LOCUS       sequence-26822-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Shen WF, Montgomery JC, Rozenfeld S, Moskow JJ, Lawrence HJ, 
            Buchberg AM, Largman C
  TITLE     AbdB-like Hox proteins stabilize DNA binding by the Meis1 
            homeodomain proteins
  JOURNAL   Mol Cell Biol. 1997 Nov;17(11):6448-58
  PUBMED    9343407
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "1997-11"; volume: "17(11)"; pages: "6448-5"
COMMENT     Sequence Label: MEIS1A-pET
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             27..49
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             68..85
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             95..112
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             116..148
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 gnnnannnnn nnccctctag aataattttg tttaacttta agaaggagat ataccatggg
       61 cagcagccat catcatcatc atcacagcag cggcctggtg ccgcgcggca gccatatggc
      121 tagcatgact ggtggacagc aaatgggtcg ggatccgaat tcgatggcgc aaaggtacga
      181 cgacctaccc cattatgggg gtatggatgg agtaggcatc ccctccacga tgtatgggga
      241 cccgcatgca gccaggtcca tgcaaccggt ccaccacctg aaccacgggc ctcctctgca
      301 ctcgcatcag tacccgcaca cagctcacac caacgccatg gcccccagca tgggttcctc
      361 ggtcaatgac gctttaaaga gagataaaga tgccatttat ggacaccccc tcttccctct
      421 cttagcactg atttttgaga aatgtgaatt agctacttgt accccccgcg agccgggggt
      481 ggcgggcggg gacgtctgct cgtcagagtc attcaatgaa gatatagcgg tgttcgccaa
      541 acagattcgc gcagaaaaac ctctattctc ttctaatcca gaactggata acttgatgat
      601 tcaagccata caagtgttaa ggtttcatct gttggaatta gagaaggtac acgaattatg
      661 tgacaatttc tgccaccggt atattagctg tttgaaaggg aaaatgccta tcgatttggt
      721 gatagatgat agagaaggag gatcaaaatc nnnnngtgaa nntgtaaaca agaatcagca
      781 aatcctaact gaccagcccn tcttggaaat nnaanaccaa tgatngaccn cggcaatncc
//
