LOCUS       sequence-268229-         417 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of ZF54i under the CMV promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 417)
  TITLE     Leonard Lab COMET plasmids
REFERENCE   2  (bases 1 to 417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD691 ZF54i
FEATURES             Location/Qualifiers
     source          1..417
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             76..96
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atggattata aggatcacga tggcgactac aaggaccacg atattgacta caaagacgat
       61 gacgataaaa tggctcctaa gaaaaagcgc aaagtcggta tccatggcgt tcccggatcc
      121 tctggctctt caggtaccgg tggagctaga cccggggagc gccccttcca gtgtcgcatt
      181 tgcatgcgga acttttcgaa taaaactgat ttgggtagac atacccgtac tcataccggt
      241 gaaaaaccgt ttcagtgtcg gatctgtatg cgaaatttct ccagaagaga tatgttgaga
      301 agacatctac gtacgcacac cggcgagaag ccattccaat gccgaatatg catgcgcaac
      361 ttcagtagaa tggatcattt ggctggtcac ctaaaaaccc acctgagagg atcttaa
//
