LOCUS       sequence-268257-        1125 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of DsRed-Express2-ZF21 under the CMV 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1125)
  TITLE     Leonard Lab COMET plasmids
REFERENCE   2  (bases 1 to 1125)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD708 DsRed-Express2-ZF21
FEATURES             Location/Qualifiers
     source          1..1125
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             76..96
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             139..813
                     /codon_start=1
                     /product="noncytotoxic tetrameric variant of DsRed 
                     fluorescent protein (Strack et al., 2008)"
                     /label=DsRed-Express2
                     /note="mammalian codon-optimized"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     primer_bind     complement(319..339)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     731..754
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 atggattata aggatcacga tggcgactac aaggaccacg atattgacta caaagacgat
       61 gacgataaaa tggctcctaa gaaaaagcgc aaagtcggta tccatggcgt tcccggatca
      121 ggctccggaa gtggctccat ggatagcact gagaacgtca tcaagccctt catgcgcttc
      181 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
      241 ggcaagccct acgagggcac ccagaccgcc aagctccagg tgaccaaggg cggccccctg
      301 cccttcgcct gggacatcct gtccccccag ttccagtacg gctccaaggt gtacgtgaag
      361 caccccgccg acatccccga ctacaagaag ctgtccttcc ccgagggctt caagtgggag
      421 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctccag
      481 gacggcacct tcatctacca cgtgaagttc atcggcgtga acttcccctc cgacggcccc
      541 gtaatgcaga agaaaactct gggctgggag ccctccaccg agcgcctgta cccccgcgac
      601 ggcgtgctga agggcgagat ccacaaggcg ctgaagctga agggcggcgg ccactacctg
      661 gtggagttca agtcaatcta catggccaag aagcccgtga agctgcccgg ctactactac
      721 gtggactcca agctggacat cacctcccac aacgaggact acaccgtggt ggagcagtac
      781 gagcgcgccg aggcccgcca ccacctgttc cagtctggtt ctggttctgg ttcaggatcc
      841 ggtaccggtg gagctagacc cggggagcgc cccttccagt gtcgcatttg catgcggaac
      901 ttttcgagaa atttcatttt gcaaagacat acccgtactc ataccggtga aaaaccgttt
      961 cagtgtcgga tctgtatgcg aaatttctcc caaggtggta atttggttag acatctacgt
     1021 acgcacaccg gcgagaagcc attccaatgc cgaatatgca tgcgcaactt cagtcaacaa
     1081 actggtttga atgttcacct aaaaacccac ctgagaggat cttaa
//
