LOCUS       sequence-268181-         660 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of VP16-ZF21 under the CMV promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Donahue PS, Draut JW, Muldoon JJ, Edelstein HI, Bagheri N, Leonard 
            JN
  TITLE     The COMET toolkit for composing customizable genetic programs in 
            mammalian cells.
  JOURNAL   Nat Commun. 2020 Feb 7;11(1):779. doi: 10.1038/s41467-019-14147-5.
  PUBMED    32034124
REFERENCE   2  (bases 1 to 660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-02-7"; volume: "11(1):779. doi"; pages: " 
            10.1038/s41467-019-14147-5"
COMMENT     Sequence Label: pPD528 VP16-ZF21
FEATURES             Location/Qualifiers
     source          1..660
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             76..96
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atggattata aggatcacga tggcgactac aaggaccacg atattgacta caaagacgat
       61 gacgataaaa tggctcctaa gaaaaagcgc aaagtcggta tccatggcgt tcccggatcc
      121 gtcgacggtg gactgcaggc cccaccaacc gatgtttctt taggtgatga gttacatttg
      181 gacggtgagg acgtagctat ggcacatgca gatgccttag acgattttga cttggatatg
      241 ttaggagatg gagattcacc aggacctggt ttcacaccac acgattcagc tccatacggt
      301 gctttggaca tggccgattt cgaatttgaa cagatgttta ctgatgcctt gggtatagac
      361 gaatacggtg gtggaggtac cggtggagct agacccgggg agcgcccctt ccagtgtcgc
      421 atttgcatgc ggaacttttc gagaaatttc attttgcaaa gacatacccg tactcatacc
      481 ggtgaaaaac cgtttcagtg tcggatctgt atgcgaaatt tctcccaagg tggtaatttg
      541 gttagacatc tacgtacgca caccggcgag aagccattcc aatgccgaat atgcatgcgc
      601 aacttcagtc aacaaactgg tttgaatgtt cacctaaaaa cccacctgag aggatcttaa
//
