcggtccta ttgacgtata tggcgcctcc cccattgacg
      421 tcaattacgg taaatggccc gcctggctca atgcccattg acgtcaatag gaccacccac
      481 cattgacgtc aatgggatgg ctcattgccc attcatatcc gttctcacgc cccctattga
      541 cgtcaatgac ggtaaatggc ccacttggca gtacatcaat atctattaat agtaacttgg
      601 caagtacatt actattggaa gtacgccagg gtacattggc agtactccca ttgacgtcaa
      661 tggcggtaaa tggcccgcga tggctgccaa gtacatcccc attgacgtca atggggaggg
      721 gcaatgacgc aaatgggcgt tccattgacg taaatgggcg gtaggcgtgc ctaatgggag
      781 gtctatataa gcaatgctcg tttagggaac cgccattctg cctggggacg tcggagcaag
      841 cttgatttag gtgacactat agaatacaag ctacttgttc tttttgcagg atcccatcga
      901 ttcgaattca tgcccaagaa gaagaggaag gtgatgttct taccaaccga ttattgttgc
      961 aggttaagcg atcaagagta tatggagctt gtgtttgaga atggccagat tcttgcaaag
     1021 ggccaaagat ccaacgtttc tctgcataat caacgtacca aatcgatcat ggatttgtat
     1081 gaggcagagt ataacgagga tttcatgaag agtatcatcc atggtggtgg tggtgccatc
     1141 acaaatctcg gggacacgca ggttgttcca caaagtcatg ttgctgctgc ccatgaaaca
     1201 aacatgttgg aaagcaataa acatgttgac ggtggttctg ccggtggctc cggttctggc
     1261 tccagcggtg gcagctctgg tgcgtccggc acgggtactg cgggtggcac tggcagcggt
     1321 tccggtactg gctctggcct gactgtgcac caaaacctgc ctgccctccc tgtggatgcc
     1381 acctctgatg aagtcaggaa gaacctgatg gacatgttca gggacaggca ggccttctct
     1441 gaacacacct ggaagatgct cctgtctgtg tgcagatcct gggctgcctg gtgcaagctg
     1501 aactgactcg agcctctaga actatagtga gtcgtattac gtagatccag acatgataag
     1561 atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat gctttatttg
     1621 tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa
     1681 caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg aggtttttta
     1741 attcgcggcc gcggcgccaa tgcattgggc ccggtaccca gcttttgttc cctttagtga
     1801 gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     1861 ccgctcacaa ttccacacaa catacgagcc gggagcataa agtgtaaagc ctggggtgcc
     1921 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     1981 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     2041 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     2101 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     2161 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     2221 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     2281 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     2341 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     2401 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     2461 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     2521 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     2581 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     2641 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     2701 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     2761 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     2821 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     2881 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     2941 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     3001 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     3061 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     3121 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     3181 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     3241 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     3301 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     3361 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     3421 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     3481 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     3541 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     3601 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     3661 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     3721 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     3781 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     3841 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     3901 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     3961 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     4021 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     4081 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     4141 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     4201 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     4261 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     4321 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     4381 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
     4441 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     4501 tacgccagtc gaccgccaat tcaatatggc gtatatggac tcatgccaat tcaatatggt
     4561 ggatctggac ctgtgccaat tcaatatggc gtatatggac tcgtgccaat tcaatatggt
     4621 ggatctggac cccagccaat tcaatatggc ggacttggca ccatgccaat tcaatatggc
     4681 gg
//
