LOCUS       sequence-26823-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  TITLE     Largman Plasmids
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MEIS1A-pVP16
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      50..59
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             62..82
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             83..316
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
ORIGIN
        1 cattcagagt agtgaagagg cttttttgga ggagatctaa gctagcgccg ccaccatggg
       61 ccctaaaaag aagcgtaaag tcgccccccc gaccgatgtc agcctggggg acgagctcca
      121 cttagacggc gaggacgtgg cgatggcgca tgccgacgcg ctagacgatt tcgatctgga
      181 catgttgggg gacggggatt ccccgggtcc gggatttacc ccccacgact ccgcccccta
      241 cggcgctctg gatatggccg acttcgagtt tgagcagatg tttaccgatg cccttggaat
      301 tgacgagtac ggtggggaat tatcgaattc gatggcgcaa aggtacgacg acctacccca
      361 ttatgggggt atggatggag taggcatccc ctccacgatg tatggggacc cgcatgcagc
      421 caggtccatg caaccggtcc accacctgaa ccacgggcct cctctgcact cgcatcagta
      481 cccgcacaca gctcacacca acgccatggc ccccagcatg ggttcctcgg tcaatgacgc
      541 tttaaagaga gataaagatg ccatttatgg acaccccctc ttccctctct tagcactgat
      601 ttttgagaaa tgtgaattag ctacttgtac cccccgcgag ccgggggtgg cgggcgggga
      661 cgtctgctcg tcagagtcat tcaatgaaga tatagcggtg ttcgccaaac agattcgcgc
      721 agaaaaacct ctattctctt ctaatccaga actggataac ttgatgattc aagccataca
      781 agnngtttaa aggtttcatc ctgttggaat ttag
//
