LOCUS       sequence-268325-         417 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of ZF43(RAAA)i under the CMV promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Donahue PS, Draut JW, Muldoon JJ, Edelstein HI, Bagheri N, Leonard 
            JN
  TITLE     The COMET toolkit for composing customizable genetic programs in 
            mammalian cells.
  JOURNAL   Nat Commun. 2020 Feb 7;11(1):779. doi: 10.1038/s41467-019-14147-5.
  PUBMED    32034124
REFERENCE   2  (bases 1 to 417)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-02-7"; volume: "11(1):779. doi"; pages: " 
            10.1038/s41467-019-14147-5"
COMMENT     Sequence Label: pPD169 ZF43(RAAA)i
FEATURES             Location/Qualifiers
     source          1..417
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             76..96
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 atggattata aggatcacga tggcgactac aaggaccacg atattgacta caaagacgat
       61 gacgataaaa tggctcctaa gaaaaagcgc aaagtcggta tccatggcgt tcccggatcc
      121 tctggctctt caggtaccgg tggagctaga cccggggagc gccccttcca gtgtcgcatt
      181 tgcatggcga acttttcgcg ccaggacagg cttgacaggc atacccgtac tcataccggt
      241 gaaaaaccgt ttcagtgtcg gatctgtatg gcaaatttct cccagaagga gcacttggcg
      301 gggcatctac gtacgcacac cggcgagaag ccattccaat gccgaatatg catggccaac
      361 ttcagtcgcc gcgacaacct gaaccggcac ctaaaaaccc acctgagagg atcttaa
//
