LOCUS       sequence-268209-         642 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of VP16-ZF158 under the CMV promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Donahue PS, Draut JW, Muldoon JJ, Edelstein HI, Bagheri N, Leonard 
            JN
  TITLE     The COMET toolkit for composing customizable genetic programs in 
            mammalian cells.
  JOURNAL   Nat Commun. 2020 Feb 7;11(1):779. doi: 10.1038/s41467-019-14147-5.
  PUBMED    32034124
REFERENCE   2  (bases 1 to 642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-02-7"; volume: "11(1):779. doi"; pages: " 
            10.1038/s41467-019-14147-5"
COMMENT     Sequence Label: pPD250 VP16-ZF158
FEATURES             Location/Qualifiers
     source          1..642
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             76..96
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atggattata aggatcacga tggcgactac aaggaccacg atattgacta caaagacgat
       61 gacgataaaa tggctcctaa gaaaaagcgc aaagtcggta tccatggcgt tcccgtcgac
      121 ggtggactgc aggccccacc aaccgatgtt tctttaggtg atgagttaca tttggacggt
      181 gaggacgtag ctatggcaca tgcagatgcc ttagacgatt ttgacttgga tatgttagga
      241 gatggagatt caccaggacc tggtttcaca ccacacgatt cagctccata cggtgctttg
      301 gacatggccg atttcgaatt tgaacagatg tttactgatg ccttgggtat agacgaatac
      361 ggtggtggag ctagacccgg ggagcgcccc ttccagtgtc gcatttgcat gcggaacttt
      421 tcggataaaa ctaaattgag agttcatacc cgtactcata ccggtgaaaa accgtttcag
      481 tgtcggatct gtatgcgaaa tttctccgtt agacataatt tgactagaca tctacgtacg
      541 cacaccggcg agaagccatt ccaatgccga atatgcatgc gcaacttcag tcaatctact
      601 tctttgcaaa gacacctaaa aacccacctg agaggatctt aa
//
