LOCUS       sequence-26826-e         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  TITLE     Largman Plasmids
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MEIS1B-pVP16
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      43..52
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             55..75
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             76..309
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
ORIGIN
        1 agtagtgaag aggctttttt ggaggagatc taagctagcg ccgccaccat gggccctaaa
       61 aagaagcgta aagtcgcccc cccgaccgat gtcagcctgg gggacgagct ccacttagac
      121 ggcgaggacg tggcgatggc gcatgccgac gcgctagacg atttcgatct ggacatgttg
      181 ggggacgggg attccccggg tccgggattt accccccacg actccgcccc ctacggcgct
      241 ctggatatgg ccgacttcga gtttgagcag atgtttaccg atgcccttgg aattgacgag
      301 tacggtgggg aattatcgaa ttcgatggcg caaaggtacg acgacctacc ccattatggg
      361 ggtatggatg gagtaggcat cccctccacg atgtatgggg acccgcatgc agccaggtcc
      421 atgcaaccgg tccaccacct gaaccacggg cctcctctgc actcgcatca gtacccgcac
      481 acagctcaca ccaacgccat ggcccccagc atgggttcct cggtcaatga cgctttaaag
      541 agagataaag atgccattta tggacacccc ctcttccctc tcttagcact gatttttgag
      601 aaatgtgaat tagctacttg taccccccgc gagccggggg tggcgggcgg ggacgtctgc
      661 tcgtcagagt cattcaatga agatatagcg gtgttcgccc aaacaggatt cncgcanaaa
      721 aacctcnntt ctcttctaat ccagaactgg attaacttga tgattcaagc catacaagtg
      781 tttaaggttt ca
//
